LOCUS       Exported                6748 bp ds-DNA     circular SYN 27-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    931..987
                     /label=NES from PKI alpha
     CDS             1000..2940
                     /label=AKAR5.1 (EGFPdelta/cp-sREACh version)
     misc_recomb     1000..1683
                     /label=mEGFPdelta
     misc_feature    1618..1620
                     /label=A206K (monomeric mutation)
     misc_feature    2170..2172
                     /label=PKA phospho site
     misc_signal     2206..2940
                     /label=cp-sREACh @ E172, note no D173.
     misc_feature    2311..2313
                     /label=S208
     misc_feature    2356..2361
                     /label=R223,V224
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgccacc atgctgcaga acgagcttgc tcttaagttg
      961 gctggacttg atattaacaa gactggaccg gtcgccacca tggtgagcaa gggcgaggag
     1021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gcccgcatgc ataagttttc tcaagaacag atcggcgaaa acattgtgtg cagggtcatt
     1741 tgtaccacgg gtcaaattcc catccgagat ttgtcagctg atatttcaca agtgcttaag
     1801 gaaaaacgat ccataaagaa agtttggaca tttggtagaa acccagcctg tgactatcat
     1861 ttaggaaaca tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt
     1921 aaccttttat tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag
     1981 aagaacagca atcagttact gtctcaaggt gatgaaataa ccgttggtgt aggcgtggaa
     2041 tcagatattt tatctctggt cattttcata aacgacaaat ttaagcagtg cctcgagcag
     2101 aacaaagttg atcgctctgc aggtaagcca ggcagcggcg agggcagcac caagggcctg
     2161 cgtcgcgcca ccctggtgga cggcggcacc ggcggcagcg agctcatggg cagcgtgcag
     2221 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2281 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2341 atggtcctgc tggagagagt gaccgccgcc gggatcactc tcggcatgga cgagctatac
     2401 aagggtggaa ctggaggttc aatggtgagc aagggcgagg agctgttcac cggggtggtg
     2461 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2521 ggcgagggcg atgccaccta cggcaagctg accctgaagc tcatctgcac caccggcaag
     2581 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat gtgcttcgcc
     2641 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2701 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2761 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2821 gacggcaaca tcctggggca caagctggag tacaactgga acagcgtcaa cgtctatatc
     2881 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2941 taagcggccg caattccccg gggatcctct agagtcgacc tgcagaagct tggccgccat
     3001 ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3121 tatcttatca tgtctggatc gatcgggaat taattcggcg cagcaccatg gcctgaaata
     3181 acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat
     3241 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3301 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3361 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3421 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3481 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3541 ggcttttttg gaggcctagg cttttgcaaa aagctgttaa cagcttggca ctggccgtcg
     3601 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     3661 atcccccctt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3721 agttgcgtag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     3781 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3901 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3961 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4021 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4081 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4141 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4201 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4261 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4321 ccaactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     4381 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4441 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     4501 tattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4561 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4681 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4741 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4801 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4861 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4921 aaagttctgc tatgtggcgc ggtattatcc cgtgatgacg ccgggcaaga gcaactcggt
     4981 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5041 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5101 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5161 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5221 ataccaaacg acgagcgtga caccacgatg ccagcagcaa tggcaacaac gttgcgcaaa
     5281 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5341 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5401 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5461 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5521 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5581 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5641 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5881 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6121 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6481 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6541 gcgcgttggc cgattcatta atccagctgg cacgacaggt ttcccgactg gaaagcgggc
     6601 agtgagcgca acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac
     6661 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6721 aacagctatg accatgatta cgaattaa
//
