LOCUS       pDD112                  5021 bp    DNA     circular      5-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12540807|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    366..438
                     /vntifkey="21"
                     /label=UTR\+\RBS
     misc_feature    354..359
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-10\site
     misc_feature    331..334
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=-35\site
     misc_feature    325..365
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=pConstitutive
     terminator      143..170
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     misc_feature    1106..1177
                     /vntifkey="21"
                     /label=rnnB\Term
     exon            1413..2537
                     /vntifkey="61"
                     /label=GentR
     misc_feature    2859..4706
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=#996633
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=NG2\ori
     misc_feature    439..1177
                     /vntifkey="21"
                     /label=CM\(opt)
BASE COUNT     1192 a      1427 c      1335 g      1067 t 
ORIGIN
        1 agctccaagt cagatatttc gctgaggggc caccccacaa ctgcacactc ccccgctctc 
       61 ccgtcgagcc ctggtggtgg aacaccagcg acagccgagc acccccaacc acctgtacca 
      121 accactagtt gattagctaa gcagaaggcc atcctgacgg atggcctttt tgcgtttaat 
      181 actgtttaaa ctctagaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac 
      241 gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat 
      301 cccggcgttg atctgtgcgt tcgctgtgcg ggctctaaca cgtcctagta tggtaggatg 
      361 agcaacattt cgacgccgag agattcgccg cccgaaatga gcacgatccg catgcttaat 
      421 taagaaggag atatacatat ggagaaaaag atcacggggt acaccaccgt ggacatcagc 
      481 cagtggcacc gtaaggaaca tttcgaagcg ttccagtccg tcgcgcaatg tacgtacaat 
      541 cagaccgtcc aactcgatat tacggcgttt ctgaagaccg tgaagaagaa taagcacaaa 
      601 ttttaccccg cctttattca tatcctggcg cggctgatga acgcccatcc agaatttcgc 
      661 atggccatga aagatggcga gttggtcatc tgggatagcg tccatccatg ttatacggtg 
      721 ttccacgagc agaccgaaac cttttcgagc ttgtggagcg agtatcacga tgatttccgg 
      781 cagttcttgc acatttatag ccaagatgtg gcctgctatg gtgagaatct ggcctacttt 
      841 ccgaaaggtt tcattgagaa catgtttttt gtgtccgcca atccgtgggt ctcgttcacg 
      901 agcttcgacc tcaacgtcgc gaatatggat aacttttttg cgccggtctt taccatgggg 
      961 aaatattata cgcagggcga taaggtgctg atgccactcg cgatccaagt gcatcatgcc 
     1021 gtctgcgatg gcttccacgt cggtcggatg ttgaacgaac tccaacaata ttgcgatgag 
     1081 tggcaaggtg gtgcgtaagc tatgccaaat aaaacgaaag gctcagtcga aagactgggc 
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctga ggactgtctt ctttatcatg 
     1201 caactcgtag gacaggtgcc acttcatccg gggtcagcac caccggcaag cgccgcgacg 
     1261 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga 
     1321 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg 
     1381 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac 
     1441 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt 
     1501 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 
     1561 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc 
     1621 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 
     1681 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg 
     1741 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg 
     1801 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg 
     1861 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag 
     1921 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg 
     1981 agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg 
     2041 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc 
     2101 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag 
     2161 aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga 
     2221 tcggcttccc ggccgcggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc 
     2281 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata 
     2341 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg 
     2401 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata 
     2461 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta 
     2521 tgcagcggaa aagcgctgct tccctgctgt tttgtgggcc gcaaagcgct ccggcttaac 
     2581 tcaggcgtta gtcccatcac ccgctttatg cgcgcagcca cttatttcag cgctccgccc 
     2641 tgttccaaag gctttctagc cttttgggcc tccagcgctt gtgcagcaag cgccaccagc 
     2701 tatccttctt gcagcgctgc gccgctttcg tggtccagcg gtttttcttg ggctgcgccc 
     2761 ttcttcaagg ccgggcgctc ccttttggcc ttcgggtcta actctgcggt caagcggacc 
     2821 cgcattctgc gggccgctta ccttggccgt tagacatcat ggtaaatctg cgcagacagc 
     2881 cctgtgcagc tgaaacgcgg ttacgtatag cttgccatat gtctagccat acgtaaccgc 
     2941 aggtaaaagg catatttttc gcgtgtcatg gctagtaaat aacaccggtg tcatttagag 
     3001 tcagggaaag acaatgaaaa acgaagaaag ccaccgggcg gcaacccgat gactttcgct 
     3061 tatcacccag cacacacctg ggagaaatca cggtcatgag tttacagact catgcgcaga 
     3121 atgcgcacac taaaacacct acccgcgtcg agcgcgaccg tggtggactg gacaacaccc 
     3181 cagcatctgc cagtgaccgc gaccttttac gcgatcatct aggccgcgat gtactccacg 
     3241 gttcagtcac acgagacttt aaaaaggcct atcgacgcaa cgctgacggc acgaactcgc 
     3301 cgcgtatgta tcgcttcgag actgatgctt taggacggtg cgagtacgcc atgctcacca 
     3361 ccaagcagta cgccgccgtc ctggtcgtag acgttgacca agtaggtacc gcaggcggtg 
     3421 accccgcaga cttaaacccg tacgtccgcg acgtggtgcg ctcactgatt actcatagcg 
     3481 tcgggccagc ctgggtgggt attaacccaa ctaacggcaa agcccagttc atatggctta 
     3541 ttgaccctgt ctacgctgac cgtaacggta aatctgcgca gatgaagctt cttgcagcaa 
     3601 ccacgcgtgt gctgggtgag cttttagacc atgacccgca cttttcccac cgctttagcc 
     3661 gcaacccgtt ctacacaggc aaagccccta ccgcttatcg ttggtatagg cagcacaacc 
     3721 gggtgatgcg ccttggagac ttgataaagc aggtaaggga tatggcagga cacgaccagt 
     3781 tcaaccccac cccacgccag caattcagct ctggccgcga acttatcaac gcggtcaaga 
     3841 cccgccgtga agaagcccaa gcattcaaag cactcgccca ggacgtagac gcggaaatcg 
     3901 ccggtggtct cgaccagtat gacccggaac ttatcgacgg tgtgcgtgtg ctctggattg 
     3961 tccaaggaac cgcagcacgc gacgaaacag cctttagaca tgcgcttaag actggccacc 
     4021 gcttgcgcca gcaaggccaa cgcctgacag acgcagcaat catcgacgcc tatgagcacg 
     4081 cctacaacgt cgcacacacc cacggcggtg caggccgcga caacgagatg ccacccatgc 
     4141 gcgaccgcca aaccatggca aggcgcgtgc gcgggtatgt cgcccaatcc aagagcgaga 
     4201 cctacagcgg ctctaacgca ccaggtaaag ccaccagcag cgagcggaaa gccttggcca 
     4261 cgatgggacg cagaggcgga caaaaagccg cacaacgctg gaaaacagac cccgagggca 
     4321 aatatgcgca agcacaaagg tcgaagcttg aaaagacgca ccgtaagaaa aaggctcaag 
     4381 gacgatctac gaagtcccgt attagccaaa tggtgaacga tcagtatttc cagacaggga 
     4441 cagttcccac gtgggctgaa ataggggcag aggtaggagt ctctcgcgcc acggttgcta 
     4501 ggcatgtcgc ggagctaaag aagagcggtg actatccgga cgtttaaggg gtctcatacc 
     4561 gtaagcaata tacggttccc ctgccgttag gcagttagat aaaacctcac ttgaagaaaa 
     4621 ccttgagggg cagggcagct tatatgcttc aaagcatgac ttcctctgtt ctcctagacc 
     4681 tcgcaaccct ccgccataac ctcaccgaat tgcttaagtt tcaccccttt gacggcttaa 
     4741 ccgccgcata ggaaatgccc tccggactaa ttgtctggag ggcatttttg ctacctgctg 
     4801 tgcactttgt acataaggca acaagatttt ggcaaaaggt ggaagggcga attgctgcgc 
     4861 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca 
     4921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 
     4981 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac g 
//
