LOCUS       pDD120                  6247 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12540758|
COMMENT     VNTDBDATE|-12540758|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDD120|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    2433..3431
                     /ApEinfo_fwdcolor=#ff6fcf
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=KanR\cassette
     rep_origin      111..730
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(994..1239)
                     /codon_start=1
                     /transl_table=11
                     /gene="repB"
                     /locus_tag="pB264_p1"
                     /product="putative theta replicase"
                     /db_xref="GI:40458419"
                     /db_xref="GeneID:2699953"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="NP_954861.1"
                     /vntifkey="4"
                     /label=repB
                     /note="required for plasmid replication; may be supplied in trans"
     CDS             complement(1232..2122)
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /locus_tag="pB264_p2"
                     /product="putative theta replicase"
                     /db_xref="GI:32128474"
                     /db_xref="GeneID:1094391"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="NP_858932.1"
                     /vntifkey="4"
                     /label=repA
                     /note="required for plasmid replication; may be supplied in trans"
     repeat_region   1504..1517
                     /rpt_family="IR3"
                     /vntifkey="34"
                     /label=repeat\region
     repeat_region   complement(1678..1691)
                     /gene="repA"
                     /locus_tag="pB264_p2"
                     /rpt_family="IR3"
                     /vntifkey="34"
                     /label=repeat\region
     repeat_region   2207..2255
                     /rpt_family="DR2"
                     /vntifkey="34"
                     /label=repeat\region
     repeat_region   806..819
                     /rpt_family="IR2"
                     /vntifkey="34"
                     /label=repeat\region
     repeat_region   869..882
                     /rpt_family="IR4"
                     /vntifkey="34"
                     /label=repeat\region
     misc_feature    3977..4049
                     /vntifkey="21"
                     /label=UTR\+\RBS
     misc_feature    3965..3970
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-10\site
     misc_feature    3942..3945
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-35\site
     misc_feature    3936..3976
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pConstitutive
     terminator      3754..3781
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     terminator      6116..6159
                     /vntifkey="43"
                     /label=rrnB_T1_terminator
     terminator      6113..6159
                     /vntifkey="43"
                     /label=rrnB\T1\terminator
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     CDS             4987..6048
                     /codon_start=1
                     /vntifkey="4"
                     /label=che9c\61
     CDS             4050..4994
                     /codon_start=1
                     /vntifkey="4"
                     /label=che9c\60
     mRNA            768..2272
                     /vntifkey="54"
                     /label=pB264
BASE COUNT     1232 a      1882 c      1928 g      1205 t 
ORIGIN
        1 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 
       61 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg 
      121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
      181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
      241 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 
      301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
      361 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
      421 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
      481 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc 
      541 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
      601 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
      661 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
      721 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
      781 tcctgcgagg aacgtcgggc ggtcgagcgc ggcgtcgtcg cgtgtggagt tgcgcgagcg 
      841 ggtggtcgag gtcgagaagg tggtgcatcg gccggccacg acggccattg ctcgtttgaa 
      901 caggtctcac agaccatcag ggtgcaacgc acccgcacgg cagggctgtc ccaaggggga 
      961 cgggctggta gtggacgtca tcgcagctga tactcatcgc tcgattcctg ccagggcgtg 
     1021 atggacagtg ccgaccgagc atcccagctc attggcgata gctctcatgg acatgccagt 
     1081 accgcgtagc tcccgtatct tctcgcgccg ttgctgtgcc cgggacaggt aggcctctcg 
     1141 cggctctgat gtccaccgga tgatgctggc cgtcgatacg cctgtgcgtt cggcgagctc 
     1201 ccgcactgtt atcccgttgc gaggcagacg ctcagtcatg tgtggcctcc tcaattgcca 
     1261 gtcgcacctg atccagtttg gtgctgcgtg cacgccgggc ctggccgctg gcgacgccgc 
     1321 ctttgcggcc acgggcggat tggatggtcg agagtgtggc ttcgtagacg gcgataccgt 
     1381 ctttccagat gcgggatcgc gtggtgatcc atcggtgaat gctggcggcg atggcgcggg 
     1441 cttcagaggg tgggagttcc tcggtgtagc tggcgttgag ggcggtgact tcgcggtgga 
     1501 gggcgagttc gaggccggcg ctgtcatgag tgggcaggta gttgcgcatg aggccggggc 
     1561 gggtggccca ggtgcgagcg gcgtggaaga ttgcgcagtt gcggccgagt cctatggggg 
     1621 cgtcacggtg ggccttggtg tgctgccagc gcggggaggg catgtgtgtg ccgagttcgg 
     1681 cctcgagctc ggcgaggctg cgcggttcgg tgtggatcca gtgcgtgtcc cagcctgagt 
     1741 gtgtggggtt cttggtcatc aggcctgagt agccgatgtc cccttcgacg gcccggcgta 
     1801 gcccttcggt aacggcggcg gcgtacgcga ggggcttccg tcgggcgtac tcggtgcggg 
     1861 tgaacggctc gcgcagccac cagtgcaggt gcgcatgccc gttgctgctg ttttcgatca 
     1921 cggcgttcgg catagggtga ttgcctgccg ctgacagtgc gcgcagggcg gagtcggggt 
     1981 ggtcgacgtc gacgacgagc agattgctca gtgtctgcgg gttggcctcg atgtagcgcc 
     2041 gctcaagtgc ggcagggcgg cgcattcgat agaccccctc aaggaagtcg tttgttgcca 
     2101 aaggccagaa cggtagccac atttgctccc agtccccacc ttcccgcacc ccgtgtgtgt 
     2161 ccatgctggt gaccgtcgca tgctctcgaa cgaaacctgg gcatttccct cggtgtgttc 
     2221 aaagttaatc gtgaagccaa gtcagatgtt tcggtcttac ataaacgcga agaactccag 
     2281 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc 
     2341 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc 
     2401 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 
     2461 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 
     2521 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 
     2581 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 
     2641 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatccgc 
     2701 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 
     2761 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 
     2821 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 
     2881 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 
     2941 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 
     3001 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttggag ttcattcagg 
     3061 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 
     3121 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 
     3181 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct 
     3241 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa 
     3301 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat 
     3361 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg 
     3421 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg 
     3481 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt 
     3541 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctagcgtcta ccaggacttt 
     3601 tacctgtccg aagctccaag tcagatattt cgctgagggg ccaccccaca actgcacact 
     3661 cccccgctct cccgtcgagc cctggtggtg gaacaccagc gacagccgag cacccccaac 
     3721 cacctgtacc aaccactagt tgattagcta agcagaaggc catcctgacg gatggccttt 
     3781 ttgcgtttaa tactgtttaa actctagaaa tattggatcg tcggcaccgt cacggccgtg 
     3841 ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag 
     3901 ctctacgcca tcccggcgtt gatctgtgcg ttcgctgtgc gggctctaac acgtcctagt 
     3961 atggtaggat gagcaacatt tcgacgccga gagattcgcc gcccgaaatg agcacgatcc 
     4021 gcatgcttaa ttaagaagga gatatacata tgagtgtgcc cacacaggac ggaatgcacc 
     4081 ggttcgtcga cgaggacgtc taccacgctg accggggctc gctgtcggta tccggcgcga 
     4141 agctgctgtt gccgccgtcg tgtcccgcga aattccgctg ggagatggac aacacccgga 
     4201 agccgaaaaa ggtctgggac ttcggacatg tcgcgcacaa actggtgctc ggcaagggtg 
     4261 ccgagttcga gatcctcgac cccgaggtgc acgggctgaa ggcggacggt acgccgtcgg 
     4321 agaagccgac cgcgacgggc atgtggcgca aggccgaggc tgaggctcgc aaacagggca 
     4381 aggtgccgat tcacgtcgac ctgttcacga aggcgtacga catggccgaa aaggtgcgtc 
     4441 agcacccgac agccggcccg atcttcgcca atcctgacgg cgaggccgag gtcgcgctgt 
     4501 actacaccga ccccgagacc ggcgtgcggc tgcgtggccg gatcgactgg ctcactgacg 
     4561 atatcgatga ttacaagacg tcgatgaccg cgaacccggc cgagctgaaa accaagttct 
     4621 acaagctcgg ctatttcatg caggcggcct ggtacatcga tctactggtc gccctcgggc 
     4681 tcgccgagaa cccgcgattc cggttcatca cgcaggagaa agaaccgccc tacgtcgtga 
     4741 ctccgatcca gtacgacgac gaggcgatcg aagaggggcg gcgccgcaac cgccaggcga 
     4801 tccggctcta cgccgactgc atggaatcgg gcaagtggcc tgactacagc gacgacgtgg 
     4861 tcacgatcag cctgccctcg tgggggctgc cgcgaccgca gaccgtcggc gacgtcgtca 
     4921 ccgacagcta tatctacgac accgacccgc tcgaagaggc cgacccgatt gaaggggatt 
     4981 acatctatgg ctgaaaatgc tgtcaccaag caggattcgc ccaaggcacc cgagacgatc 
     5041 tcgcaagtgc tgcaggtgct cgtgccgcag ctggcgcgtg cagtgcccaa gggtatggac 
     5101 cccgaccgca tcgcgcggat cgtgcaaacc gagatccgca agtcgcgcaa cgcgaaagct 
     5161 gctggcatcg ctaagcagtc cctcgacgac tgcacgcaag agtcatttgc cggtgcgctg 
     5221 ctgacctcgg ccgcgctcgg tctcgagccc ggtgtcaacg gcgagtgcta cctcgtgccc 
     5281 taccgcgaca cccggcgcgg cgtggtcgag tgccagctga tcatcggcta ccagggcatc 
     5341 gtcaaactgt tctggcagca cccgcgcgcc tcccggatcg acgcgcagtg ggtcggcgcg 
     5401 aacgacgaat tccattacac catgggcctc aatccgacgc tgaaacacgt gaaggccaag 
     5461 ggtgatcggg gtaatccggt ctacttctac gcgatcgtcg aggtgaccgg cgctgagccg 
     5521 ctgtgggacg tgttcaccgc cgacgagatc agggaattgc gtcgcggcaa ggtcggatcc 
     5581 tcgggcgaca tcaaggaccc gcagcgctgg atggagcgga agaccgcgct caaacaggtg 
     5641 ctgaagctgg caccgaagac gacgcggctc gacgcggcga tccgcgccga cgatcgcccg 
     5701 ggcaccgacc tgtcacagtc gcaggcgctc gcgctgccgt cgaccgtcaa gccgacggcc 
     5761 gactacatcg acggcgagat cgccgagccg cacgaggtcg atacgccacc gaagtcgtcg 
     5821 cgcgcacaac gcgcgcagcg cgccaccgcg ccggcgcccg acgtgcagat ggccaatccc 
     5881 gatcagctga agcgcctcgg cgagatccag aaggccgaaa agtacaacga cgccgattgg 
     5941 ttcaagttcc tcgccgattc ggccggcgtc aaagccacgc gcgccgccga cctcacgttc 
     6001 gacgaggcga aggctgtcat cgacatgttc gacgggccca acgcatgagc gccccggcga 
     6061 attccgacgc ggtggttgat ctgcaactag cgtacgatcg actgccaggc atcaaataaa 
     6121 acgaaaggct cagtcgaaag actgggcctt tcgttttatg ccatcatggc cgcggtgatc 
     6181 agctagccac ctgacgtcgg ggggggggga aagccacgtt gtgtctcaaa atctctgatg 
     6241 ttacatt 
//
