LOCUS       hU6_promoter_PspCas13b_ 3217 bp ds-DNA     circular     26-APR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        600..616
                     /label="M13_forward20_primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    585..607
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1081..1103)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(459..614)
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    909..909
                     /label="G"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    996..1002
                     /label="terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    910..959
                     /label="target"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    960..995
                     /label="PspCas13b DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(2229..3089)
                     /label="ORF frame 3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            complement(2229..3089)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(3131..3159)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        626..644
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(135..441)
                     /label="f1_origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1028..1047)
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(1117..1146)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        668..908
                     /label="hU6_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(1064..1082)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacCGA
      661 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT
      721 AATTGGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA
      781 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT
      841 GCTTAtCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
      901 AAACACCGGC CTGCAGGTCG ACTCTAGACT CGAGGCTAGC GAGCTCGTTT AAACAACTAg
      961 ttgtggaagg tccagttttg aggggctatt acaacTTTTT TTCCgcggtg gagctccagc
     1021 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     1081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3181 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//