LOCUS pDD143 9551 bp DNA circular 5-NOV-2018 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12540816| COMMENT VNTDBDATE|-12540816| COMMENT LSOWNER| COMMENT VNTNAME|pDD143| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pDD143" 0 9551 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 1 0 0 1 9531 1 9551 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (CObList) COMMENT (CObList COMMENT #0=(CFSignal (CObList) "GentR" 61 0 0 207 1331 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "207..1331") COMMENT #1=(CFSignal (CObList) "Sth1 sgRNA scaffold" 21 0 0 3552 3631 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3532..3611") COMMENT #2=(CFSignal (CObList) "Sth1 tracrRNA" 43 0 0 3602 3628 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3582..3608") COMMENT #3=(CFSignal (CObList) "TsynA" 43 0 0 3718 3755 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3698..3735") COMMENT #4=(CFSignal (CObList) "pMB1" 33 0 0 3844 4463 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3824..4443") COMMENT #5=(CFSignal (CObList) "rnBT1" 43 0 0 4492 4544 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4472..4524") COMMENT #6=(CFSignal (CObList) "TsynA" 43 0 0 4545 4582 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4525..4562") COMMENT #7=(CFSignal (CObList) "rrnB_T2_terminator" 43 0 0 3119 3146 0 COMMENT (CStringList) COMMENT (CStringList "/ApEinfo_fwdcolor=pink" "/ApEinfo_revcolor=pink" COMMENT "/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT "width 5 offset 0") 1 1 1 1 "3119..3146") COMMENT #8=(CFSignal (CObList) "pAL5000" 21 0 0 1660 2929 0 (CStringList) COMMENT (CStringList "/ApEinfo_fwdcolor=#ff6666" "/ApEinfo_revcolor=#ff6666" COMMENT "/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT "width 5 offset 0") 1 1 1 1 "1660..2929") COMMENT #9=(CFSignal (CObList) "araBAD promoter" 30 0 0 5660 5944 0 COMMENT (CStringList) (CStringList "/gene=\"araBAD\"") 1 1 1 1 "5640..5924") COMMENT #10=(CFSignal (CObList) "araC" 4 0 1 4755 5633 0 (CStringList) COMMENT (CStringList "/codon_start=1" "/gene=\"araC\"" COMMENT "/product=\"L-arabinose regulatory protein\"") 1 1 1 1 COMMENT "complement(4735..5613)") COMMENT #11=(CFSignal (CObList) "O2" 20 0 0 5663 5678 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5643..5658") COMMENT #12=(CFSignal (CObList) "O1" 20 0 0 5820 5841 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5800..5821") COMMENT #13=(CFSignal (CObList) "CAP" 20 0 0 5862 5875 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5842..5855") COMMENT #14=(CFSignal (CObList) "I1 & I2" 20 0 0 5872 5909 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5852..5889") COMMENT #15=(CFSignal (CObList) "-35" 48 0 0 5907 5911 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5887..5891") COMMENT #16=(CFSignal (CObList) "-10" 47 0 0 5931 5935 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5911..5915") COMMENT #17=(CFSignal (CObList) "Sth1 dCas9" 21 0 0 5981 9346 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5961..9326") COMMENT #18=(CFSignal (CObList) "rrnB T2 terminator" 43 0 0 9447 9474 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "9427..9454") COMMENT #19=(CFSignal (CObList) "TsynB" 43 0 0 9481 9518 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "9461..9498") COMMENT #20=(CFSignal (CObList) "pConstitutive" 21 0 0 3489 3529 0 (CStringList) COMMENT (CStringList "/ApEinfo_fwdcolor=#b3b3b3" COMMENT "/ApEinfo_revcolor=#800040" COMMENT "/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT "width 5 offset 0") 1 1 1 1 "3489..3529") COMMENT #21=(CFSignal (CObList) "-35 site" 21 0 0 3495 3498 0 (CStringList) COMMENT (CStringList "/ApEinfo_fwdcolor=cyan" "/ApEinfo_revcolor=green" COMMENT "/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT "width 5 offset 0") 1 1 1 1 "3495..3498") COMMENT #22=(CFSignal (CObList) "-10 site" 21 0 0 3518 3523 0 (CStringList) COMMENT (CStringList "/ApEinfo_fwdcolor=cyan" "/ApEinfo_revcolor=green" COMMENT "/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT "width 5 offset 0") 1 1 1 1 "3518..3523") COMMENT #23=(CFSignal (CObList) "TSS" 21 0 0 3531 3531 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3531..3531") COMMENT #24=(CFSignal (CObList) "sgRNA spacer" 21 0 0 3532 3551 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #25=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #26=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pDD143" 2) 5 "" 0 COMMENT 4) COMMENT #27=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pDD143'" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 11/05/18 07:51PM" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 11/05/18 07:51PM" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 9551 bp" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 6-NOV-2017" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: araBAD; araC" 1))) COMMENT "Standard Fields") COMMENT #39=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #40=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #42=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #43=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4755 (3 #10# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4755 End: 5633 (Complementary)" COMMENT 1) COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4735..5613)" 1) COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /codon_start=1" 1) COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"araC\"" 1) COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /product=\"L-arabinose regulatory protein\"" COMMENT 1))) "araC"))) "CDS (1 total)") COMMENT #51=(CFolderPar COMMENT (CGroupPar (CParagraph 20 (7 20 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #52=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5663 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5663 End: 5678" 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5643..5658" 1))) "O2") COMMENT #56=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5820 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5820 End: 5841" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5800..5821" 1))) "O1") COMMENT #60=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5862 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5862 End: 5875" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5842..5855" 1))) "CAP") COMMENT #64=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5872 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5872 End: 5909" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5852..5889" 1))) "I1 & I2"))) COMMENT "Misc. Binding Site (4 total)") COMMENT #68=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #69=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1660 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1660 End: 2929" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1660..2929" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_fwdcolor=#ff6666" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_revcolor=#ff6666" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " width 5 offset 0" 1))) "pAL5000") COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3489 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3489 End: 3529" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3489..3529" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_fwdcolor=#b3b3b3" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_revcolor=#800040" 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT 1) COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " width 5 offset 0" 1))) COMMENT "pConstitutive") COMMENT #87=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3495 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3495 End: 3498" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3495..3498" 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_fwdcolor=cyan" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_revcolor=green" 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " width 5 offset 0" 1))) "-35 site") COMMENT #96=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3518 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3518 End: 3523" 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3518..3523" 1) COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_fwdcolor=cyan" 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_revcolor=green" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT 1) COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " width 5 offset 0" 1))) "-10 site") COMMENT #105=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3531 (3 #23# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3531 End: 3531" 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3531..3531" 1))) "TSS") COMMENT #109=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3532 (3 #24# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3532 End: 3551" 1))) COMMENT "sgRNA spacer") COMMENT #111=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3552 (3 #1# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3552 End: 3631" 1) COMMENT #113=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Sth1 sgRNA scaffold" 1) COMMENT #114=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3532..3611" 1))) COMMENT "Sth1 sgRNA scaffold") COMMENT #116=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5981 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5981 End: 9346" 1) COMMENT #118=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Sth1 dCas9" 1) COMMENT #119=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5961..9326" 1))) "Sth1 dCas9"))) COMMENT "Misc. Feature (8 total)") COMMENT #121=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #122=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5660 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #123=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5660 End: 5944" 1) COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" COMMENT 1) COMMENT #125=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #126=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5640..5924" 1) COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"araBAD\"" 1))) COMMENT "araBAD promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #129=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #130=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3844 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3844 End: 4463" 1) COMMENT #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "oriE" 1) COMMENT #133=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #134=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3824..4443" 1))) "pMB1"))) COMMENT "Replication Origin (1 total)") COMMENT #135=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #136=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3119 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #137=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3119 End: 3146" 1) COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #139=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3119..3146" 1) COMMENT #140=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #141=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_fwdcolor=pink" 1) COMMENT #142=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_revcolor=pink" 1) COMMENT #143=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" COMMENT 1) COMMENT #144=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " width 5 offset 0" 1))) COMMENT "rrnB_T2_terminator") COMMENT #145=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3602 (3 #2# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3602 End: 3628" 1) COMMENT #147=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Sth1 tracrRNA term (hairpin -18.5 kcal/mol)" COMMENT 1) COMMENT #148=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #149=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3582..3608" 1))) "Sth1 tracrRNA") COMMENT #150=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3718 (3 #3# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #151=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3718 End: 3755" 1) COMMENT #152=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "TsynA (hairpin -15 kcal/mol)" 1) COMMENT #153=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #154=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3698..3735" 1))) "TsynA") COMMENT #155=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4492 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #156=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4492 End: 4544" 1) COMMENT #157=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "rnBT1 (hairpin -15.8 kcal/mol)" 1) COMMENT #158=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #159=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4472..4524" 1))) "rnBT1") COMMENT #160=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4545 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #161=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4545 End: 4582" 1) COMMENT #162=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "TsynA (hairpin -15 kcal/mol)" 1) COMMENT #163=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #164=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4525..4562" 1))) "TsynA") COMMENT #165=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 9447 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #166=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 9447 End: 9474" 1) COMMENT #167=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "rrnB T2 terminator (hairpin -14.7 kcal/mol)" COMMENT 1) COMMENT #168=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #169=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 9427..9454" 1))) COMMENT "rrnB T2 terminator") COMMENT #170=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 9481 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #171=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 9481 End: 9518" 1) COMMENT #172=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "TsynB (hairpin -14.2 kcal/mol)" 1) COMMENT #173=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #174=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 9461..9498" 1))) "TsynB"))) COMMENT "Terminator (7 total)") COMMENT #175=(CFolderPar COMMENT (CGroupPar (CParagraph 47 (7 47 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #176=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5931 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #177=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5931 End: 5935" 1) COMMENT #178=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #179=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5911..5915" 1))) "-10"))) COMMENT "-10 Signal (1 total)") COMMENT #180=(CFolderPar COMMENT (CGroupPar (CParagraph 48 (7 48 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #181=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5907 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #182=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5907 End: 5911" 1) COMMENT #183=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #184=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5887..5891" 1))) "-35"))) COMMENT "-35 Signal (1 total)") COMMENT #185=(CFolderPar COMMENT (CGroupPar (CParagraph 61 (7 61 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #186=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 207 (3 #0# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #187=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 207 End: 1331" 1) COMMENT #188=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #189=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 207..1331" 1))) "GentR"))) COMMENT "Exon (1 total)"))) "Feature Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #190=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #191=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #192=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #193=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #194=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #195=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #196=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #197=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #198=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #199=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #200=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #201=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #202=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #203=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #204=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #205=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #206=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #207=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #208=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #209=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #210=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #211=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #212=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #213=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #214=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #215=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #216=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #217=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #218=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #219=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #220=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #221=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #222=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #223=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #224=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #225=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #226=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #227=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #228=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #229=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -9531) 1 1 0 1 1 COMMENT (mapper: 18.2182 -14.4819 40.0378 40.0378 0.01 10 -1 9551 9551 1 0 0) COMMENT #230=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #231=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #232=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #196# 57849532 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27484 6.27284 1 0.0214037) 0.0656809) COMMENT #233=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #193# 413 100) COMMENT (LOGPEN 417 -515 4294966811) 436 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pDD143" "@N" 1 0 0.871165 0 -10 COMMENT 4.67059 1.47361 Nil) COMMENT #234=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #192# 8 100) COMMENT (LOGPEN 8 8 16) 8 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "9551 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.87229 0.949103 Nil)) (CObjectList)) COMMENT #235=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #236=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #237=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #194# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 2.57338 3.15071 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #238=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "araC" "@N" 1 COMMENT -5.36852 -15.681 0 -10 1.97314 1.07399 #237#)) COMMENT (CObjectList)) COMMENT #239=(CGroupWidget COMMENT (CWidget 20 (7 20 0) 1 2 0 0 Nil -182 100) COMMENT (CObjectList COMMENT #240=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 6 6723840) 8 1 2.55433 1.9 0.082322 COMMENT 1) COMMENT #241=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "O2" "@N" 1 COMMENT -8.188 -13.9386 0 -10 1.17389 1.07399 #240#) COMMENT #242=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #197# 51362800 COMMENT 100) (LOGPEN 0 6 6723840) 8 1 2.45121 1.9 COMMENT 0.082322 1) COMMENT #243=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "O1" "@N" 1 COMMENT -8.92852 -10.454 0 -10 1.17389 1.07399 #242#) COMMENT #244=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #197# 51471048 COMMENT 100) (LOGPEN 0 6 6723840) 8 1 2.42363 1.9 COMMENT 0.082322 1) COMMENT #245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "CAP" "@N" 1 COMMENT -9.74715 -8.71165 0 -10 1.89821 1.07399 #244#) COMMENT #246=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 6 6723840) 8 1 2.41706 1.9 0.082322 COMMENT 1) COMMENT #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "I1 & I2" "@N" 1 COMMENT -11.5385 -6.96932 0 -10 2.52262 1.07399 #246#)) COMMENT (CObjectList)) COMMENT #248=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #249=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #195# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.88831 3.94086 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #250=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Sth1 sgRNA scaffold" "@N" 1 13.8588 -12.1963 COMMENT 0 -10 8.34212 1.07399 #249#) COMMENT #251=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #195# 681 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 4.34939 5.18354 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pAL5000" "@N" 1 COMMENT 13.9794 0 0 -10 3.54665 1.07399 #251#) COMMENT #253=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #195# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.134646 COMMENT 2.34547 1 0.082322) 0.8 1.8 0) COMMENT #254=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1523678306 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Sth1 dCas9" COMMENT "@N" 1 -14.1685 3.48466 0 -10 4.62063 1.07399 COMMENT #253#) COMMENT #255=(CScratch COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #199# 51466968 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.98223 1.9 COMMENT 0.082322 1) COMMENT #256=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1537309970 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pConstitutive" COMMENT "@N" 1 14.6389 -3.48466 0 -10 5.41988 1.07399 COMMENT #255#) COMMENT #257=(CScratch COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #199# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 3.97829 1.9 COMMENT 0.082322 1) COMMENT #258=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1520008250 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "-35 site" "@N" COMMENT 1 12.6469 -5.22699 0 -10 2.92224 1.07399 COMMENT #257#) COMMENT #259=(CScratch COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #199# 908 100) COMMENT (LOGPEN 0 8 13395507) 10 1 3.96319 1.9 COMMENT 0.082322 1) COMMENT #260=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1533640426 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "-10 site" "@N" COMMENT 1 11.7476 -6.96932 0 -10 2.92224 1.07399 COMMENT #259#) COMMENT #261=(CScratch COMMENT (CWidget 0 (3 #23# 0) 1 2 0 0 #199# 50845544 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.95465 1.9 COMMENT 0.082322 1) COMMENT #262=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1510571466 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "TSS" "@N" 1 COMMENT 10.1679 -8.71165 0 -10 1.74835 1.07399 #261#) COMMENT #263=(CScratch COMMENT (CWidget 0 (3 #24# 0) 1 2 0 0 #199# 907 100) COMMENT (LOGPEN 0 8 13395507) 10 1 3.95399 1.9 COMMENT 0.082322 1) COMMENT #264=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1534426830 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "sgRNA spacer" COMMENT "@N" 1 12.5743 -10.454 0 -10 5.79452 1.07399 COMMENT #263#)) (CObjectList)) COMMENT #265=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #266=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #207# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 2.36911 2.5563 1 0.082322) 0.8 1.8 0) COMMENT #267=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "araBAD promoter" "@N" 1 -12.2496 -12.1963 0 COMMENT -10 7.0933 1.07399 #266#)) (CObjectList)) COMMENT #268=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -740 100) COMMENT (CObjectList COMMENT #269=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #194# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 3.34185 3.74907 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #270=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pMB1" "@N" 1 COMMENT 7.11194 -17.4233 0 -10 2.34778 1.07399 #269#)) COMMENT (CObjectList)) COMMENT #271=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -810 100) COMMENT (CObjectList COMMENT #272=(CScratch COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #199# 50846144 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.90802 1.9 COMMENT 0.082322 1) COMMENT #273=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Sth1 tracrRNA" COMMENT "@N" 1 12.437 -13.9386 0 -10 5.86945 1.07399 COMMENT #272#) COMMENT #274=(CScratch COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #199# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 3.83183 1.9 COMMENT 0.082322 1) COMMENT #275=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "TsynA" "@N" 1 COMMENT 10.0988 -15.681 0 -10 2.6475 1.07399 #274#) COMMENT #276=(CScratch COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #199# 51361904 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.32345 1.9 COMMENT 0.082322 1) COMMENT #277=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 10 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rnBT1" "@N" 1 COMMENT 4.68458 -19.1656 0 -10 2.52262 1.07399 #276#) COMMENT #278=(CScratch COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #199# 51363080 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.28864 1.9 COMMENT 0.082322 1) COMMENT #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "TsynA" "@N" 1 COMMENT 4.38746 -20.908 0 -10 2.6475 1.07399 #278#) COMMENT #280=(CScratch COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #199# 51363976 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 4.22525 1.9 COMMENT 0.082322 1) COMMENT #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "rrnB T2 terminator" "@N" 1 16.0637 -1.74233 COMMENT 0 -10 7.44297 1.07399 #280#) COMMENT #282=(CScratch COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #199# 908 100) COMMENT (LOGPEN 0 8 13395507) 10 1 0.068965 1.9 COMMENT 0.082322 1) COMMENT #283=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1527873186 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "rrnB T2 terminator" "@N" 1 -10.6725 10.454 0 COMMENT -10 7.44297 1.07399 #282#) COMMENT #284=(CScratch COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #199# 58261784 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 0.0466335 COMMENT 1.9 0.082322 1) COMMENT #285=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1530756742 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "TsynB" "@N" 1 COMMENT -2.82261 12.1963 0 -10 2.6475 1.07399 #284#)) COMMENT (CObjectList)) COMMENT #286=(CGroupWidget COMMENT (CWidget 47 (7 47 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #287=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #199# 50845944 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.37831 1.9 COMMENT 0.082322 1) COMMENT #288=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 1531805430 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "-10" "@N" 1 COMMENT -12.278 -3.48466 0 -10 1.29877 1.07399 #287#)) COMMENT (CObjectList)) COMMENT #289=(CGroupWidget COMMENT (CWidget 48 (7 48 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #290=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #199# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 2.39407 1.9 COMMENT 0.082322 1) COMMENT #291=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 529 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "-35" "@N" 1 COMMENT -11.706 -5.22699 0 -10 1.29877 1.07399 #290#)) COMMENT (CObjectList)) COMMENT #292=(CGroupWidget COMMENT (CWidget 61 (7 61 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList COMMENT #293=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #194# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 5.39897 6.13788 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #294=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "GentR" "@N" 1 COMMENT 8.00814 10.454 0 -10 2.59755 1.07399 #293#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #295=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList) (CObjectList)) COMMENT #296=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList) (CObjectList)) COMMENT #297=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1918975348 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1634165095 COMMENT (CStringList) 1952540515 1734833255 (CObList))) FEATURES Location/Qualifiers exon 207..1331 /vntifkey="61" /label=GentR misc_feature 3552..3631 /vntifkey="21" /label=Sth1\sgRNA\scaffold /note="Sth1 sgRNA scaffold" terminator 3602..3628 /vntifkey="43" /label=Sth1\tracrRNA /note="Sth1 tracrRNA term (hairpin -18.5 kcal/mol)" terminator 3718..3755 /vntifkey="43" /label=TsynA /note="TsynA (hairpin -15 kcal/mol)" rep_origin 3844..4463 /vntifkey="33" /label=pMB1 /note="oriE" terminator 4492..4544 /vntifkey="43" /label=rnBT1 /note="rnBT1 (hairpin -15.8 kcal/mol)" terminator 4545..4582 /vntifkey="43" /label=TsynA /note="TsynA (hairpin -15 kcal/mol)" terminator 3119..3146 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /vntifkey="43" /label=rrnB_T2_terminator misc_feature 1660..2929 /ApEinfo_fwdcolor=#ff6666 /ApEinfo_revcolor=#ff6666 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /vntifkey="21" /label=pAL5000 promoter 5660..5944 /gene="araBAD" /vntifkey="30" /label=araBAD\promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" CDS complement(4755..5633) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /vntifkey="4" /label=araC misc_binding 5663..5678 /vntifkey="20" /label=O2 misc_binding 5820..5841 /vntifkey="20" /label=O1 misc_binding 5862..5875 /vntifkey="20" /label=CAP misc_binding 5872..5909 /vntifkey="20" /label=I1\&\I2 -35_signal 5907..5911 /vntifkey="48" /label=-35 -10_signal 5931..5935 /vntifkey="47" /label=-10 misc_feature 5981..9346 /vntifkey="21" /label=Sth1\dCas9 /note="Sth1 dCas9" terminator 9447..9474 /vntifkey="43" /label=rrnB\T2\terminator /note="rrnB T2 terminator (hairpin -14.7 kcal/mol)" terminator 9481..9518 /vntifkey="43" /label=TsynB /note="TsynB (hairpin -14.2 kcal/mol)" misc_feature 3489..3529 /ApEinfo_fwdcolor=#b3b3b3 /ApEinfo_revcolor=#800040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /vntifkey="21" /label=pConstitutive misc_feature 3495..3498 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /vntifkey="21" /label=-35\site misc_feature 3518..3523 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /vntifkey="21" /label=-10\site misc_feature 3531..3531 /vntifkey="21" /label=TSS misc_feature 3532..3551 /vntifkey="21" /label=sgRNA\spacer BASE COUNT 2165 a 2852 c 2661 g 1853 t 20 others ORIGIN 1 gtaggacagg tgccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag 61 gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca 121 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc 181 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga 241 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca 301 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 361 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg 421 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 481 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca 541 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt 601 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc 661 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg 721 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct 781 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca 841 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag 901 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga 961 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct 1021 tcccggccgc ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt 1081 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac 1141 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg 1201 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc 1261 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc 1321 ggaaaagcgc tgcttccctg ctgttttgtg ggccgcaaag cgctccggct taactcaggc 1381 gttagtccca tcacccgctt tatgcgcgca gccacttatt tcagcgctcc gccctgttcc 1441 aaaggctttc tagccttttg ggcctccagc gcttgtgcag caagcgccac cagctatcct 1501 tcttgcaggg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg 1561 cggcagcgtg aagctagcgt ctaccaggac ttttacctgt ccgacccgtt gcaacggaac 1621 cccccacgga acccccgcga cacccgctcc ccaattgcgt tagaacagcg gtggattgtc 1681 ggcttcgttg tgggcctttt gagccgcttc ctgttctgcc gcacgctctt tcctcgcccg 1741 atagccgagt cgcttaacgg tgtccagatg cagcccgaaa tgtttggccg tttgcggcca 1801 agagtggccc tcgtcgtcgt gataggcgcg gatgcgttcg cggcgtgcag cctgctcggc 1861 gagccactcg ctgcgttcct gcgccacgag ccggacgacg tggcgttcgg atagtccggt 1921 gattcgagcg ccttcggcgg cggtcacgcg ccgctttttg cggacagtcg gctgccggtt 1981 gtagccgtcg ctgtagccgt cgctcatagc aatgcctcca tggctgacgc ggactttgcg 2041 cgccgcgcaa ctgtgctcgc cgccgtgcgc gctgctgcgc ccttccgcga gatggccgac 2101 tggcgcgcac tgagtgtggc ctcgtagacc acgatcccgt ccgcccaaat gcgcgacttg 2161 gttgtgatcc aacgccaaat gctgttggcg atggcgcgga cctcgctgtc cggtagcggt 2221 ccgggacaca cgtcgttgca cgggaactcg gcgtttcgcg cgtggcactc ggcatagatc 2281 gcgcggccga gtccgtccac gttccgggtc ggcaggtaga tccgcatgag ggcgggacga 2341 taggcccaca acctgacgga atcgaacagt gcgcaattcc gccctagcgg cgtcggagcc 2401 gctttgtacg tggtctgctg acgccagcgc ggcggtggca tgttcgcgcc gagctcggcc 2461 tcgatgtggc tgagtgtgta gagatctgag tggagccatt ccgtttccca ggcgatgtgg 2521 ccggggtttt tggtcatgag gcctgagtaa ctgcggtcgc cgtccacggc gcgccgaagg 2581 ccttcggcgc acgccgccat gtatgcgagc ggcttacgcc gcgcgtattc ggtgcgtgga 2641 acaggggcgt tgagtgccca cactgcgtgt gcgtggccgt tggcgcgatt gcccacgatc 2701 gcgttgggca gcggatggga cccccgggcg ctgagcgctc ggagcgctgc gtctggatgg 2761 tctacgtcca cgaccagcag gtttgccagc gctgttgggt tcgcctcgat gtaccggcgg 2821 cctagggccg acgcgcggct ttggcggtag atcccctcga gcagatcgtc gcttgccagc 2881 ggccagtacg gcagccagag ctgctcaaat tcgtcggcga cgtggctcac gcttggtagt 2941 agaccacgat taatcaccgg tgtatggtcc gacacgagct ccaagtcaga tatttcgctg 3001 aggggccacc ccacaactgc acactccccc gctctcccgt cgagccctgg tggtggaaca 3061 ccagcgacag ccgagcaccc ccaaccacct gtaccaacca ctagttgatt agctaagcag 3121 aaggccatcc tgacggatgg cctttttgcg tttaatactg tttaagctcc aagtcagata 3181 tttcgctgag gggccacccc acaactgcac actcccccgc tctcccgtcg agccctggtg 3241 gtggaacacc agcgacagcc gagcaccccc aaccacctgt accaaccact agttgattag 3301 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt taatactgtt taaactctag 3361 aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg 3421 atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt 3481 gcgttcgctg tgcgggctct aacacgtcct agtatggtag gatgagcaac annnnnnnnn 3541 nnnnnnnnnn ngtttttgta ctcgaaagaa gctacaaaga taaggcttca tgccgaaatc 3601 aacaccctgt cattttatgg cagggtgttt tttttttgtc gacttgggga ccctagaggt 3661 cccctttttt atttttttgg atgaagagca ttatcaatgg ctcttcacag gaagcttaaa 3721 aaaaaaagcc ccgcgattgc ggggcttttt ttttttaata cggttatcca cagaatcagg 3781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 4021 ctttctcccc tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 4081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 4141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4261 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 4321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcatggaa caataaacga 4501 aaggctcagt cgaaagactg ggcctttcgt ttatctgttg tttgaaaaaa aaaagccccg 4561 cgattgcggg gctttttttt ttcatatgtc tgaccaggga aaatagccct cagctccaag 4621 tcagatattt cgctgagggg ccaccccaca actgcacact cccccgctct cccgtcgagc 4681 cctggtggtg gaacaccagc gacagccgag cacccccaac cacctgtacc aaccactagt 4741 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg 4801 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt 4861 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa 4921 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac 4981 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg 5041 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc 5101 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat 5161 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta 5221 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac 5281 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga 5341 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc 5401 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt 5461 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt 5521 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga 5581 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc 5641 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac 5701 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt 5761 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac 5821 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt 5881 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 5941 ccatacagcg gataaagtag caaagagaag gaggttagga atgtcggacc tggtgctggg 6001 cctggccatc ggcatcggct cggtgggcgt gggcatcctg aacaaggtga ccggcgagat 6061 catccacaag aactcgcgca tcttcccggc cgcccaggcc gagaacaacc tggtgcgccg 6121 caccaaccgc cagggccgcc gcctggcccg ccgcaagaag caccgccgcg tgcgcctgaa 6181 ccgcctgttc gaggagtcgg gcctgatcac cgacttcacc aagatctcga tcaacctgaa 6241 cccgtaccag ctgcgcgtga agggcctgac cgacgagctg tcgaacgagg agctgttcat 6301 cgccctgaag aacatggtga agcaccgcgg catctcgtac ctggacgacg cctcggacga 6361 cggcaactcg tcggtgggcg actacgccca gatcgtgaag gagaactcga agcagctgga 6421 gaccaagacc ccgggccaga tccagctgga gcgctaccag acctacggcc agctgcgcgg 6481 cgacttcacc gtggagaagg acggcaagaa gcaccgcctg atcaacgtgt tcccgacctc 6541 ggcctaccgc tcggaggccc tgcgcatcct gcagacccag caggagttca acccgcagat 6601 caccgacgag ttcatcaacc gctacctgga gatcctgacc ggcaagcgca agtactacca 6661 cggcccgggc aacgagaagt cgcgcaccga ctacggccgc taccgcacct cgggcgagac 6721 cctggacaac atcttcggca tcctgatcgg caagtgcacc ttctacccgg acgagttccg 6781 cgccgccaag gcctcgtaca ccgcccagga gttcaacctg ctgaacgacc tgaacaacct 6841 gaccgtgccg accgagacca agaagctgtc gaaggagcag aagaaccaga tcatcaacta 6901 cgtgaagaac gagaaggcca tgggcccggc caagctgttc aagtacatcg ccaagctgct 6961 gtcgtgcgac gtggccgaca tcaagggcta ccgcatcgac aagtcgggca aggccgagat 7021 ccacaccttc gaggcctacc gcaagatgaa gaccctggag accctggaca tcgagcagat 7081 ggaccgcgag accctggaca agctggccta cgtgctgacc ctgaacaccg agcgcgaggg 7141 catccaggag gccctggagc acgagttcgc cgacggctcg ttctcgcaga agcaggtgga 7201 cgagctggtg cagttccgca aggccaactc gtcgatcttc ggcaagggct ggcacaactt 7261 ctcggtgaag ctgatgatgg agctgatccc ggagctgtac gagacctcgg aggagcagat 7321 gaccatcctg acccgcctgg gcaagcagaa gaccacctcg tcgtcgaaca agaccaagta 7381 catcgacgag aagctgctga ccgaggagat ctacaacccg gtggtggcca agtcggtgcg 7441 ccaggccatc aagatcgtga acgccgccat caaggagtac ggcgacttcg acaacatcgt 7501 gatcgagatg gcccgcgaga ccaacgagga cgacgagaag aaggccatcc agaagatcca 7561 gaaggccaac aaggacgaga aggacgccgc catgctgaag gccgccaacc agtacaacgg 7621 caaggccgag ctgccgcact cggtgttcca cggccacaag cagctggcca ccaagatccg 7681 cctgtggcac cagcagggcg agcgctgcct gtacaccggc aagaccatct cgatccacga 7741 cctgatcaac aactcgaacc agttcgaggt ggacgccatc ctgccgctgt cgatcacctt 7801 cgacgactcg ctggccaaca aggtgctggt gtacgccacc gccaaccagg agaagggcca 7861 gcgcaccccg taccaggccc tggactcgat ggacgacgcc tggtcgttcc gcgagctgaa 7921 ggccttcgtg cgcgagtcga agaccctgtc gaacaagaag aaggagtacc tgctgaccga 7981 ggaggacatc tcgaagttcg acgtgcgcaa gaagttcatc gagcgcaacc tggtggacac 8041 ccgctacgcc tcgcgcgtgg tgctgaacgc cctgcaggag cacttccgcg cccacaagat 8101 cgacaccaag gtgtcggtgg tgcgcggcca gttcacctcg cagctgcgcc gccactgggg 8161 catcgagaag acccgcgaca cctaccacca ccacgccgtg gacgccctga tcatcgccgc 8221 ctcgtcgcag ctgaacctgt ggaagaagca gaagaacacc ctggtgtcgt actcggagga 8281 ccagctgctg gacatcgaga ccggcgagct gatctcggac gacgagtaca aggagtcggt 8341 gttcaaggcc ccgtaccagc acttcgtgga caccctgaag tcgaaggagt tcgaggactc 8401 gatcctgttc tcgtaccagg tggactcgaa gttcaaccgc aagatctcgg acgccaccat 8461 ctacgccacc cgccaggcca aggtgggcaa ggacaaggcc gacgagacct acgtgctggg 8521 caagatcaag gacatctaca cccaggacgg ctacgacgcc ttcatgaaga tctacaagaa 8581 ggacaagtcg aagttcctga tgtaccgcca cgacccgcag accttcgaga aggtgatcga 8641 gccgatcctg gagaactacc cgaacaagca gatcaacgac aagggcaagg aggtgccgtg 8701 caacccgttc ctgaagtaca aggaggagca cggctacatc cgcaagtact cgaagaaggg 8761 caacggcccg gagatcaagt cgctgaagta ctacgactcg aagctgggca accacatcga 8821 catcaccccg aaggactcga acaacaaggt ggtgctgcag tcggtgtcgc cgtggcgcgc 8881 cgacgtgtac ttcaacaaga ccaccggcaa gtacgagatc ctgggcctga agtacgccga 8941 cctgcagttc gacaagggca ccggcaccta caagatctcg caggagaagt acaacgacat 9001 caagaagaag gagggcgtgg actcggactc ggagttcaag ttcaccctgt acaagaacga 9061 cctgctgctg gtgaaggaca ccgagaccaa ggagcagcag ctgttccgct tcctgtcgcg 9121 caccatgccg aagcagaagc actacgtgga gctgaagccg tacgacaagc agaagttcga 9181 gggcggcgag gccctgatca aggtgctggg caacgtggcc aactcgggcc agtgcaagaa 9241 gggcctgggc aagtcgaaca tctcgatcta caaggtgcgc accgacgtgc tgggcaacca 9301 gcacatcatc aagaacgagg gcgacaagcc gaagctggac ttctaaatac tctggccatt 9361 tttctttacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa 9421 cgcgtgcggc cgctgattag ctaagcagaa ggccatcctg acggatggcc tttttctaga 9481 aaaaaaaaaa gcgccgcaac tgcggcgctt tttttttttt aattaagtga tagattgcga 9541 tgcgtgtgct c //