LOCUS       pDD108                  4330 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12540773|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     promoter        complement(1535..1569)
                     /vntifkey="30"
                     /label=BBa_J23104
     misc_feature    complement(737..1460)
                     /vntifkey="21"
                     /label=EYFP
     terminator      complement(586..715)
                     /vntifkey="43"
                     /label=Terminator
     CDS             complement(1910..2554)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=HygR
     terminator      1835..1866
                     /ApEinfo_label=T4_32_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=T4_32_terminator(1)
     rep_origin      3539..4256
                     /vntifkey="33"
                     /label=p15A
     misc_feature    1..495
                     /vntifkey="21"
                     /label=Upstream\Homologous\Arm
     misc_feature    2924..3454
                     /vntifkey="21"
                     /label=Downstream\Homologous\Arm
BASE COUNT      859 a      1311 c      1286 g       874 t 
ORIGIN
        1 gcagatacgt gtgctgatcc aacgcctgcg cactcatcac gaaccccttc cattcgaaag 
       61 aatcgaaaca cttttcgaat ctttcgaata gctggttcaa tcaccgccgc ttacatggcg 
      121 agatcgacca cgtcccacct gccgagtacg agaccgccta ttggtcgaat cacacggcgg 
      181 cactacggtg agacaccggt cctcgctgag gccggaacca gataaccgag cctccgccga 
      241 actcggggca ggtgtcaccg ccccggtcct cgagagcaag ctcgacggtg tcagcgaaat 
      301 gcgtgaacac ctcagccgcg aactggccca gcagcccacc cgccgcatcc ctatccggct 
      361 cagcgacgaa tgcacccgcg ccaacaccgc cgcccgcaca gccagccaga acctcgaaac 
      421 ctgaatccgc gaatgtgtgc ggtcgccgcc gcagtgctcg ggcattccgg ccgaaccgtt 
      481 gtgcctgtct caatccctga ttctgtgcga tagtgagtta tgcaaccaaa tgacgtggct 
      541 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag ctcgatataa acgcagaaag 
      601 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga 
      661 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggttatca 
      721 tgagaccggt ctcaattcgc agccttgtac agctcgtcca tgccgagagt gatcccggcg 
      781 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg 
      841 gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg 
      901 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc 
      961 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg 
     1021 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc 
     1081 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg 
     1141 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg 
     1201 gacttgaaga agtcgtgcag cttcatgtgg tcggggtagc gggcgaagca ttgcaggccg 
     1261 tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg 
     1321 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac 
     1381 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc 
     1441 tcctcgccct tgctcaccat ctagtatttc tcctctttct ctagtatgtg tgaaattgtt 
     1501 atccgcttct aagcaaacct taacagcaga gaaggctagc acaataccta ggactgagct 
     1561 agctgtcaac tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa 
     1621 tgtcatgata ataatggttt cttagacgtc gatatctggc gaaaatggtt aatcaagggc 
     1681 actctcgtat tgaccaccct ggaggagatg atcgaggatg gtgcagagcc agccttctta 
     1741 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct 
     1801 taatgaccat gggctagcgg ctttagctaa ttaattgggg accctagagg tccccttttt 
     1861 tattttgcgg ccgccggggg ccgggggcgg cgccgggcgg cccggggcgt caggcgccgg 
     1921 gggcggtgtc cggcggcccc cagaggaact gcgccagttc ctccggatcg gtgaagccgg 
     1981 agagatccag cggggtctcc tcgaacacct cgaagtcgtg caggaaggtg aaggcgagca 
     2041 gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc gtcgagcagc gcggccagga 
     2101 tctcgcggtc gccccggaag gcgttgagat gcagttgcac caggctgtag cgggagtctc 
     2161 ccgcatagac gtcggtgaag tcgacgatcc cggtgacctc ggtcgcggcc aggtccacga 
     2221 agatgttggt cccgtgcagg tcgccgtgga cgaaccgggg ttcgcggccg gccagcagcg 
     2281 tgtccacgtc cggcagccag tcctccaggc ggtccagcag ccggggcgag aggtagcccc 
     2341 acccgcggtg gtcctcgacg gtcgccgcgc ggcgttcccg cagcagttcc gggaagacct 
     2401 cggaatgggg ggtgagcacg gtgttcccgg tcagcggcac cctgtgcagc cggccgagca 
     2461 cccggccgag ttcgcgggcc agggcgagca gcgcgttccg gtcggtcgtg ccgtccatcg 
     2521 cggaccgcca ggtggtgccg gtcatccggc tcatcaccag gtagggccac ggccaggctc 
     2581 cggtgccggg ccgcagctcg ccgcggccga ggaggcgggg caccggcacc ggggcgtccg 
     2641 ccaggaccgc gtacgcctcc gactccgacg cgaggctctc cggaccgcac cagtgctcgc 
     2701 cgaacagctt gatcaccggg tcgggctcgc cgaccagtac ggggttggtg ctctcgccgg 
     2761 gcacccgcag caccggcggc accggcagcc cgagctcctc cagggctcgg cgggccagcg 
     2821 gctcccagaa ttcctggtcg ttccgcaggc tcgcgtagga atcatccgaa tcaatacggt 
     2881 cgagaagtaa cagggattct tgtgtcacag cggacctcta ttcggtactg cgcgagaggg 
     2941 taaacggctg ccacccgtcc ctgtcgccgt acgcgggcat cagcgacgct ccccaaacgt 
     3001 cgactgccac tgccactgcc actgccactg ccactgccac tgagcaacga actcgacgag 
     3061 ctcgggcagc gccagcgctg caagcccagc agaactcatg cgcggggcgg tcgaggaata 
     3121 catcttcgcc tgaaacaccc cccgacgctc gcggagcaac ctgtcgaaac accacctcgg 
     3181 attccgtcgc acaatcgaaa gggaggagaa ggcggaagga cctgctggag gtgaccatgc 
     3241 gtcgggagga actggcaccg gtgcccacgc cggtccggtg gtggcggctc ggcccatgga 
     3301 gccgcaacca cctgatgcgc cccagcgacc gctacgaggc caccatcatc ctcatcatcc 
     3361 tgctcaccgc ggccgtggtc ctgctgctga tccccgttgc ggccgcgttc ggcacctcga 
     3421 cgcagacccg gttggaacag caaacccaca cactgagata ggtgctgttg tttgtcggtg 
     3481 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc 
     3541 tagggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc 
     3601 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta 
     3661 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa 
     3721 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata 
     3781 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac 
     3841 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg 
     3901 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct 
     3961 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac 
     4021 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa 
     4081 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc 
     4141 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt 
     4201 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag 
     4261 ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc 
     4321 cccatacgct 
//
