LOCUS       Exported                7496 bp ds-DNA     circular SYN 02-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Nordine Helassa
  TITLE     Direct Submission
  JOURNAL   Exported Nov 2, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=M13 origin
     rep_origin      complement(17..457)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(563..1375)
                     /label=KanR
     rep_origin      1475..2103
                     /direction=RIGHT
                     /label=ColE1 origin
     CDS             complement(3281..4237)
                     /label=LacI
     primer_bind     4751..4770
                     /label=T7
     misc_binding    4770..4792
                     /label=LacO
     CDS             4839..5492
                     /label=GST
     CDS             5547..5564
                     /label=Thrombin site
     CDS             5580..5624
                     /label=S-tag
     CDS             5694..7256
                     /codon_start=1
                     /label=iGluSnFR
                     /translation="AAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNA
                     IVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTANNVERQKQAAFSDTIF
                     VVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHGDS
                     FRTLESGRAVAFMMDDVLLAGERAKAKKPDNWEIVGKPQSQEAYGCMLRKDDPQFKKLM
                     DDTIAQVQTSGEAEKWFDKWFKNPILVSHNVYIMADKQRNGIKANFKIRHNIEDGGVQL
                     AYHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY
                     KGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG
                     KLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRA
                     EVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNNPLNMNFELSDEMKALFKEPNDK
                     ALK"
     primer_bind     5938..5972
                     /label=Mutagenic Primer
     primer_bind     6653..6674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6780..6801)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7041..7060)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7324..7341
                     /label=6His
     CDS             7327..7344
                     /label=6His
     CDS             7330..7347
                     /label=6His
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc
     3181 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     3241 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3361 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     3421 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     3481 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     3541 cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     3601 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     3661 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     3721 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     3781 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     3841 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     3901 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     3961 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     4021 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     4081 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     4141 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     4201 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     4261 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     4321 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     4381 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     4441 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     4501 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     4561 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     4621 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     4681 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat
     4741 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     4801 gaaataattt tgtttaactt taagaaggag atatacatat gtcccctata ctaggttatt
     4861 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
     4921 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
     4981 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
     5041 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
     5101 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
     5161 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
     5221 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
     5281 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
     5341 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     5401 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     5461 ccacgtttgg tggtggcgac catcctccaa aatcggatgg ttcaactagt ggttctggtc
     5521 atcaccatca ccatcactcc gcgggtctgg tgccacgcgg tagtactgca attggtatga
     5581 aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga cagcccagat ctgggtaccg
     5641 gtggtggctc cggtgatgac gacgacaaga gtcccatggg atatcgggga tccgccgcag
     5701 gcagcacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt
     5761 cagtgccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg caggattact
     5821 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac
     5881 tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
     5941 aatgtggttc taccgccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
     6001 ttttcgtggt cggtacgcgc ctgttgacca aaaagggtgg cgatatcaaa gattttgcca
     6061 acctgaaaga caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
     6121 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
     6181 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gtgctgctgg
     6241 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc aagccgcagt
     6301 ctcaggaggc ctacggttgt atgttgcgta aagatgatcc gcagttcaaa aagctgatgg
     6361 atgacaccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataagtggt
     6421 tcaaaaatcc aattctggtg agccacaacg tctatatcat ggccgacaag cagaggaacg
     6481 gcatcaaggc gaacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgcct
     6541 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6601 acctgagcac ccagtccaaa ctttcgaaag accccaacga gaagcgcgat cacatggtcc
     6661 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg
     6721 gtaccggagg gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6781 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggtgagg
     6841 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6901 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6961 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacatccagg
     7021 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7081 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7141 acatcctggg gcataagctg gagtacaact ttaacaaccc cctgaacatg aatttcgaac
     7201 tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg aaataagaat
     7261 tctgtacagg ccttggcgcg cctgcaggcg agctccgtcg acaagcttgc ggccgcactc
     7321 gagcaccacc accaccacca ccaccactaa ttgattaata cctaggctgc taaacaaagc
     7381 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     7441 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat
//
