LOCUS       addgene_plasmid_        6157 bp ds-DNA     circular     06-NOV-2018
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    pET28GST-LIC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7994)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2018 from SnapGene 4.2.1 http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    951..2141
                     /locus_tag="ARC (match accession XP_008763812)"
                     /label="ARC (match accession XP_008763812)"
                     /ApEinfo_label="ARC (match accession XP_008763812)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    258..950
                     /locus_tag="GST"
                     /label="GST"
                     /ApEinfo_label="GST"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    184..212
                     /locus_tag="tac promoter"
                     /label="tac promoter"
                     /ApEinfo_label="tac promoter"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..938
                     /locus_tag="Prescission protease site"
                     /label="Prescission protease site"
                     /ApEinfo_label="Prescission protease site"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2565..3423
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
       61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
      121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
      181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
      301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
      361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
      421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
      481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
      541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
      601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
      661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
      721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
      781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
      841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
      901 ATCCTCCAAA ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC ATGGAGCTGG
      961 ACCATATGAC GACCGGCGGC CTCCACGCCT ACCCTGCCCC GCGGGGTGGG CCGGCCGCCA
     1021 AACCCAATGT GATCCTGCAG ATTGGTAAGT GCCGAGCTGA GATGCTGGAG CACGTACGGA
     1081 GGACCCACCG GCATCTGTTG ACCGAAGTGT CCAAGCAGGT GGAGCGAGAG CTGAAAGGGT
     1141 TGCACAGGTC GGTGGGCAAG CTGGAGAACA ACTTGGACGG CTATGTGCCC ACGGGCGACT
     1201 CACAGCGCTG GAAGAAGTCC ATCAAGGCCT GTCTCTGCCG CTGCCAGGAG ACCATCGCCA
     1261 ACCTGGAGCG CTGGGTCAAG CGTGAGATGC ACGTGTGGAG GGAGGTCTTC TACCGTCTGG
     1321 AGAGGTGGGC CGACCGCCTG GAGTCCATGG GCGGCAAGTA CCCAGTGGGC AGCGAGCCGG
     1381 CCCGCCACAC TGTCTCTGTA GGTGTGGGGG GTCCAGAGCC CTACTGCCAG GAAGCTGATG
     1441 GCTACGACTA CACTGTTAGC CCCTATGCCA TCACCCCGCC ACCTGCCGCA GGAGAGCTGC
     1501 CTGAGCAGGA GTCAGTTGGG GCTCAGCAAT ACCAGTCTTG GGTGCCAGGT GAGGATGGGC
     1561 AACCAAGCCC AGGTGTGGAT ACCCAGATCT TTGAGGACCC ACGGGAGTTC CTGAGCCACC
     1621 TGGAAGAGTA CCTGCGGCAG GTGGGTGGCT CTGAAGAATA TTGGCTGTCC CAGATCCAGA
     1681 ACCACATGAA TGGGCCAGCC AAGAAGTGGT GGGAGTTCAA ACAGGGCTCG GTGAAGAACT
     1741 GGGTGGAGTT CAAGAAGGAG TTTCTGCAGT ACAGTGAGGG TACGCTCTCC CGCGAAGCCA
     1801 TTCAGCGGGA GCTGGACCTG CCACAGAAGC AGGGTGAGCC ACTTGACCAG TTCCTCTGGC
     1861 GTAAGCGGGA CCTGTACCAG ACACTGTATG TGGACGCTGA GGAGGAGGAG ATCATTCAGT
     1921 ATGTGGTGGG CACCCTGCAG CCCAAGTTCA AGCGCTTTCT GCGCCACCCA CTTCCCAAGA
     1981 CCCTGGAGCA GCTCATCCAG AGGGGCATGG AAGTTCAGGA CGGCCTGGAG CAGGCAGCTG
     2041 AGCCTTCTGT CACCCCTCTG CCCACAGAGG ATGAGACTGA GGCACTCACG CCTGCTCTTA
     2101 CCAGCGAGTC AGTAGCCAGT GACAGGACCC AGCCTGAATA GCTCGAGCGG CCGCATCGTG
     2161 ACTGACTGAC GATCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC
     2221 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
     2281 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG
     2341 ATAGCGGAGT GTATAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG
     2401 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC
     2461 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
     2521 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT
     2581 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG
     2641 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG
     2701 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA
     2761 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC
     2821 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG
     2881 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA
     2941 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC
     3001 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG
     3061 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA
     3121 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA
     3181 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG
     3241 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG
     3301 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG
     3361 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
     3421 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
     3481 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
     3541 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     3601 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     3661 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     3721 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     3781 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     3841 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     3901 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     3961 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     4021 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     4081 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     4141 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     4201 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     4261 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA
     4321 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT
     4381 ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT
     4441 GGTGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG
     4501 TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA
     4561 CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG
     4621 AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA
     4681 AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA
     4741 TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG
     4801 TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG
     4861 TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG
     4921 CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA
     4981 TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC
     5041 ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG
     5101 CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG
     5161 ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC
     5221 CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG
     5281 AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA
     5341 GCTCATGTTA TATCCCGCCG TCAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA
     5401 CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT
     5461 TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC
     5521 CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG
     5581 GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA
     5641 CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA
     5701 GGAAACAGCT ATGACCATGA TTACGGATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG
     5761 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG
     5821 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
     5881 CGAATGGCGC TTTGCCTGGT TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG
     5941 CGATCTTCCT GAGGCCGATA CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA
     6001 TGCGCCCATC TACACCAACG TAACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC
     6061 GGAGAATCCG ACGGGTTGTT ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA
     6121 AGGCCAGACG CGAATTATTT TTGATGGCGT TGGAATT  
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