LOCUS       pDD103                  4769 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12540740|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    4697..4769
                     /vntifkey="21"
                     /label=UTR\+\RBS
     misc_feature    4685..4690
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-10\site
     misc_feature    4662..4665
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-35\site
     misc_feature    4656..4696
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                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=Constitutive\Promoter,\highest\activity
     misc_feature    1781..2779
                     /ApEinfo_fwdcolor=#ff6fcf
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=KanR\cassette
     misc_feature    3015..4284
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pAL5000
     terminator      4474..4501
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     misc_feature    894..1513
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pMB1\ori
     CDS             1..711
                     /ApEinfo_revcolor=#f6989d
                     /ApEinfo_fwdcolor=#f6989d
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT      956 a      1457 c      1400 g       956 t 
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta acctgaattc 
      721 gagctggatc cagcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
      781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     1081 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     1321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttggg gtgggcgaag 
     1621 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 
     1681 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 
     1741 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa 
     1801 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 
     1861 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 
     1921 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 
     1981 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg 
     2041 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 
     2101 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 
     2161 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 
     2221 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 
     2281 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 
     2341 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt 
     2401 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 
     2461 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 
     2521 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 
     2581 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg 
     2641 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag 
     2701 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa 
     2761 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc 
     2821 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc 
     2881 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct agcgtctacc 
     2941 aggactttta cctgtccgac ccgttgcaac ggaacccccc acggaacccc cgcgacaccc 
     3001 gctccccaat tgcgttagaa cagcggtgga ttgtcggctt cgttgtgggc cttttgagcc 
     3061 gcttcctgtt ctgccgcacg ctctttcctc gcccgatagc cgagtcgctt aacggtgtcc 
     3121 agatgcagcc cgaaatgttt ggccgtttgc ggccaagagt ggccctcgtc gtcgtgatag 
     3181 gcgcggatgc gttcgcggcg tgcagcctgc tcggcgagcc actcgctgcg ttcctgcgcc 
     3241 acgagccgga cgacgtggcg ttcggatagt ccggtgattc gagcgccttc ggcggcggtc 
     3301 acgcgccgct ttttgcggac agtcggctgc cggttgtagc cgtcgctgta gccgtcgctc 
     3361 atagcaatgc ctccatggct gacgcggact ttgcgcgccg cgcaactgtg ctcgccgccg 
     3421 tgcgcgctgc tgcgcccttc cgcgagatgg ccgactggcg cgcactgagt gtggcctcgt 
     3481 agaccacgat cccgtccgcc caaatgcgcg acttggttgt gatccaacgc caaatgctgt 
     3541 tggcgatggc gcggacctcg ctgtccggta gcggtccggg acacacgtcg ttgcacggga 
     3601 actcggcgtt tcgcgcgtgg cactcggcat agatcgcgcg gccgagtccg tccacgttcc 
     3661 gggtcggcag gtagatccgc atgagggcgg gacgataggc ccacaacctg acggaatcga 
     3721 acagtgcgca attccgccct agcggcgtcg gagccgcttt gtacgtggtc tgctgacgcc 
     3781 agcgcggcgg tggcatgttc gcgccgagct cggcctcgat gtggctgagt gtgtagagat 
     3841 ctgagtggag ccattccgtt tcccaggcga tgtggccggg gtttttggtc atgaggcctg 
     3901 agtaactgcg gtcgccgtcc acggcgcgcc gaaggccttc ggcgcacgcc gccatgtatg 
     3961 cgagcggctt acgccgcgcg tattcggtgc gtggaacagg ggcgttgagt gcccacactg 
     4021 cgtgtgcgtg gccgttggcg cgattgccca cgatcgcgtt gggcagcgga tgggaccccc 
     4081 gggcgctgag cgctcggagc gctgcgtctg gatggtctac gtccacgacc agcaggtttg 
     4141 ccagcgctgt tgggttcgcc tcgatgtacc ggcggcctag ggccgacgcg cggctttggc 
     4201 ggtagatccc ctcgagcaga tcgtcgcttg ccagcggcca gtacggcagc cagagctgct 
     4261 caaattcgtc ggcgacgtgg ctcacgcttg gtagtagacc acgattaatc accggtgtat 
     4321 ggtccgacac gagctccaag tcagatattt cgctgagggg ccaccccaca actgcacact 
     4381 cccccgctct cccgtcgagc cctggtggtg gaacaccagc gacagccgag cacccccaac 
     4441 cacctgtacc aaccactagt tgattagcta agcagaaggc catcctgacg gatggccttt 
     4501 ttgcgtttaa tactgtttaa actctagaaa tattggatcg tcggcaccgt cacggccgtg 
     4561 ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag 
     4621 ctctacgcca tcccggcgtt gatctgtgcg ttcgctgtgc gggctctaac acgtcctagt 
     4681 atggtaggat gagcaacatt tcgacgccga gagattcgcc gcccgaaatg agcacgatcc 
     4741 gcatgcttaa ttaagaagga gatatacat 
//
