LOCUS       Exported               10972 bp ds-DNA     circular SYN 24-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    A3A-Gam
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 30, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..1999
                     /label=ubi promoter
     misc_feature    2018..2539
                     /label=Gam
     misc_feature    2540..2587
                     /label=XTEN
     misc_feature    2594..3187
                     /label=A3A
     misc_feature    3188..3283
                     /label=32aa
     CDS             3290..7390
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7391..7438
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    7466..7720
                     /label=UGI
     misc_feature    7742..8002
                     /label=UGI
     CDS             8003..8023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8053..8776
                     /label=CaMV term
     polyA_signal    8097..8273
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8816..8832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8840..8856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8864..8894)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8909..8930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9218..9806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9977..10837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10838..10942)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg gcgaagccgg ccaagaggat
     2041 caaatccgct gctgctgcct acgtgccgca aaatagggat gccgtgatca ccgacatcaa
     2101 gaggatcggc gatctgcaga gggaggcgtc tcgtctcgaa actgagatga acgacgcgat
     2161 cgcggagatc accgagaagt tcgccgctcg tatcgccccg atcaagaccg acatcgaaac
     2221 tctctccaag ggcgtgcaag gttggtgcga ggccaatagg gacgagctca ccaatggcgg
     2281 caaggtgaag accgccaacc tcgtgaccgg cgatgtgtct tggagggtga ggccaccatc
     2341 cgtgagcatt cgtggtatgg acgccgtgat ggaaactctc gagcgcctcg gcctccaaag
     2401 gttcatccgc accaagcaag aaatcaacaa ggaggcgatc ctcctcgagc caaaagccgt
     2461 ggccggcgtg gccggcatca cagtcaagtc cggcatcgag gacttctcca tcatcccgtt
     2521 cgagcaagaa gccggcatct ccggcagcga gacgccaggc acctccgaga gcgctacgcc
     2581 tgaatccagg cctgaggcca gcccggctag cggcccaagg catctcatgg acccgcacat
     2641 cttcaccagc aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt
     2701 ggagaggctc gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa
     2761 ccaagccaag aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga
     2821 cttggtgcca tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc
     2881 ctggtcccca tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa
     2941 cacccacgtc cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa
     3001 ggaggccctc cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga
     3061 gttcaagcac tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga
     3121 cggtctggat gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca
     3181 ggggaacagc ggaggatctt ccggaggatc tagcggctcc gagacaccag gaacatccga
     3241 aagcgctaca ccagaatcta gcggaggctc ttccggagga tctcttaagg acaagaagta
     3301 ctcgatcggc ctcgccatcg ggacgaactc agttggctgg gccgtgatca ccgacgagta
     3361 caaggtgccc tctaagaagt tcaaggtcct ggggaacacc gaccgccatt ccatcaagaa
     3421 gaacctcatc ggcgctctcc tgttcgacag cggggagacc gctgaggcta cgaggctcaa
     3481 gagaaccgct aggcgccggt acacgagaag gaagaacagg atctgctacc tccaagagat
     3541 tttctccaac gagatggcca aggttgacga ttcattcttc caccgcctgg aggagtcttt
     3601 cctcgtggag gaggataaga agcacgagcg gcatcccatc ttcggcaaca tcgtggacga
     3661 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
     3721 caccgataag gcggacctca gactgatcta cctcgctctg gcccacatga tcaagttccg
     3781 cggccatttc ctgatcgagg gggatctcaa cccagacaac agcgatgttg acaagctgtt
     3841 catccaactc gtgcagacct acaaccaact cttcgaggag aacccgatca acgcctctgg
     3901 cgtggacgcg aaggctatcc tgtccgcgag gctctcgaag tccaggaggc tggagaacct
     3961 gatcgctcag ctcccaggcg agaagaagaa cggcctgttc gggaacctca tcgctctcag
     4021 cctggggctc accccgaact tcaagtcgaa cttcgatctc gctgaggacg ccaagctgca
     4081 actctccaag gacacctacg acgatgacct cgataacctc ctggcccaga tcggcgatca
     4141 atacgcggac ctgttcctcg ctgccaagaa cctgtcggac gccatcctcc tgtcagatat
     4201 cctccgcgtg aacaccgaga tcacgaaggc tccactctct gcctccatga tcaagcgcta
     4261 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtccgccaac agctcccgga
     4321 gaagtacaag gagattttct tcgatcagtc gaagaacggc tacgctgggt acatcgacgg
     4381 cggggcctca caagaggagt tctacaagtt catcaagcca atcctggaga agatggacgg
     4441 cacggaggag ctcctggtga agctcaacag ggaggacctc ctgcggaagc agagaacctt
     4501 cgataacggc agcatccccc accaaatcca tctcggggag ctgcacgcca tcctgagaag
     4561 gcaagaggac ttctaccctt tcctcaagga taaccgggag aagatcgaga agatcctgac
     4621 cttcagaatc ccatactacg tcggccctct cgcgcggggg aactcaagat tcgcttggat
     4681 gacccgcaag tctgaggaga ccatcacgcc gtggaacttc gaggaggtgg tggacaaggg
     4741 cgctagcgct cagtcgttca tcgagaggat gaccaacttc gacaagaacc tgcccaacga
     4801 gaaggtgctc cctaagcact cgctcctgta cgagtacttc accgtctaca acgagctcac
     4861 gaaggtgaag tacgtcaccg agggcatgcg caagccagcg ttcctgtccg gggagcagaa
     4921 gaaggctatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcaactcaa
     4981 ggaggactac ttcaagaaga tcgagtgctt cgattcggtc gagatcagcg gcgttgagga
     5041 ccgcttcaac gccagcctcg ggacctacca cgatctcctg aagatcatca aggataagga
     5101 cttcctggac aacgaggaga acgaggatat cctggaggac atcgtgctga ccctcacgct
     5161 gttcgaggac agggagatga tcgaggagcg cctgaagacg tacgcccatc tcttcgatga
     5221 caaggtcatg aagcaactca agcgccggag atacaccggc tgggggaggc tgtcccgcaa
     5281 gctcatcaac ggcatccggg acaagcagtc cgggaagacc atcctcgact tcctcaagag
     5341 cgatggcttc gccaacagga acttcatgca actgatccac gatgacagcc tcaccttcaa
     5401 ggaggatatc caaaaggctc aagtgagcgg ccagggggac tcgctgcacg agcatatcgc
     5461 gaacctcgct ggctcccccg cgatcaagaa gggcatcctc cagaccgtga aggttgtgga
     5521 cgagctcgtg aaggtcatgg gccggcacaa gcctgagaac atcgtcatcg agatggccag
     5581 agagaaccaa accacgcaga aggggcaaaa gaactctagg gagcgcatga agcgcatcga
     5641 ggagggcatc aaggagctgg ggtcccaaat cctcaaggag cacccagtgg agaacaccca
     5701 actgcagaac gagaagctct acctgtacta cctccagaac ggcagggata tgtacgtgga
     5761 ccaagagctg gatatcaacc gcctcagcga ttacgacgtc gatcatatcg ttccccagtc
     5821 tttcctgaag gatgactcca tcgacaacaa ggtcctcacc aggtcggaca agaaccgcgg
     5881 caagtcagat aacgttccat ctgaggaggt cgttaagaag atgaagaact actggaggca
     5941 gctcctgaac gccaagctga tcacgcaaag gaagttcgac aacctcacca aggctgagag
     6001 aggcgggctc tcagagctgg acaaggccgg cttcatcaag cggcagctgg tcgagaccag
     6061 acaaatcacg aagcacgttg cgcaaatcct cgactctcgg atgaacacga agtacgatga
     6121 gaacgacaag ctgatcaggg aggttaaggt gatcaccctg aagtctaagc tcgtctccga
     6181 cttcaggaag gatttccagt tctacaaggt tcgcgagatc aacaactacc accatgccca
     6241 tgacgcttac ctcaacgctg tggtcggcac cgctctgatc aagaagtacc caaagctgga
     6301 gtccgagttc gtgtacgggg actacaaggt ttacgatgtg cgcaagatga tcgccaagtc
     6361 ggagcaagag atcggcaagg ctaccgccaa gtacttcttc tactcaaaca tcatgaactt
     6421 cttcaagacc gagatcacgc tggccaacgg cgagatccgg aagagaccgc tcatcgagac
     6481 caacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtccgcaa
     6541 ggttctctcc atgccccagg tgaacatcgt caagaagacc gaggtccaaa cgggcgggtt
     6601 ctcaaaggag tctatcctgc ctaagcggaa cagcgacaag ctcatcgcca gaaagaagga
     6661 ctgggaccca aagaagtacg gcgggttcga cagccctacc gtggcctact cggtcctggt
     6721 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctcctggg
     6781 gatcaccatc atggagaggt ccagcttcga gaagaaccca atcgacttcc tggaggccaa
     6841 gggctacaag gaggtgaaga aggacctgat catcaagctc ccgaagtact ctctcttcga
     6901 gctggagaac ggcaggaaga gaatgctggc ttccgctggc gagctccaga aggggaacga
     6961 gctcgcgctg ccaagcaagt acgtgaactt cctctacctg gcttcccact acgagaagct
     7021 caagggcagc ccggaggaca acgagcaaaa gcagctgttc gtcgagcagc acaagcatta
     7081 cctcgacgag atcatcgagc aaatctccga gttcagcaag cgcgtgatcc tcgccgacgc
     7141 gaacctggat aaggtcctct ccgcctacaa caagcaccgg gacaagccca tcagagagca
     7201 agcggagaac atcatccatc tcttcaccct gacgaacctc ggcgctcctg ctgctttcaa
     7261 gtacttcgac accacgatcg atcggaagag atacacctcc acgaaggagg tcctggacgc
     7321 gaccctcatc caccagtcga tcaccggcct gtacgagacg aggatcgacc tctcacaact
     7381 cggcggggat aagagacccg cagcaaccaa gaaggcaggg caagcaaaga agaagaagac
     7441 gcgttcaggc ggctccggcg gctccaccaa cctgtccgac atcatcgaga aggagacggg
     7501 caagcaactc gtgatccagg agagcatcct catgctgcca gaggaggtgg aggaggtcat
     7561 cggcaacaag ccagagtccg acatcctggt gcacaccgcc tacgacgagt ccaccgacga
     7621 gaacgtcatg ctcctgacca gcgacgcccc agagtacaag ccatgggccc tcgtcatcca
     7681 ggacagcaac ggggagaaca agatcaagat gctgtcgggg acgcgtgact ccggcggcag
     7741 caccaacctg tccgacatca tcgagaagga gacgggcaag caactcgtga tccaggagag
     7801 catcctcatg ctgccagagg aggtggagga ggtcatcggc aacaagccag agtccgacat
     7861 cctggtgcac accgcctacg acgagtccac cgacgagaac gtcatgctcc tgaccagcga
     7921 cgccccagag tacaagccat gggccctcgt catccaggac agcaacgggg agaacaagat
     7981 caagatgctg tcggggggga gcccaaagaa gaagcggaag gtgtagtgta caagtaaccc
     8041 gggaattcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc
     8101 tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc
     8161 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     8221 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atctcctaaa
     8281 gtccctatag atctttgtcg tgaatataaa ccagacacga gacgactaaa cctggagccc
     8341 agacgccgtt cgaagctaga agtaccgctt aggcaggagg ccgttaggga aaagatgcta
     8401 aggcagggtt ggttacgttg actcccccgt aggtttggtt taaatatgat gaagtggacg
     8461 gaaggaagga ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg taagaagatg
     8521 gaaatttgat agaggtacgc tactatactt atactatacg ctaagggaat gcttgtattt
     8581 ataccctata ccccctaata accccttatc aatttaagaa ataatccgca taagcccccg
     8641 cttaaaaatt ggtatcagag ccatgaatag gtctatgacc aaaactcaag aggataaaac
     8701 ctcaccaaaa tacgaaagag ttcttaactc taaagataaa agatctttca agatcaaaac
     8761 tagttccctc acaccggagc atgcgatatc ctcgagagat ctaggcgtaa tcatggtcat
     8821 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8881 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8941 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9001 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     9421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gt
//
