LOCUS       pGEX_6p1_Arc_FL         5878 bp ds-DNA     circular     06-NOV-2018
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    pET28GST-LIC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7994)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2018 from SnapGene 4.2.1 http://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    951..1862
                     /locus_tag="ARC (match accession XP_008763812)"
                     /label="ARC (match accession XP_008763812)"
                     /ApEinfo_label="ARC (match accession XP_008763812)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    258..950
                     /locus_tag="GST"
                     /label="GST"
                     /ApEinfo_label="GST"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..941
                     /locus_tag="PreCission Protease Site"
                     /label="PreCission Protease Site"
                     /ApEinfo_label="PreCission Protease Site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
       61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
      121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
      181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
      241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
      301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
      361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
      421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
      481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
      541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
      601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
      661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
      721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
      781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
      841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
      901 ATCCTCCAAA ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC ATGGAGCTGG
      961 ACCATATGAC GACCGGCGGC CTCCACGCCT ACCCTGCCCC GCGGGGTGGG CCGGCCGCCA
     1021 AACCCAATGT GATCCTGCAG ATTGGTAAGT GCCGAGCTGA GATGCTGGAG CACGTACGGA
     1081 GGACCCACCG GCATCTGTTG ACCGAAGTGT CCAAGCAGGT GGAGCGAGAG CTGAAAGGGT
     1141 TGCACAGGTC GGTGGGCAAG CTGGAGAACA ACTTGGACGG CTATGTGCCC ACGGGCGACT
     1201 CACAGCGCTG GAAGAAGTCC ATCAAGGCCT GTCTCTGCCG CTGCCAGGAG ACCATCGCCA
     1261 ACCTGGAGCG CTGGGTCAAG CGTGAGATGC ACGTGTGGAG GGAGGTCTTC TACCGTCTGG
     1321 AGAGGTGGGC CGACCGCCTG GAGTCCATGG GCGGCAAGTA CCCAGTGGGC AGCGAGCCGG
     1381 CCCGCCACAC TGTCTCTGTA GGTGTGGGGG GTCCAGAGCC CTACTGCCAG GAAGCTGATG
     1441 GCTACGACTA CACTGTTAGC CCCTATGCCA TCACCCCGCC ACCTGCCGCA GGAGAGCTGC
     1501 CTGAGCAGGA GTCAGTTGGG GCTCAGCAAT ACCAGTCTTG GGTGCCAGGT GAGGATGGGC
     1561 AACCAAGCCC AGGTGTGGAT ACCCAGATCT TTGAGGACCC ACGGGAGTTC CTGAGCCACC
     1621 TGGAAGAGTA CCTGCGGCAG GTGGGTGGCT CTGAAGAATA TTGGCTGTCC CAGATCCAGA
     1681 ACCACATGAA TGGGCCAGCC AAGAAGTGGT GGGAGTTCAA ACAGGGCTCG GTGAAGAACT
     1741 GGGTGGAGTT CAAGAAGGAG TTTCTGCAGT ACAGTGAGGG TCCCACAGAG GATGAGACTG
     1801 AGGCACTCAC GCCTGCTCTT ACCAGCGAGT CAGTAGCCAG TGACAGGACC CAGCCTGAAT
     1861 AGCTCGAGCG GCCGCATCGT GACTGACTGA CGATCTGCCT CGCGCGTTTC GGTGATGACG
     1921 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
     1981 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG
     2041 CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATAATTC TTGAAGACGA AAGGGCCTCG
     2101 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
     2161 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     2221 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
     2281 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
     2341 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
     2401 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
     2461 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
     2521 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
     2581 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
     2641 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
     2701 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
     2761 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
     2821 CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
     2881 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
     2941 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
     3001 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
     3061 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
     3121 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
     3181 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     3241 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     3301 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     3361 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     3421 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
     3481 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     3541 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     3601 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     3661 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     3721 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     3781 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     3841 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     3901 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     3961 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
     4021 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
     4081 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
     4141 TAAATTCCGA CACCATCGAA TGGTGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG
     4201 AAGAGAGTCA ATTCAGGGTG GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT
     4261 ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG
     4321 CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG
     4381 TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG
     4441 CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG
     4501 CCAGCGTGGT GGTGTCGATG GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC
     4561 ACAATCTTCT CGCGCAACGC GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG
     4621 ATGCCATTGC TGTGGAAGCT GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG
     4681 ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA CGGTACGCGA CTGGGCGTGG
     4741 AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG
     4801 TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC
     4861 CGATAGCGGA ACGGGAAGGC GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCAAA
     4921 TGCTGAATGA GGGCATCGTT CCCACTGCGA TGCTGGTTGC CAACGATCAG ATGGCGCTGG
     4981 GCGCAATGCG CGCCATTACC GAGTCCGGGC TGCGCGTTGG TGCGGATATC TCGGTAGTGG
     5041 GATACGACGA TACCGAAGAC AGCTCATGTT ATATCCCGCC GTCAACCACC ATCAAACAGG
     5101 ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG ACCGCTTGCT GCAACTCTCT CAGGGCCAGG
     5161 CGGTGAAGGG CAATCAGCTG TTGCCCGTCT CACTGGTGAA AAGAAAAACC ACCCTGGCGC
     5221 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC
     5281 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT
     5341 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG
     5401 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGGATT CACTGGCCGT
     5461 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     5521 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
     5581 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT
     5641 GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA
     5701 CTGGCAGATG CACGGTTACG ATGCGCCCAT CTACACCAAC GTAACCTATC CCATTACGGT
     5761 CAATCCGCCG TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA CATTTAATGT
     5821 TGATGAAAGC TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG TTGGAATT
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