LOCUS       Exported               18697 bp ds-DNA     circular SYN 24-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pH-A3A-PBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 30, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..18697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..26
                     /label=RB
     promoter        274..710
                     /label=OsU3p
     CDS             990..1781
                     /codon_start=1
                     /label=SpR
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    1933..2008
                     /label=gRNA Sc
     terminator      2019..2299
                     /label=OsU3t plus
     misc_feature    2313..4303
                     /label=Ubi promoter
     misc_feature    4311..4955
                     /label=A3A
     CDS             4962..9062
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9063..9110
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    9111..9392
                     /label=UGI
     misc_feature    9393..9413
                     /label=sv40 NLS
     misc_feature    9423..10057
                     /label=E9ter
     CDS             11122..12147
                     /codon_start=1
                     /label=Hyg
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     misc_feature    12438..12463
                     /label=LB
     CDS             12888..13682
                     /codon_start=1
                     /label=KmR
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13973..14253
                     /direction=RIGHT
                     /label=pBR322 origin
     misc_feature    complement(15027..15221)
                     /label=pVS1 ori
     misc_feature    15227..15232
                     /label=Muatated BsaI
     CDS             complement(15287..16360)
                     /codon_start=1
                     /label=RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16789..17418)
                     /codon_start=1
                     /label=StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 acggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
     3181 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
     3241 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
     3301 gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     3361 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     3421 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     3481 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     3541 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     3601 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     3661 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     3721 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     3781 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     3841 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     3901 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     3961 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     4021 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     4081 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     4141 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     4201 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     4261 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagccctagg atggaggcca
     4321 gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc aacttcaaca
     4381 acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc gacaacggaa
     4441 cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag aacctcctct
     4501 gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca tccctccaac
     4561 tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca tgcttctcct
     4621 ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc cgcctccgca
     4681 ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc cagatgctgc
     4741 gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac tgctgggaca
     4801 ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat gaacacagcc
     4861 aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc gggagcgaga
     4921 cgccaggcac ctccgagtcg gccaccccag aatctaggcc tgacaagaag tactcgatcg
     4981 gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc
     5041 cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca
     5101 ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg
     5161 cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca
     5221 acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg
     5281 aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct
     5341 accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata
     5401 aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt
     5461 tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc
     5521 tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg
     5581 cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc
     5641 agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc
     5701 tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca
     5761 aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg
     5821 acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg
     5881 tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc
     5941 accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca
     6001 aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct
     6061 ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg
     6121 agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg
     6181 gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg
     6241 acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga
     6301 tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca
     6361 agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg
     6421 ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc
     6481 tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga
     6541 agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca
     6601 tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact
     6661 acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca
     6721 acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg
     6781 acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg
     6841 accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca
     6901 tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca
     6961 acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct
     7021 tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata
     7081 tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg
     7141 ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg
     7201 tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc
     7261 agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca
     7321 ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga
     7381 atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc
     7441 tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga
     7501 aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg
     7561 ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga
     7621 atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc
     7681 tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca
     7741 ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca
     7801 agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga
     7861 aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt
     7921 acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt
     7981 tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg
     8041 agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga
     8101 cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg
     8161 agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct
     8221 ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg
     8281 agtcaattct gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc
     8341 cgaagaagta cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga
     8401 aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga
     8461 ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca
     8521 aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga
     8581 acggcaggaa gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc
     8641 tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca
     8701 gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg
     8761 agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg
     8821 ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga
     8881 atatcattca tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg
     8941 acacaactat cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca
     9001 tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg
     9061 acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact
     9121 ccggcggcag cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga
     9181 tccaggagag catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg
     9241 agtccgacat cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc
     9301 tgacgagcga cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg
     9361 agaacaagat caagatgctg tccggcggct ccccaaagaa gaagcggaag gtgtaggagc
     9421 tcagagcttt cgttcgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt
     9481 tcattgcgca cacaccagaa tcctactgag tttgagtatt atggcattgg gaaaactgtt
     9541 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc
     9601 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat
     9661 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata
     9721 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc
     9781 gaagttaata tgaggagtaa aacacttgta gttgtaccat tatgcttatt cactaggcaa
     9841 caaatatatt ttcagaccta gaaaagctgc aaatgttact gaatacaagt atgtcctctt
     9901 gtgttttaga catttatgaa ctttccttta tgtaattttc cagaatcctt gtcagattct
     9961 aatcattgct ttataattat agttatactc atggatttgt agttgagtat gaaaatattt
    10021 tttaatgcat tttatgactt gccaattgat tgacaacgaa ttcgtaatca tgtcatagct
    10081 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10141 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10201 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10261 cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac
    10321 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    10381 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    10441 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    10501 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    10561 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10621 tgatgtgata acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    10681 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
    10741 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
    10801 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
    10861 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    10921 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
    10981 gcacaatccc actatccttc gcaagacctt cctctatata aggaagttca tttcatttgg
    11041 agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca
    11101 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    11161 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    11221 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    11281 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    11341 attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg catctcccgc
    11401 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctacaa
    11461 ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    11521 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    11581 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    11641 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    11701 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    11761 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    11821 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    11881 ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg tcttgaccaa
    11941 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    12001 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    12061 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    12121 agcactcgtc cgagggcaaa gaaatagagt agatgccgac cggatctgtc gatcgacaag
    12181 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
    12241 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
    12301 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
    12361 cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
    12421 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
    12481 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
    12541 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
    12601 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
    12661 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
    12721 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
    12781 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
    12841 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
    12901 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
    12961 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
    13021 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
    13081 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
    13141 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
    13201 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
    13261 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
    13321 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
    13381 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
    13441 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
    13501 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
    13561 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
    13621 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
    13681 agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    13741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    13801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    13921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    14041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    14101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    14161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    14221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    14281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    14341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    14401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    14461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    14521 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    14581 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    14641 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
    14701 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    14761 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    14821 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
    14881 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
    14941 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
    15001 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
    15061 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
    15121 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
    15181 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaacaga ccttttcgac
    15241 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
    15301 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
    15361 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
    15421 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
    15481 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
    15541 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
    15601 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
    15661 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
    15721 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
    15781 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
    15841 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
    15901 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
    15961 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
    16021 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
    16081 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
    16141 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
    16201 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
    16261 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
    16321 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
    16381 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
    16441 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
    16501 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
    16561 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
    16621 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
    16681 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
    16741 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
    16801 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
    16861 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
    16921 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
    16981 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
    17041 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
    17101 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
    17161 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
    17221 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
    17281 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
    17341 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
    17401 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
    17461 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
    17521 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
    17581 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
    17641 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
    17701 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    17761 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    17821 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    17881 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    17941 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    18001 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    18061 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    18121 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    18181 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    18241 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    18301 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    18361 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    18421 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    18481 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    18541 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    18601 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    18661 aaccttttca cgccctttta aatatccgtt attctaa
//
