LOCUS       Exported                5697 bp ds-DNA     linear   SYN 03-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2018 from SnapGene Viewer 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..28
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="color: #1fa310"
     primer_bind     17..38
                     /label=#5 seq for 1
                     /note="color: black; sequence: CGACTCACTATAGGGCGAATTG"
     misc_feature    89..140
                     /label=3 X HA tag
                     /note="3 X HA tag"
                     /note="color: #58fff3"
     misc_feature    102..140
                     /label=G1408a primer
                     /note="G1408a primer"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    114..128
                     /label=G1408a
                     /note="G1408a"
                     /note="color: #fff74b"
     misc_feature    128..1618
                     /label=sequence verified
                     /note="sequence verified"
                     /note="color: #c0c0c0"
     primer_bind     192..214
                     /label=Primer 3
                     /note="color: black; sequence: 
                     -------50bp-------ATGGGCTGCATCAAGAGCAAGC"
     misc_feature    193..294
                     /label=2xMembraneLocalization
                     /note="color: #ff1700"
     primer_bind     complement(232..249)
                     /label=2x myr rev
                     /note="color: black; sequence: CATGGCGGCCTCGTCGTC"
     primer_bind     242..268
                     /label=Primer 3
                     /note="color: black; sequence: 
                     -------50bp-------ATGGGCTGCATCAAGAGCAAGC"
     primer_bind     complement(286..295)
                     /label=2x myr rev
                     /note="color: black; sequence: CATGGCGGCCTCGTCGTC"
     misc_feature    295..306
                     /label=GGSG linker
                     /note="GGSG linker"
                     /note="color: #ffffff"
     primer_bind     305..327
                     /label=myr Cherry/Cit for
                     /note="color: black; sequence: GATCTGTGAGCAAGGGCGAGGAG"
     misc_feature    310..1023
                     /label=Citrine
                     /note="Citrine"
                     /note="color: #13ffb3; direction: RIGHT"
     primer_bind     428..447
                     /label=#5 seq for 2
                     /note="color: black; sequence: GCAAGCTGACCCTGAAGTTC"
     misc_feature    592..623
                     /label=gagcgcaccatcttcttcaaAgacgacggcaa
                     /note="gagcgcaccatcttcttcaaAgacgacggcaa"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    complement(592..623)
                     /label=ttgccgtcgtcTttgaagaagatggtgcgctc
                     /note="ttgccgtcgtcTttgaagaagatggtgcgctc"
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    612
                     /label=Untitled
                     /note="color: #0080ff"
     primer_bind     685..704
                     /label=AcDs seq10
                     /note="color: black; sequence: AAGGGCATCGACTTCAAGGA"
     primer_bind     848..867
                     /label=#5 seq for 3
                     /note="color: black; sequence: ACCACTACCAGCAGAACACC"
     primer_bind     complement(932..951)
                     /label=Primer 11
                     /note="color: black; sequence: CTTCTCGTTGGGGTCTTTGC"
     primer_bind     complement(1004..1025)
                     /label=Primer 1
                     /note="color: black; sequence: TTTA CTTGTACAGCTCGTCCATGC"
     misc_feature    1069..1290
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
                     /note="color: #ebfeed"
     primer_bind     complement(1104..1124)
                     /label=Dendra2 mut seq rev
                     /note="color: black; sequence: TCACTGCATTCTAGTTGTGGT"
     misc_feature    1212..1247
                     /label=SA mutated sense A49g
                     /note="SA mutated sense A49g"
                     /note="color: #4cff23; direction: RIGHT"
     misc_feature    complement(1212..1247)
                     /label=SA mutated antisense A49g
                     /note="SA mutated antisense A49g"
                     /note="color: #9324ff; direction: LEFT"
     misc_feature    1212..1232
                     /label=SA strong
                     /note="SA strong"
                     /note="color: #0080ff"
     misc_feature    1231
                     /label=Untitled
                     /note="color: #0080ff"
     primer_bind     1240..1258
                     /label=Primer 7
                     /note="color: black; sequence: GGGAGGTGTGGGAGGTTTT"
     misc_feature    complement(1323..1370)
                     /label=FRT
                     /note="FRT"
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     1743..1763
                     /label=Primer 8
                     /note="color: black; sequence: AGTCAGTAACCATAGTCCGCA"
     misc_feature    1800..2594
                     /label=Kan
                     /note="Kan"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    1836..3191
                     /label=sequence verified
                     /note="sequence verified"
                     /note="color: #c0c0c0"
     primer_bind     2169..2188
                     /label=Primer 9
                     /note="color: black; sequence: CATACGCTTGATCCGGCTAC"
     primer_bind     2570..2589
                     /label=Primer 10
                     /note="color: black; sequence: TCGCCTTCTTGACGAGTTCT"
     misc_feature    complement(2674..2721)
                     /label=FRT
                     /note="FRT"
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     complement(2740..2768)
                     /label=Primer 2
                     /note="color: black; sequence: -----50bp------ 
                     TCCAGAAGTAGTGAGGAGGCTTT"
     misc_feature    complement(2749..2763)
                     /label=rev Kan recomb primer
                     /note="rev Kan recomb primer"
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    4047..4907
                     /label=Ampr
                     /note="Ampr"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    5090..5545
                     /label=phage f1 region
                     /note="phage f1 region"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    join(5546..5697,1..9)
                     /label=Lac operon sequences
                     /note="Lac operon sequences"
                     /note="color: #800080; direction: RIGHT"
     misc_feature    5666..5682
                     /label=pUC M13 for
                     /note="pUC M13 for"
                     /note="color: #0000ff"
ORIGIN
        1 ccagtgaatt gtaatacgac tcactatagg gcgaattggg cccgacgtcg catgctcccg
       61 gccgccatgg cggccgcgga tggccaccta tgacgtccca gattacgcaa gtttgcccgg
      121 atatgatgtt cctgattatg ctagcctccc gggttacgat gtgcccgact atgcctcact
      181 tccaggcaga tcatgggctg catcaagagc aagcgcaagg acaacctgaa cgacgacgag
      241 gccgccatgg gctgcatcaa gagcaagcgc aaggacaacc tgaacgacga cgagggtgga
      301 agcggatctg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      361 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      421 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      481 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      541 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      601 atcttcttca aagacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      661 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      721 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      781 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      841 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      901 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      961 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1021 aagtaaagcg gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1081 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1141 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1201 acaacaacaa ttgcattcat tttatgtttc gggttcaggg ggaggtgtgg gaggtttttt
     1261 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgacctgc
     1321 ttgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aagatccccc
     1381 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcgtcag
     1441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1561 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     1621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     1681 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1741 ttagtcagta accatagtcc gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     1801 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1861 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1981 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     2041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     2101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     2161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     2221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     2281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     2341 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     2401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     2461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     2521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     2581 acgagttctt ctgaggggat cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     2641 cgtttgttcg gatccgagct tcaaaagcgc tctgaagttc ctatactttc tagagaatag
     2701 gaacttcgga atagtaactt ctccatggta gcctccaaaa aagcctcctc actacttctg
     2761 gaatagctca atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc
     2821 ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa
     2881 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2941 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3001 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3061 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4201 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat
     5041 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     5101 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     5161 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     5221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     5281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     5341 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     5401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     5461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5521 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     5581 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5641 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacgg
//
