LOCUS       pDD132                  4238 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12540769|
COMMENT     LSOWNER|
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.90272 3.9744 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #156=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EYFP" "@N" 1 
COMMENT                                   5.55089 -12.1963 0 -10 2.29783 1.07399 #155#) 
COMMENT                             #157=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #110# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #158=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Upstream homologous arm" "@N" 1 18.1784 0 0 
COMMENT                                   -10 10.8897 1.07399 #157#) 
COMMENT                             #159=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #110# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   10.454 0 -10 12.0136 1.07399 #159#)) 
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FEATURES             Location/Qualifiers
     rep_origin      85..802
                     /vntifkey="33"
                     /label=p15A
     terminator      2652..2683
                     /ApEinfo_label=T4_32_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=T4_32_terminator(1)
     CDS             complement(2727..3371)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=HygR
     terminator      complement(1403..1532)
                     /vntifkey="43"
                     /label=Terminator
     misc_feature    complement(1554..2277)
                     /vntifkey="21"
                     /label=EYFP
     promoter        complement(2352..2386)
                     /vntifkey="30"
                     /label=BBa_J23104
     misc_feature    877..1312
                     /vntifkey="21"
                     /label=Upstream\homologous\arm
     misc_feature    3741..4238
                     /vntifkey="21"
                     /label=Downstream\homologous\arm
BASE COUNT      812 a      1231 c      1284 g       911 t 
ORIGIN
        1 gagataggtg ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 
       61 cgggtgggcc tttctgcgtt tatacctagg gatatattcc gcttcctcgc tcactgactc 
      121 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt 
      181 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt 
      241 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg 
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc 
      361 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct 
      421 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca 
      481 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
      541 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt 
      601 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg 
      661 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac 
      721 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat 
      781 ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct 
      841 cttcaaatgt agcacctgaa gtcagcccca tacgctcgcc ttgtcgtgga tcttctttcc 
      901 ggcggcggtc agtccggcgg cgtggtgttc gccttttccc acgtcgatgc cgcaaaacac 
      961 ggcgtaactc gtgtccattc agcagtcctt cgtccggcca gtccggtcgc cgatccagca 
     1021 tcgatacccg acacccacgt tacgaggtga cctaccgcag tttccggtgg tcctgtccct 
     1081 atcagcggtc catcgacgcc accaggttcg gcgacaccac ccccggatca ttcgagagac 
     1141 aggggcaaag aagtcatacc gaagatggcg accgcagtcc cgcgaaacgg gaccacaaag 
     1201 acggtaacgg caaaccggcc tgggaacccg cgggtttcgc caccctcgaa cgggtcctgg 
     1261 cgacgagctg accgcgtcgc ggcgcccgat aagagaccac tcgagctccg accctgattc 
     1321 tgtgcgatag tgagttatgc aaccaaatga cgtggctttg ttgaataaat cgaacttttg 
     1381 ctgagttgaa ggatcagctc gatataaacg cagaaaggcc cacccgaagg tgagccagtg 
     1441 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc 
     1501 gactgagcct ttcgttttat ttgatgcctg gttatcatga gaccggtctc aattcgcagc 
     1561 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac 
     1621 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg 
     1681 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc 
     1741 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt 
     1801 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg 
     1861 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag 
     1921 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa 
     1981 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgcagctt 
     2041 catgtggtcg gggtagcggg cgaagcattg caggccgtag ccgaaggtgg tcacgagggt 
     2101 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta 
     2161 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc 
     2221 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatcta 
     2281 gtatttctcc tctttctcta gtatgtgtga aattgttatc cgcttctaag caaaccttaa 
     2341 cagcagagaa ggctagcaca atacctagga ctgagctagc tgtcaactcg aggtgaagac 
     2401 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 
     2461 agacgtcgat atctggcgaa aatggttaat caagggcact ctcgtattga ccaccctgga 
     2521 ggagatgatc gaggatggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 
     2581 cggattagaa aaacaactta aatgtgaaag tgggtcttaa tgaccatggg ctagcggctt 
     2641 tagctaatta attggggacc ctagaggtcc ccttttttat tttgcggccg ccgggggccg 
     2701 ggggcggcgc cgggcggccc ggggcgtcag gcgccggggg cggtgtccgg cggcccccag 
     2761 aggaactgcg ccagttcctc cggatcggtg aagccggaga gatccagcgg ggtctcctcg 
     2821 aacacctcga agtcgtgcag gaaggtgaag gcgagcagtt cgcgggcgaa gtcctcggtc 
     2881 cgcttccact gcgccccgtc gagcagcgcg gccaggatct cgcggtcgcc ccggaaggcg 
     2941 ttgagatgca gttgcaccag gctgtagcgg gagtctcccg catagacgtc ggtgaagtcg 
     3001 acgatcccgg tgacctcggt cgcggccagg tccacgaaga tgttggtccc gtgcaggtcg 
     3061 ccgtggacga accggggttc gcggccggcc agcagcgtgt ccacgtccgg cagccagtcc 
     3121 tccaggcggt ccagcagccg gggcgagagg tagccccacc cgcggtggtc ctcgacggtc 
     3181 gccgcgcggc gttcccgcag cagttccggg aagacctcgg aatggggggt gagcacggtg 
     3241 ttcccggtca gcggcaccct gtgcagccgg ccgagcaccc ggccgagttc gcgggccagg 
     3301 gcgagcagcg cgttccggtc ggtcgtgccg tccatcgcgg accgccaggt ggtgccggtc 
     3361 atccggctca tcaccaggta gggccacggc caggctccgg tgccgggccg cagctcgccg 
     3421 cggccgagga ggcggggcac cggcaccggg gcgtccgcca ggaccgcgta cgcctccgac 
     3481 tccgacgcga ggctctccgg accgcaccag tgctcgccga acagcttgat caccgggtcg 
     3541 ggctcgccga ccagtacggg gttggtgctc tcgccgggca cccgcagcac cggcggcacc 
     3601 ggcagcccga gctcctccag ggctcggcgg gccagcggct cccagaattc ctggtcgttc 
     3661 cgcaggctcg cgtaggaatc atccgaatca atacggtcga gaagtaacag ggattcttgt 
     3721 gtcacagcgg acctctattc ttgggtcgga tagtgtcgag aactgcgcgg tcatggtatt 
     3781 cgggacgttt gagtgccgag caagcggctc gcatcactcg gtggcttccc ggtgcccgcc 
     3841 tgtgtgctga catgctttcc agtgctcgag tttgtcgcca aacatcgagc cggagatgaa 
     3901 gaacagatca tgtaattcaa tctcttcgcc gagacgggtg acgaggcgat tgacctcgtc 
     3961 acgaggtact tcgtcaacga gtttcgatga gctgtgatag tgctgccagg tgctgcgcat 
     4021 ctcgtcgagc ccatacgtca cttcggtcgg gtcgttcagc ccggtccttg cagttgccca 
     4081 caagcagtgg ttctccacga tccacgctca gccgccgacg ccgccgtgca gcgttggcat 
     4141 acgtgcttcg tgcagtagct gttcgtccgt gtctgccggc cactcggtgg tgccgccgac 
     4201 gcgggaccat tgcgcgatca ggcatgagaa ggtgctct 
//
