LOCUS       Exported               10489 bp ds-DNA     circular SYN 09-JAN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Ethan Abel
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..410
                     /label=RSV/5' LTR hybrid promoter
                     /label=RSV/5'\LTR\hybrid\promoter
                     /note="/vntifkey=29"
     promoter        1..229
                     /label=RSV promoter
                     /label=RSV\promoter
                     /note="/vntifkey=29"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /label=HIV-1\5'\LTR
                     /note="/vntifkey=19"
     misc_feature    520
                     /label=splice donor
                     /label=splice\donor
                     /label=nonstandard type: splicing_signal
                     /note="/vntifkey=38"
     misc_binding    521..565
                     /label=HIV-1 psi packaging signal
                     /label=HIV-1\psi\packaging\signal
                     /note="/vntifkey=20"
     protein_bind    1075..1308
                     /label=HIV-1 Rev response element
                     /label=HIV-1\Rev\response\element
                     /note="/vntifkey=31"
     misc_feature    1656
                     /label=splice acceptor
                     /label=splice\acceptor
                     /label=nonstandard type: splicing_signal
                     /note="/vntifkey=38"
     misc_feature    1684
                     /label=splice acceptor
                     /label=splice\acceptor
                     /label=nonstandard type: splicing_signal
                     /note="/vntifkey=38"
     misc_RNA        1801..1923
                     /label=cPPT
                     /note="/vntifkey=53"
     promoter        1937..2491
                     /label=CMV/TO promoter
                     /label=CMV/TO\promoter
                     /note="/vntifkey=29"
     promoter        1937..2436
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="/vntifkey=29"
     TATA_signal     2436..2442
                     /label=TATA\box
                     /note="/vntifkey=41"
     protein_bind    2452..2491
                     /label=Tetracycline operator (2X TetO2) sequences
                     /label=Tetracycline\operator\(2X\TetO2)\sequences
                     /note="/vntifkey=31"
     misc_recomb     2531..2555
                     /label=attB1 site
                     /label=attB1\site
                     /note="/vntifkey=86"
     CDS             2576..3292
                     /codon_start=1
                     /label=EGFP coding region
                     /label=EGFP\coding\region
                     /note="/vntifkey=4"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3299..4948
                     /codon_start=1
                     /label=Firefly Luciferase coding region (no stop codon)
                     /label=Firefly\Luciferase\coding\region\(no\stop\codon)
                     /note="/vntifkey=4"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_recomb     4965..4989
                     /label=attB2 site
                     /label=attB2\site
                     /note="/vntifkey=86"
     CDS             5042..5083
                     /codon_start=1
                     /label=V5 epitope
                     /label=V5\epitope
                     /note="/vntifkey=4"
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5051..5071)
                     /label=V5 reverse primer
     CDS             5093..5101
                     /codon_start=1
                     /label=3 stops
                     /label=3\stops
                     /note="/vntifkey=4"
                     /translation="**"
     promoter        5147..5653
                     /label=Human Phosphoglycerate Kinase (hPGK) Promoter
                     /label=Human\Phosphoglycerate\Kinase\(hPGK)\Promoter
                     /note="/vntifkey=29"
     CDS             5675..6274
                     /codon_start=1
                     /label=Puromycin resistance gene
                     /label=Puromycin\resistance\gene
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_RNA        6337..6934
                     /label=WPRE
                     /note="/vntifkey=53"
     LTR             7009..7062
                     /label=deltaU3
                     /note="/vntifkey=19"
     LTR             7010..7243
                     /label=deltaU3/3' LTR
                     /label=deltaU3/3'\LTR
                     /note="/vntifkey=19"
     LTR             7063..7243
                     /label=HIV-1 3' LTR
                     /label=HIV-1\3'\LTR
                     /note="/vntifkey=19"
     polyA_signal    7315..7445
                     /label=SV40 polyadenylation signal
                     /label=SV40\polyadenylation\signal
                     /note="/vntifkey=25"
     primer_bind     complement(7393..7413)
                     /label=SV40 pA reverse primer
     rep_origin      7818..8273
                     /direction=RIGHT
                     /label=f1 origin
                     /label=f1\origin
                     /note="/vntifkey=33"
     promoter        8305..8403
                     /label=bla promoter
                     /label=bla\promoter
                     /note="/vntifkey=30"
     CDS             8404..9264
                     /codon_start=1
                     /label=Amp(R)
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9409..10082
                     /direction=RIGHT
                     /label=pUC origin
                     /label=pUC\origin
                     /note="/vntifkey=33"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatggt
     2581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3181 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccgggat
     3301 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     3361 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     3421 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     3481 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     3541 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     3601 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     3661 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     3721 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     3781 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     3841 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     3901 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     3961 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     4021 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     4081 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     4141 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     4201 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     4261 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     4321 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     4381 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     4441 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     4501 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     4561 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     4621 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     4681 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     4741 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     4801 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     4861 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     4921 ggccaagaag ggcggaaaga tcgccgtgaa gggtgggcgc gccgacccag ctttcttgta
     4981 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     5041 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     5101 agtttggaat taattctgtg gaatgtgtgt cagttagtcg agggggttgg ggttgcgcct
     5161 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     5221 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     5281 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     5341 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     5401 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     5461 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     5521 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     5581 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     5641 cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
     5701 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5761 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6241 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     6301 gaccgaaagg agcgcacgac cccatgcatc ggtacgtcga caatcaacct ctggattaca
     6361 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6421 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6481 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6541 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6601 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6661 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6721 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6781 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6841 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6901 gtcggatctc cctttgggcc gcctccccgc ctggcgatgg tacctttaag accaatgact
     6961 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7021 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7261 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7441 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7501 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7561 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     7621 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     7681 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7741 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7801 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7861 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7921 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7981 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8041 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8101 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8161 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8221 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     8281 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8341 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8401 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8461 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8521 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8581 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8641 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8701 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8761 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8821 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8881 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8941 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9001 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9061 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9121 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9181 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9241 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9301 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9361 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9421 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9481 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9541 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9661 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9781 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9961 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10021 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10081 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10141 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10201 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10261 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10321 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10381 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    10441 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
