LOCUS       pDD56                   6013 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12540801|
COMMENT     VNTDBDATE|-12540801|
COMMENT     LSOWNER|
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COMMENT                                           "promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" 
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FEATURES             Location/Qualifiers
     misc_feature    978..1976
                     /ApEinfo_fwdcolor=#ff6fcf
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=KanR\cassette
     misc_feature    2212..3481
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pAL5000
     misc_feature    91..710
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pMB1\ori
     -10_signal      4848..4852
                     /vntifkey="47"
                     /label=-10
     -35_signal      4824..4828
                     /vntifkey="48"
                     /label=-35
     misc_binding    4789..4826
                     /vntifkey="20"
                     /label=I1\&\I2
     misc_binding    4779..4792
                     /vntifkey="20"
                     /label=CAP
     misc_binding    4737..4758
                     /vntifkey="20"
                     /label=O1
     misc_binding    4580..4595
                     /vntifkey="20"
                     /label=O2
     misc_feature    4898..5623
                     /vntifkey="21"
                     /label=EYFP
     CDS             complement(3672..4550)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /vntifkey="4"
                     /label=araC
     promoter        4577..4861
                     /gene="araBAD"
                     /vntifkey="30"
                     /label=araBAD\promoter
                     /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)"
     terminator      5659..5730
                     /vntifkey="43"
                     /label=rrnB\T1\terminator
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      5747..5818
                     /vntifkey="43"
                     /label=rrnB\T1\terminator
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      5840..5897
                     /vntifkey="43"
                     /label=his\operon\terminator
                     /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined."
BASE COUNT     1306 a      1794 c      1653 g      1260 t 
ORIGIN
        1 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
       61 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
      121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
      181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
      241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
      301 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
      361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
      421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
      481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
      541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
      601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
      661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
      721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
      781 aaggatcttc acctagatcc ttttggggtg ggcgaagaac tccagcatga gatccccgcg 
      841 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 
      901 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 
      961 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 
     1021 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 
     1081 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc 
     1141 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 
     1201 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg 
     1261 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 
     1321 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 
     1381 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 
     1441 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 
     1501 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 
     1561 gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg 
     1621 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 
     1681 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 
     1741 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 
     1801 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact 
     1861 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct 
     1921 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt 
     1981 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt 
     2041 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc 
     2101 cctgagtgct tgcggcagcg tgaagctagc gtctaccagg acttttacct gtccgacccg 
     2161 ttgcaacgga accccccacg gaacccccgc gacacccgct ccccaattgc gttagaacag 
     2221 cggtggattg tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg ccgcacgctc 
     2281 tttcctcgcc cgatagccga gtcgcttaac ggtgtccaga tgcagcccga aatgtttggc 
     2341 cgtttgcggc caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt cgcggcgtgc 
     2401 agcctgctcg gcgagccact cgctgcgttc ctgcgccacg agccggacga cgtggcgttc 
     2461 ggatagtccg gtgattcgag cgccttcggc ggcggtcacg cgccgctttt tgcggacagt 
     2521 cggctgccgg ttgtagccgt cgctgtagcc gtcgctcata gcaatgcctc catggctgac 
     2581 gcggactttg cgcgccgcgc aactgtgctc gccgccgtgc gcgctgctgc gcccttccgc 
     2641 gagatggccg actggcgcgc actgagtgtg gcctcgtaga ccacgatccc gtccgcccaa 
     2701 atgcgcgact tggttgtgat ccaacgccaa atgctgttgg cgatggcgcg gacctcgctg 
     2761 tccggtagcg gtccgggaca cacgtcgttg cacgggaact cggcgtttcg cgcgtggcac 
     2821 tcggcataga tcgcgcggcc gagtccgtcc acgttccggg tcggcaggta gatccgcatg 
     2881 agggcgggac gataggccca caacctgacg gaatcgaaca gtgcgcaatt ccgccctagc 
     2941 ggcgtcggag ccgctttgta cgtggtctgc tgacgccagc gcggcggtgg catgttcgcg 
     3001 ccgagctcgg cctcgatgtg gctgagtgtg tagagatctg agtggagcca ttccgtttcc 
     3061 caggcgatgt ggccggggtt tttggtcatg aggcctgagt aactgcggtc gccgtccacg 
     3121 gcgcgccgaa ggccttcggc gcacgccgcc atgtatgcga gcggcttacg ccgcgcgtat 
     3181 tcggtgcgtg gaacaggggc gttgagtgcc cacactgcgt gtgcgtggcc gttggcgcga 
     3241 ttgcccacga tcgcgttggg cagcggatgg gacccccggg cgctgagcgc tcggagcgct 
     3301 gcgtctggat ggtctacgtc cacgaccagc aggtttgcca gcgctgttgg gttcgcctcg 
     3361 atgtaccggc ggcctagggc cgacgcgcgg ctttggcggt agatcccctc gagcagatcg 
     3421 tcgcttgcca gcggccagta cggcagccag agctgctcaa attcgtcggc gacgtggctc 
     3481 acgcttggta gtagaccacg attaatcacc ggtgtatggt ccgacacgag ctccaagtca 
     3541 gatatttcgc tgaggggcca ccccacaact gcacactccc ccgctctccc gtcgagccct 
     3601 ggtggtggaa caccagcgac agccgagcac ccccaaccac ctgtaccaac cactagttga 
     3661 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca 
     3721 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga 
     3781 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc 
     3841 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc 
     3901 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg 
     3961 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga 
     4021 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc 
     4081 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg 
     4141 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc 
     4201 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag 
     4261 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct 
     4321 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc 
     4381 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg 
     4441 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg 
     4501 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata 
     4561 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc 
     4621 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg 
     4681 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc 
     4741 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta 
     4801 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca 
     4861 tacagcggat aaagtagcaa agagaaggag gttaggaatg gtgagcaagg gcgaggagct 
     4921 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 
     4981 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 
     5041 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg 
     5101 cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc 
     5161 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 
     5221 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 
     5281 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 
     5341 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 
     5401 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 
     5461 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct 
     5521 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 
     5581 cgggatcact ctcggcatgg acgagctgta caaggctgcg aatggtctgg tgtcaaaaat 
     5641 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 
     5701 tttatctgtt gtttgtcggt gaacgctctc tactagagcc aggcatcaaa taaaacgaaa 
     5761 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 
     5821 ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt 
     5881 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc 
     5941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     6001 ccacagaatc agg 
//
