LOCUS       pDD143                  9551 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12540816|
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FEATURES             Location/Qualifiers
     exon            207..1331
                     /vntifkey="61"
                     /label=GentR
     misc_feature    3552..3631
                     /vntifkey="21"
                     /label=Sth1\sgRNA\scaffold
                     /note="Sth1 sgRNA scaffold"
     terminator      3602..3628
                     /vntifkey="43"
                     /label=Sth1\tracrRNA
                     /note="Sth1 tracrRNA term (hairpin -18.5 kcal/mol)"
     terminator      3718..3755
                     /vntifkey="43"
                     /label=TsynA
                     /note="TsynA (hairpin -15 kcal/mol)"
     rep_origin      3844..4463
                     /vntifkey="33"
                     /label=pMB1
                     /note="oriE"
     terminator      4492..4544
                     /vntifkey="43"
                     /label=rnBT1
                     /note="rnBT1 (hairpin -15.8 kcal/mol)"
     terminator      4545..4582
                     /vntifkey="43"
                     /label=TsynA
                     /note="TsynA (hairpin -15 kcal/mol)"
     terminator      3119..3146
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     misc_feature    1660..2929
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pAL5000
     promoter        5660..5944
                     /gene="araBAD"
                     /vntifkey="30"
                     /label=araBAD\promoter
                     /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)"
     CDS             complement(4755..5633)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /vntifkey="4"
                     /label=araC
     misc_binding    5663..5678
                     /vntifkey="20"
                     /label=O2
     misc_binding    5820..5841
                     /vntifkey="20"
                     /label=O1
     misc_binding    5862..5875
                     /vntifkey="20"
                     /label=CAP
     misc_binding    5872..5909
                     /vntifkey="20"
                     /label=I1\&\I2
     -35_signal      5907..5911
                     /vntifkey="48"
                     /label=-35
     -10_signal      5931..5935
                     /vntifkey="47"
                     /label=-10
     misc_feature    5981..9346
                     /vntifkey="21"
                     /label=Sth1\dCas9
                     /note="Sth1 dCas9"
     terminator      9447..9474
                     /vntifkey="43"
                     /label=rrnB\T2\terminator
                     /note="rrnB T2 terminator (hairpin -14.7 kcal/mol)"
     terminator      9481..9518
                     /vntifkey="43"
                     /label=TsynB
                     /note="TsynB (hairpin -14.2 kcal/mol)"
     misc_feature    3489..3529
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pConstitutive
     misc_feature    3495..3498
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-35\site
     misc_feature    3518..3523
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-10\site
     misc_feature    3531..3531
                     /vntifkey="21"
                     /label=TSS
     misc_feature    3532..3551
                     /vntifkey="21"
                     /label=sgRNA\spacer
BASE COUNT     2165 a      2852 c      2661 g      1853 t        20 others
ORIGIN
        1 gtaggacagg tgccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag 
       61 gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca 
      121 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc 
      181 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga 
      241 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca 
      301 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 
      361 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg 
      421 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 
      481 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca 
      541 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt 
      601 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc 
      661 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg 
      721 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct 
      781 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca 
      841 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag 
      901 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga 
      961 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct 
     1021 tcccggccgc ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt 
     1081 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac 
     1141 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg 
     1201 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc 
     1261 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc 
     1321 ggaaaagcgc tgcttccctg ctgttttgtg ggccgcaaag cgctccggct taactcaggc 
     1381 gttagtccca tcacccgctt tatgcgcgca gccacttatt tcagcgctcc gccctgttcc 
     1441 aaaggctttc tagccttttg ggcctccagc gcttgtgcag caagcgccac cagctatcct 
     1501 tcttgcaggg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg 
     1561 cggcagcgtg aagctagcgt ctaccaggac ttttacctgt ccgacccgtt gcaacggaac 
     1621 cccccacgga acccccgcga cacccgctcc ccaattgcgt tagaacagcg gtggattgtc 
     1681 ggcttcgttg tgggcctttt gagccgcttc ctgttctgcc gcacgctctt tcctcgcccg 
     1741 atagccgagt cgcttaacgg tgtccagatg cagcccgaaa tgtttggccg tttgcggcca 
     1801 agagtggccc tcgtcgtcgt gataggcgcg gatgcgttcg cggcgtgcag cctgctcggc 
     1861 gagccactcg ctgcgttcct gcgccacgag ccggacgacg tggcgttcgg atagtccggt 
     1921 gattcgagcg ccttcggcgg cggtcacgcg ccgctttttg cggacagtcg gctgccggtt 
     1981 gtagccgtcg ctgtagccgt cgctcatagc aatgcctcca tggctgacgc ggactttgcg 
     2041 cgccgcgcaa ctgtgctcgc cgccgtgcgc gctgctgcgc ccttccgcga gatggccgac 
     2101 tggcgcgcac tgagtgtggc ctcgtagacc acgatcccgt ccgcccaaat gcgcgacttg 
     2161 gttgtgatcc aacgccaaat gctgttggcg atggcgcgga cctcgctgtc cggtagcggt 
     2221 ccgggacaca cgtcgttgca cgggaactcg gcgtttcgcg cgtggcactc ggcatagatc 
     2281 gcgcggccga gtccgtccac gttccgggtc ggcaggtaga tccgcatgag ggcgggacga 
     2341 taggcccaca acctgacgga atcgaacagt gcgcaattcc gccctagcgg cgtcggagcc 
     2401 gctttgtacg tggtctgctg acgccagcgc ggcggtggca tgttcgcgcc gagctcggcc 
     2461 tcgatgtggc tgagtgtgta gagatctgag tggagccatt ccgtttccca ggcgatgtgg 
     2521 ccggggtttt tggtcatgag gcctgagtaa ctgcggtcgc cgtccacggc gcgccgaagg 
     2581 ccttcggcgc acgccgccat gtatgcgagc ggcttacgcc gcgcgtattc ggtgcgtgga 
     2641 acaggggcgt tgagtgccca cactgcgtgt gcgtggccgt tggcgcgatt gcccacgatc 
     2701 gcgttgggca gcggatggga cccccgggcg ctgagcgctc ggagcgctgc gtctggatgg 
     2761 tctacgtcca cgaccagcag gtttgccagc gctgttgggt tcgcctcgat gtaccggcgg 
     2821 cctagggccg acgcgcggct ttggcggtag atcccctcga gcagatcgtc gcttgccagc 
     2881 ggccagtacg gcagccagag ctgctcaaat tcgtcggcga cgtggctcac gcttggtagt 
     2941 agaccacgat taatcaccgg tgtatggtcc gacacgagct ccaagtcaga tatttcgctg 
     3001 aggggccacc ccacaactgc acactccccc gctctcccgt cgagccctgg tggtggaaca 
     3061 ccagcgacag ccgagcaccc ccaaccacct gtaccaacca ctagttgatt agctaagcag 
     3121 aaggccatcc tgacggatgg cctttttgcg tttaatactg tttaagctcc aagtcagata 
     3181 tttcgctgag gggccacccc acaactgcac actcccccgc tctcccgtcg agccctggtg 
     3241 gtggaacacc agcgacagcc gagcaccccc aaccacctgt accaaccact agttgattag 
     3301 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt taatactgtt taaactctag 
     3361 aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg 
     3421 atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt 
     3481 gcgttcgctg tgcgggctct aacacgtcct agtatggtag gatgagcaac annnnnnnnn 
     3541 nnnnnnnnnn ngtttttgta ctcgaaagaa gctacaaaga taaggcttca tgccgaaatc 
     3601 aacaccctgt cattttatgg cagggtgttt tttttttgtc gacttgggga ccctagaggt 
     3661 cccctttttt atttttttgg atgaagagca ttatcaatgg ctcttcacag gaagcttaaa 
     3721 aaaaaaagcc ccgcgattgc ggggcttttt ttttttaata cggttatcca cagaatcagg 
     3781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     3841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     3901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     3961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     4021 ctttctcccc tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     4081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     4141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     4201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     4261 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     4321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     4381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     4441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcatggaa caataaacga 
     4501 aaggctcagt cgaaagactg ggcctttcgt ttatctgttg tttgaaaaaa aaaagccccg 
     4561 cgattgcggg gctttttttt ttcatatgtc tgaccaggga aaatagccct cagctccaag 
     4621 tcagatattt cgctgagggg ccaccccaca actgcacact cccccgctct cccgtcgagc 
     4681 cctggtggtg gaacaccagc gacagccgag cacccccaac cacctgtacc aaccactagt 
     4741 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg 
     4801 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt 
     4861 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa 
     4921 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac 
     4981 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg 
     5041 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc 
     5101 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat 
     5161 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta 
     5221 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac 
     5281 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga 
     5341 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc 
     5401 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt 
     5461 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt 
     5521 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga 
     5581 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc 
     5641 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac 
     5701 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt 
     5761 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac 
     5821 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt 
     5881 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 
     5941 ccatacagcg gataaagtag caaagagaag gaggttagga atgtcggacc tggtgctggg 
     6001 cctggccatc ggcatcggct cggtgggcgt gggcatcctg aacaaggtga ccggcgagat 
     6061 catccacaag aactcgcgca tcttcccggc cgcccaggcc gagaacaacc tggtgcgccg 
     6121 caccaaccgc cagggccgcc gcctggcccg ccgcaagaag caccgccgcg tgcgcctgaa 
     6181 ccgcctgttc gaggagtcgg gcctgatcac cgacttcacc aagatctcga tcaacctgaa 
     6241 cccgtaccag ctgcgcgtga agggcctgac cgacgagctg tcgaacgagg agctgttcat 
     6301 cgccctgaag aacatggtga agcaccgcgg catctcgtac ctggacgacg cctcggacga 
     6361 cggcaactcg tcggtgggcg actacgccca gatcgtgaag gagaactcga agcagctgga 
     6421 gaccaagacc ccgggccaga tccagctgga gcgctaccag acctacggcc agctgcgcgg 
     6481 cgacttcacc gtggagaagg acggcaagaa gcaccgcctg atcaacgtgt tcccgacctc 
     6541 ggcctaccgc tcggaggccc tgcgcatcct gcagacccag caggagttca acccgcagat 
     6601 caccgacgag ttcatcaacc gctacctgga gatcctgacc ggcaagcgca agtactacca 
     6661 cggcccgggc aacgagaagt cgcgcaccga ctacggccgc taccgcacct cgggcgagac 
     6721 cctggacaac atcttcggca tcctgatcgg caagtgcacc ttctacccgg acgagttccg 
     6781 cgccgccaag gcctcgtaca ccgcccagga gttcaacctg ctgaacgacc tgaacaacct 
     6841 gaccgtgccg accgagacca agaagctgtc gaaggagcag aagaaccaga tcatcaacta 
     6901 cgtgaagaac gagaaggcca tgggcccggc caagctgttc aagtacatcg ccaagctgct 
     6961 gtcgtgcgac gtggccgaca tcaagggcta ccgcatcgac aagtcgggca aggccgagat 
     7021 ccacaccttc gaggcctacc gcaagatgaa gaccctggag accctggaca tcgagcagat 
     7081 ggaccgcgag accctggaca agctggccta cgtgctgacc ctgaacaccg agcgcgaggg 
     7141 catccaggag gccctggagc acgagttcgc cgacggctcg ttctcgcaga agcaggtgga 
     7201 cgagctggtg cagttccgca aggccaactc gtcgatcttc ggcaagggct ggcacaactt 
     7261 ctcggtgaag ctgatgatgg agctgatccc ggagctgtac gagacctcgg aggagcagat 
     7321 gaccatcctg acccgcctgg gcaagcagaa gaccacctcg tcgtcgaaca agaccaagta 
     7381 catcgacgag aagctgctga ccgaggagat ctacaacccg gtggtggcca agtcggtgcg 
     7441 ccaggccatc aagatcgtga acgccgccat caaggagtac ggcgacttcg acaacatcgt 
     7501 gatcgagatg gcccgcgaga ccaacgagga cgacgagaag aaggccatcc agaagatcca 
     7561 gaaggccaac aaggacgaga aggacgccgc catgctgaag gccgccaacc agtacaacgg 
     7621 caaggccgag ctgccgcact cggtgttcca cggccacaag cagctggcca ccaagatccg 
     7681 cctgtggcac cagcagggcg agcgctgcct gtacaccggc aagaccatct cgatccacga 
     7741 cctgatcaac aactcgaacc agttcgaggt ggacgccatc ctgccgctgt cgatcacctt 
     7801 cgacgactcg ctggccaaca aggtgctggt gtacgccacc gccaaccagg agaagggcca 
     7861 gcgcaccccg taccaggccc tggactcgat ggacgacgcc tggtcgttcc gcgagctgaa 
     7921 ggccttcgtg cgcgagtcga agaccctgtc gaacaagaag aaggagtacc tgctgaccga 
     7981 ggaggacatc tcgaagttcg acgtgcgcaa gaagttcatc gagcgcaacc tggtggacac 
     8041 ccgctacgcc tcgcgcgtgg tgctgaacgc cctgcaggag cacttccgcg cccacaagat 
     8101 cgacaccaag gtgtcggtgg tgcgcggcca gttcacctcg cagctgcgcc gccactgggg 
     8161 catcgagaag acccgcgaca cctaccacca ccacgccgtg gacgccctga tcatcgccgc 
     8221 ctcgtcgcag ctgaacctgt ggaagaagca gaagaacacc ctggtgtcgt actcggagga 
     8281 ccagctgctg gacatcgaga ccggcgagct gatctcggac gacgagtaca aggagtcggt 
     8341 gttcaaggcc ccgtaccagc acttcgtgga caccctgaag tcgaaggagt tcgaggactc 
     8401 gatcctgttc tcgtaccagg tggactcgaa gttcaaccgc aagatctcgg acgccaccat 
     8461 ctacgccacc cgccaggcca aggtgggcaa ggacaaggcc gacgagacct acgtgctggg 
     8521 caagatcaag gacatctaca cccaggacgg ctacgacgcc ttcatgaaga tctacaagaa 
     8581 ggacaagtcg aagttcctga tgtaccgcca cgacccgcag accttcgaga aggtgatcga 
     8641 gccgatcctg gagaactacc cgaacaagca gatcaacgac aagggcaagg aggtgccgtg 
     8701 caacccgttc ctgaagtaca aggaggagca cggctacatc cgcaagtact cgaagaaggg 
     8761 caacggcccg gagatcaagt cgctgaagta ctacgactcg aagctgggca accacatcga 
     8821 catcaccccg aaggactcga acaacaaggt ggtgctgcag tcggtgtcgc cgtggcgcgc 
     8881 cgacgtgtac ttcaacaaga ccaccggcaa gtacgagatc ctgggcctga agtacgccga 
     8941 cctgcagttc gacaagggca ccggcaccta caagatctcg caggagaagt acaacgacat 
     9001 caagaagaag gagggcgtgg actcggactc ggagttcaag ttcaccctgt acaagaacga 
     9061 cctgctgctg gtgaaggaca ccgagaccaa ggagcagcag ctgttccgct tcctgtcgcg 
     9121 caccatgccg aagcagaagc actacgtgga gctgaagccg tacgacaagc agaagttcga 
     9181 gggcggcgag gccctgatca aggtgctggg caacgtggcc aactcgggcc agtgcaagaa 
     9241 gggcctgggc aagtcgaaca tctcgatcta caaggtgcgc accgacgtgc tgggcaacca 
     9301 gcacatcatc aagaacgagg gcgacaagcc gaagctggac ttctaaatac tctggccatt 
     9361 tttctttacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa 
     9421 cgcgtgcggc cgctgattag ctaagcagaa ggccatcctg acggatggcc tttttctaga 
     9481 aaaaaaaaaa gcgccgcaac tgcggcgctt tttttttttt aattaagtga tagattgcga 
     9541 tgcgtgtgct c 
//
