LOCUS       Exported                6408 bp ds-DNA     circular SYN 03-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2018 from SnapGene Viewer 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=phage f1 region
                     /note="phage f1 region"
                     /note="color: #ff8000"
     misc_feature    457..617
                     /label=lac operon sequences
                     /note="lac operon sequences"
                     /note="color: #ff0080; direction: RIGHT"
     primer_bind     577..593
                     /label=pUC/M13 Forward Sequencing Primer
                     /note="pUC/M13 Forward Sequencing Primer binding site"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    620..636
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="color: #1fa310"
     misc_feature    697..748
                     /label=3 X HA tag
                     /note="3 X HA tag"
                     /note="color: #58fff3"
     misc_feature    710..748
                     /label=G1408a primer
                     /note="G1408a primer"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    722..736
                     /label=G1408a
                     /note="G1408a"
                     /note="color: #fff74b"
     primer_bind     781..865
                     /label=GCCTCACTTCCAGGCAGATCATGGGCTGCATCAAGAGCAAGCGCAAGGAC
                     /note="color: black; sequence: 
                     GCCTCACTTCCAGGCAGATCATGGGCTGCATCAAGAGCAAGCGCAAGGACAACCTGAAC
                     GACGACGAGGCCGCCATGGGCTGCAT"
     primer_bind     800..826
                     /label=5' recomb myr - ---------50bp----------ATGGGCTGCAT
                     /note="color: black; sequence: 
                     ---------50bp----------ATGGGCTGCATCAAGAGCAAGCGCAA"
     misc_feature    801..902
                     /label=2xMembraneLocalization
                     /note="color: #ff1700"
     primer_bind     complement(846..932)
                     /label=CTCGCCCTTGCTCACAGATCCGCTTCCACCCTCGTCGTCGTTCAGGTTGT
                     /note="color: black; sequence: 
                     CTCGCCCTTGCTCACAGATCCGCTTCCACCCTCGTCGTCGTTCAGGTTGTCCTTGCGCT
                     TGCTCTTGATGCAGCCCATGGCGGCCTC"
     primer_bind     850..880
                     /label=5' recomb myr - ---------50bp----------ATGGGCTGCAT
                     /note="color: black; sequence: 
                     ---------50bp----------ATGGGCTGCATCAAGAGCAAGCGCAA"
     misc_feature    903..914
                     /label=GGSG linker
                     /note="GGSG linker"
                     /note="color: #ffffff"
     primer_bind     913..946
                     /label=Primer 8
                     /note="color: black; sequence: 
                     GATCTGTGAGCAAGGGCGAGGAGGTCATCAAAGA"
     misc_feature    918..2342
                     /label=tdTomato ORF
                     /note="tdTomato ORF"
                     /note="color: #1611ff"
     primer_bind     complement(2336..2355)
                     /label=CGCGGCCGCTTTACTTGTAC
                     /note="color: black; sequence: CGCGGCCGCTTTACTTGTAC"
     primer_bind     complement(2338..2359)
                     /label=Primer 7
                     /note="color: black; sequence: GAGTCGCGGCCGCTTTACTTGT"
     misc_feature    2388..2609
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
                     /note="color: #ebfeed"
     misc_feature    complement(2531..2566)
                     /label=SA mutated antisense A49g
                     /note="SA mutated antisense A49g"
                     /note="color: #9324ff; direction: LEFT"
     misc_feature    2531..2566
                     /label=SA mutated sense A49g
                     /note="SA mutated sense A49g"
                     /note="color: #4cff23; direction: RIGHT"
     misc_feature    2531..2551
                     /label=SA strong
                     /note="SA strong"
                     /note="color: #0080ff"
     misc_feature    2550
                     /label=Untitled
                     /note="color: #0080ff"
     misc_feature    complement(2642..2689)
                     /label=FRT
                     /note="FRT"
                     /note="color: #ffff00; direction: LEFT"
     misc_feature    3119..3913
                     /label=Kan
                     /note="Kan"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    complement(3993..4040)
                     /label=FRT
                     /note="FRT"
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     complement(4059..4087)
                     /label=recombination BirA 3' - -----50bp------ TCCAG
                     /note="color: black; sequence: -----50bp------ 
                     TCCAGAAGTAGTGAGGAGGCTTT"
     misc_feature    complement(4068..4082)
                     /label=rev Kan recomb primer
                     /note="rev Kan recomb primer"
                     /note="color: #0000ff; direction: LEFT"
     promoter        4168..4187
                     /label=SP6 RNA polymerase promoter (-17 to +3)
                     /note="SP6 RNA polymerase promoter (-17 to +3)"
                     /note="color: #008000"
     misc_feature    4170
                     /label=SP6 RNA polymerase transcription
                     /note="SP6 RNA polymerase transcription initiation site"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    4195..4424
                     /label=lac operon sequences
                     /note="lac operon sequences"
                     /note="color: #ff0080; direction: RIGHT"
     primer_bind     4205..4221
                     /label=pUC/M13 Reverse Sequencing Primer
                     /note="pUC/M13 Reverse Sequencing Primer binding site"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    4209..4211
                     /label=lacZ start codon
                     /note="lacZ start codon"
                     /note="color: #0000ff"
     misc_feature    4229..4245
                     /label=lac operator
                     /note="lac operator"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             5366..6226
                     /label=Ampr
                     /note="beta-lactamase (Ampr) coding region"
                     /note="color: #0000ff; direction: RIGHT"
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca
      661 tgctcccggc cgccatggcg gccgcggatg gccacctatg acgtcccaga ttacgcaagt
      721 ttgcccggat atgatgttcc tgattatgct agcctcccgg gttacgatgt gcccgactat
      781 gcctcacttc caggcagatc atgggctgca tcaagagcaa gcgcaaggac aacctgaacg
      841 acgacgaggc cgccatgggc tgcatcaaga gcaagcgcaa ggacaacctg aacgacgacg
      901 agggtggaag cggatctgtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
      961 aagttcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     1021 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     1081 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     1141 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     1201 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     1261 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     1321 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     1381 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     1441 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     1501 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1561 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
     1621 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     1681 gcttcaaagt tcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     1741 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     1801 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     1861 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     1921 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     1981 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     2041 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     2101 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     2161 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     2221 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     2281 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     2341 agtaaagcgg ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag
     2401 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2461 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2521 caacaacaat tgcattcatt ttatgtttcg ggttcagggg gaggtgtggg aggtttttta
     2581 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgacctgct
     2641 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca agatccccct
     2701 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcgtcagg
     2761 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2821 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2881 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     2941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3001 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3061 tagtcagtaa ccatagtccg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3121 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3181 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3241 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3301 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3361 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3421 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3481 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3541 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3601 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     3661 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     3721 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3781 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3841 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3901 cgagttcttc tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     3961 gtttgttcgg atccgagctt caaaagcgct ctgaagttcc tatactttct agagaatagg
     4021 aacttcggaa tagtaacttc tccatggtag cctccaaaaa agcctcctca ctacttctgg
     4081 aatagctcaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc
     4141 caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat
     4201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4381 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg
     6361 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcagg
//
