LOCUS       Exported                7291 bp ds-DNA     circular SYN 10-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             949..2889
                     /label=AKAR5.1 open ended (EGFPdelta/cp-sREACh version)
     misc_recomb     949..1632
                     /label=mEGFPdelta
     CDS             1642..2061
                     /label=FHA domain
     misc_feature    2107..2130
                     /label=PKA substrate
     misc_signal     2155..2889
                     /label=cp-sREACh E172 (@end), no D173
     CDS             2911..3507
                     /label=MTBD (MAP2c microtubule binding domain)
     misc_feature    3007..3096
                     /label=MT binding repeat1
     misc_feature    3097..3189
                     /label=MT binding repeat2
     misc_feature    3190..3285
                     /label=MT binding repeat3
     CDS             3286..3507
                     /codon_start=1
                     /label=C-terminal acidic region of MAP2
                     /translation="NVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVS
                     SSGSINLLESPQLATLAEDVTAALAKQGL"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcccgggc tcgagaccgg tcgccaccat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg cccgcatgca taagttttct caagaacaga tcggcgaaaa cattgtgtgc
     1681 agggtcattt gtaccacggg tcaaattccc atccgagatt tgtcagctga tatttcacaa
     1741 gtgcttaagg aaaaacgatc cataaagaaa gtttggacat ttggtagaaa cccagcctgt
     1801 gactatcatt taggaaacat ttcaagactg tcaaataagc atttccaaat actactagga
     1861 gaagacggta accttttatt gaatgacatt tccactaatg ggacctggtt aaatgggcaa
     1921 aaagtcgaga agaacagcaa tcagttactg tctcaaggtg atgaaataac cgttggtgta
     1981 ggcgtggaat cagatatttt atctctggtc attttcataa acgacaaatt taagcagtgc
     2041 ctcgagcaga acaaagttga tcgctctgca ggtaagccag gcagcggcga gggcagcacc
     2101 aagggcctgc gtcgcgccac cctggtggac ggcggcaccg gcggcagcga gctcatgggc
     2161 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2221 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     2281 cgcgatcaca tggtcctgct ggagagagtg accgccgccg ggatcactct cggcatggac
     2341 gagctataca agggtggaac tggaggttca atggtgagca agggcgagga gctgttcacc
     2401 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2461 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc
     2521 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     2581 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2641 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2701 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2761 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactggaa cagcgtcaac
     2821 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2881 aacatcgagt ccggagtgta caagggtacc ccaggaaccc ccaagtctgc catcttggtg
     2941 ccgagtgaga agaaggtcgc catcatacgt actcctccaa aatctcctgc gactcccaag
     3001 cagcttcggc ttattaacca accactgcca gacctgaaga atgtcaaatc caaaatcgga
     3061 tcaacagaca acatcaaata ccagcctaaa ggggggcagg tacaaattgt taccaagaaa
     3121 atagacctaa gccatgtgac atccaaatgt ggctctctga agaacatccg ccacaggcca
     3181 ggtggcggac gtgtgaaaat tgagagtgta aaactagatt tcaaagaaaa ggcccaagct
     3241 aaagttggtt ctcttgataa tgctcatcat gtacctggag gtggtaatgt caagattgac
     3301 agccaaaagt tgaacttcag agagcatgct aaagcccgtg tggaccatgg ggctgagatc
     3361 attacacagt ccccaggcag atccagcgtg gcatcacccc gacgactcag caatgtctcc
     3421 tcgtctggaa gcatcaacct gctcgaatct cctcagcttg ccactttggc tgaggatgtc
     3481 actgctgcac tcgctaagca gggcttgtaa tctagagtcg acctgcagaa gcttggccgc
     3541 catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3661 atgtatctta tcatgtctgg atcgatcggg aattaattcg gcgcagcacc atggcctgaa
     3721 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
     3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3901 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4081 ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg gcactggccg
     4141 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4201 cacatccccc cttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4261 aacagttgcg tagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4321 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4561 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4681 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4801 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4861 aagccaactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4921 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4981 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     5041 cagtattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5461 tttaaagttc tgctatgtgg cgcggtatta tcccgtgatg acgccgggca agagcaactc
     5521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5761 gccataccaa acgacgagcg tgacaccacg atgccagcag caatggcaac aacgttgcgc
     5821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6421 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6661 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7021 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7081 cccgcgcgtt ggccgattca ttaatccagc tggcacgaca ggtttcccga ctggaaagcg
     7141 ggcagtgagc gcaacgcaat taatgtgagt tacctcactc attaggcacc ccaggcttta
     7201 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7261 ggaaacagct atgaccatga ttacgaatta a
//
