LOCUS       226_epB_UbC_CymR        6598 bp ds-DNA     circular     06-NOV-2018
DEFINITION  epB-003_epB-CAG-EGFP (6269 bp).
ACCESSION   .
VERSION     .
KEYWORDS    epB-003_epB-CAG-EGFP-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2015 from SnapGene Viewer 1.5.3
            http://www.snapgene.com
COMMENT     Sequencing from 2018-10-09 and 2018-10-10.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(2573..2659,2661..3791)
                     /locus_tag="UbC Promoter"
                     /label="UbC Promoter"
                     /ApEinfo_label="UbC Promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5401..5409
                     /locus_tag="Destablization Element"
                     /label="Destablization Element"
                     /ApEinfo_label="Destablization Element"
                     /ApEinfo_fwdcolor="#c0ff7b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3891..4499
                     /locus_tag="CymR"
                     /label="CymR"
                     /ApEinfo_label="CymR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1084..1766
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6158..6464
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             327..986
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5679..5932
                     /locus_tag="ePiggyBac 3'TR"
                     /label="ePiggyBac 3'TR"
                     /ApEinfo_label="ePiggyBac 3'TR"
                     /ApEinfo_fwdcolor="#fbff3e"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4620..5336
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#0cff0e"
                     /ApEinfo_revcolor="#0cff0e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2223..2548
                     /locus_tag="ePiggyBac 5'TR"
                     /label="ePiggyBac 5'TR"
                     /ApEinfo_label="ePiggyBac 5'TR"
                     /ApEinfo_fwdcolor="#f6ff57"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5415..5423
                     /locus_tag="Destablization Element(1)"
                     /label="Destablization Element(1)"
                     /ApEinfo_label="Destablization Element"
                     /ApEinfo_fwdcolor="#acff71"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5371..5394
                     /locus_tag="Gateway attB2"
                     /label="Gateway attB2"
                     /ApEinfo_label="Gateway attB2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3806..3829
                     /locus_tag="Gateway attB1"
                     /label="Gateway attB1"
                     /ApEinfo_label="Gateway attB1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5436..5673
                     /locus_tag="SV40 polyA"
                     /label="SV40 polyA"
                     /ApEinfo_label="SV40 polyA"
                     /ApEinfo_fwdcolor="#da46ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggggcgc
     2221 gccgcagcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     2281 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     2341 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     2401 actataacaa ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     2461 ttaactcata cgataattat attgttattt cgtgttctac ttacgtgata acttattata
     2521 tatatatttt cttgttatag atatccttct tcgacattga ttattgacta gagatctggc
     2581 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2641 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2701 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2761 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2821 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2881 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2941 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
     3001 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
     3061 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
     3121 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     3181 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     3241 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     3301 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     3361 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     3421 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     3481 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     3541 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     3601 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     3661 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     3721 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctgGCCGTTT TTGGCTTTTT
     3781 TGTTAGACGA AGCTCGAGGA tatcaCAAGT TTGTACAAAA AAGCAGGCTT TAAAGGAACC
     3841 AATTCAGTCG ACTGGATCCG GTACCGAATT CGCGGCCGCC TAGCTAAACC ATGTCACCTA
     3901 AGAGAAGAAC TCAGGCAGAG AGAGCAATGG AAACTCAGGG GAAACTCATC GCAGCCGCAC
     3961 TCGGGGTCCT CAGAGAAAAG GGCTACGCAG GATTCCGGAT CGCCGATGTG CCAGGAGCAG
     4021 CTGGTGTCTC ACGCGGGGCT CAGAGCCACC ATTTCCCTAC CAAACTGGAG CTGCTCCTGG
     4081 CAACATTTGA GTGGCTGTAT GAACAGATCA CTGAGAGGAG CAGAGCCCGG CTCGCTAAGC
     4141 TGAAACCAGA GGACGATGTG ATTCAGCAGA TGCTCGACGA TGCAGCCGAA TTCTTTCTGG
     4201 ACGATGACTT CAGCATCTCC CTCGATCTGA TTGTGGCTGC AGATCGGGAC CCCGCCCTGC
     4261 GAGAAGGGAT TCAGCGAACC GTGGAGCGCA ACCGATTTGT GGTCGAAGAT ATGTGGCTCG
     4321 GCGTGCTGGT GTCTAGGGGA CTGAGTAGAG ATGACGCCGA GGACATCCTC TGGCTGATTT
     4381 TCAATTCCGT GAGGGGCCTC GCTGTCAGAT CTCTGTGGCA GAAGGACAAA GAGCGGTTTG
     4441 AACGAGTCAG AAACAGCACA CTGGAAATCG CACGGGAGAG ATACGCTAAG TTCAAAAGGG
     4501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCCGGATAAG GCACGTCGTC
     4561 CAGCAGTAAG ATCACCACCA CTAGGAACTC GTAAGCGAAA GCGTCCTTCT CGGGGTCTGA
     4621 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
     4681 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
     4741 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
     4801 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
     4861 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
     4921 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
     4981 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
     5041 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
     5101 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
     5161 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
     5221 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
     5281 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG
     5341 TGCCTAGGCG GCCGCACTCG AGATATCTAG ACCCAGCTTT CTTGTACAAA GTGGTGATCC
     5401 TTATTTATTG ATCCTTATTT ATTGATCTGC GGCCGCGACT CTAGATCATA ATCAGCCATA
     5461 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
     5521 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
     5581 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
     5641 GTGGTTTGTC CAAACTCATC AATGTATCTT AAGGaattcg ataaaagttt tgttacttta
     5701 tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt
     5761 tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat
     5821 taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt
     5881 atctttaacg tacgtcacaa tatgattatc tttctagggt taatctagct gcggcgcgcc
     5941 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6001 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6061 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6121 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6481 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttag
//
