LOCUS       Cmv_d0_dPspCas13b-GGS-N 7086 bp ds-DNA     circular     26-APR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6750..7035
                     /label="Cer region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      6043..6079
                     /label="ColE1-derived rep origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            5080..5874
                     /label="kanR gene"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1433..1450
                     /label="HEPN1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             4001..4054
                     /label="GGS"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     misc_feature    1052..4000
                     /label="dPspCas13b delta 984-1090"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4551..4772
                     /label="SV40 late polyadenylation signal"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1..742
                     /label="CMV immediate/early promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4055..4357
                     /label="N-Y1(100-200)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5080..5874
                     /label="kanR CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     intron          857..989
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggcAGATCA CTAGAAGCTT ACGCTACCAC Catgaacatc cccgctctgg tggaaaacca
     1081 gaagaagtac tttggcacct acagcgtgat ggccatgctg aacgctcaga ccgtgctgga
     1141 ccacatccag aaggtggccg atattgaggg cgagcagaac gagaacaacg agaatctgtg
     1201 gtttcacccc gtgatgagcc acctgtacaa cgccaagaac ggctacgaca agcagcccga
     1261 gaaaaccatg ttcatcatcg agcggctgca gagctacttc ccattcctga agatcatggc
     1321 cgagaaccag agagagtaca gcaacggcaa gtacaagcag aaccgcgtgg aagtgaacag
     1381 caacgacatc ttcgaggtgc tgaagcgcgc cttcggcgtg ctgaagatgt acagggacct
     1441 gaccaacGCA tacaagacct acgaggaaaa gctgaacgac ggctgcgagt tcctgaccag
     1501 cacagagcaa cctctgagcg gcatgatcaa caactactac acagtggccc tgcggaacat
     1561 gaacgagaga tacggctaca agacagagga cctggccttc atccaggaca agcggttcaa
     1621 gttcgtgaag gacgcctacg gcaagaaaaa gtcccaagtg aataccggat tcttcctgag
     1681 cctgcaggac tacaacggcg acacacagaa gaagctgcac ctgagcggag tgggaatcgc
     1741 cctgctgatc tgcctgttcc tggacaagca gtacatcaac atctttctga gcaggctgcc
     1801 catcttctcc agctacaatg cccagagcga ggaacggcgg atcatcatca gatccttcgg
     1861 catcaacagc atcaagctgc ccaaggaccg gatccacagc gagaagtcca acaagagcgt
     1921 ggccatggat atgctcaacg aagtgaagcg gtgccccgac gagctgttca caacactgtc
     1981 tgccgagaag cagtcccggt tcagaatcat cagcgacgac cacaatgaag tgctgatgaa
     2041 gcggagcagc gacagattcg tgcctctgct gctgcagtat atcgattacg gcaagctgtt
     2101 cgaccacatc aggttccacg tgaacatggg caagctgaga tacctgctga aggccgacaa
     2161 gacctgcatc gacggccaga ccagagtcag agtgatcgag cagcccctga acggcttcgg
     2221 cagactggaa gaggccgaga caatgcggaa gcaagagaac ggcaccttcg gcaacagcgg
     2281 catccggatc agagacttcg agaacatgaa gcgggacgac gccaatcctg ccaactatcc
     2341 ctacatcgtg gacacctaca cacactacat cctggaaaac aacaaggtcg agatgtttat
     2401 caacgacaaa gaggacagcg ccccactgct gcccgtgatc gaggatgata gatacgtggt
     2461 caagacaatc cccagctgcc ggatgagcac cctggaaatt ccagccatgg ccttccacat
     2521 gtttctgttc ggcagcaaga aaaccgagaa gctgatcgtg gacgtgcaca accggtacaa
     2581 gagactgttc caggccatgc agaaagaaga agtgaccgcc gagaatatcg ccagcttcgg
     2641 aatcgccgag agcgacctgc ctcagaagat cctggatctg atcagcggca atgcccacgg
     2701 caaggatgtg gacgccttca tcagactgac cgtggacgac atgctgaccg acaccgagcg
     2761 gagaatcaag agattcaagg acgaccggaa gtccattcgg agcgccgaca acaagatggg
     2821 aaagagaggc ttcaagcaga tctccacagg caagctggcc gacttcctgg ccaaggacat
     2881 cgtgctgttt cagcccagcg tgaacgatgg cgagaacaag atcaccggcc tgaactaccg
     2941 gatcatgcag agcgccattg ccgtgtacga tagcggcgac gattacgagg ccaagcagca
     3001 gttcaagctg atgttcgaga aggcccggct gatcggcaag ggcacaacag agcctcatcc
     3061 atttctgtac aaggtgttcg cccgcagcat ccccgccaat gccgtcgagt tctacgagcg
     3121 ctacctgatc gagcggaagt tctacctgac cggcctgtcc aacgagatca agaaaggcaa
     3181 cagagtggat gtgcccttca tccggcggga ccagaacaag tggaaaacac ccgccatgaa
     3241 gaccctgggc agaatctaca gcgaggatct gcccgtggaa ctgcccagac agatgttcga
     3301 caatgagatc aagtcccacc tgaagtccct gccacagatg gaaggcatcg acttcaacaa
     3361 tgccaacgtg acctatctga tcgccgagta catgaagaga gtgctggacg acgacttcca
     3421 gaccttctac cagtggaacc gcaactaccg gtacatggac atgcttaagg gcgagtacga
     3481 cagaaagggc tccctgcagc actgcttcac cagcgtggaa gagagagaag gcctctggaa
     3541 agagcgggcc tccagaacag agcggtacag aaagcaggcc agcaacaaga tccgcagcaa
     3601 ccggcagatg agaaacgcca gcagcgaaga gatcgagaca atcctggata agcggctgag
     3661 caacagccgg aacgagtacc agaaaagcga gaaagtgatc cggcgctaca gagtgcagga
     3721 tgccctgctg tttctgctgg ccaaaaagac cctgaccgaa ctggccgatt tcgacggcga
     3781 gaggttcaaa ctgaaagaaa tcatgcccga cgccgagaag ggaatcctga gcgagatcat
     3841 gcccatgagc ttcaccttcg agaaaggcgg caagaagtac accatcacca gcgagggcat
     3901 gaagctgaag aactacggcg acttctttgt gctggctagc gacaagagga tcggcaacct
     3961 gctggaactc gtgggcagcg acatcgtgtc caaagaggat GGCGGTAGTG GAGGAAGCGG
     4021 GGGGAGCGGA GGGAGTGGTG GATCCGGCGG CTCCATGCAC GATGCTGTTT TTGGGCAGCC
     4081 TGGGGGCCTG GGGAACAACA TCTATCAGCA CAGGTTCAAT TTTTTCCCTG AAAACCCTGC
     4141 GTTCTCAGCA TGGGGGACAA GTGGGTCTCA AGGTCAGCAG ACCCAGAGCT CCGCGTATGG
     4201 GAGCAGCTAC ACCTACCCCC CGAGCTCCCT GGGTGGCACG GTGGTTGATG GGCAGCCAGG
     4261 CTTTCACAGC GACACCCTCA GCAAGGCCCC CGGGATGAAC AGCCTGGAGC AGGGCATGGT
     4321 TGGCCTGAAG ATTGGGGACG TCAGCTCCTC CGCCGTCgga tccGATTACA AGGACGACGA
     4381 TGACAAGTAG tgtttaaacg aattcgggct cggtacacgg ggatcctcta gagtcgacct
     4441 gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     4501 gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat
     4561 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4621 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4681 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4741 tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttctaatgga tcctctttgc
     4801 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4861 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     4921 cttgcggcag cgtgagcttc aaaagaattg ccagctgggg cgccctctgg taaggttggg
     4981 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     5041 tcaagatctg atcaagagac aggatgacgg tcgtttcgca tgcttgaaca agatggattg
     5101 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5161 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5221 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5281 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5341 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5401 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5461 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgcactcgg
     5521 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5581 gccgaactgt tcgccaggct caaggcgcgt atgccggatg gtgaggatct cgtcgtgact
     5641 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5701 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5761 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5821 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5881 ctctggggtt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc
     5941 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6001 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6061 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6481 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6601 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     6661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6721 aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc
     6781 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     6841 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt
     6901 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     6961 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     7021 tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg
     7081 agatct
//