LOCUS Exported 6781 bp ds-DNA circular SYN 31-AUG-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6781) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 6, 2018 from SnapGene 4.2.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6781 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 949..2943 /label=AKAR5.1-KRas-C CDS 949..2889 /label=AKAR5.1 (EGFPdelta/cp-sREACh version) misc_recomb 949..1632 /label=mEGFPdelta misc_feature 2899..2943 /label=KRas-C ORIGIN 1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 901 acctcggttc tatcgattga attcccgggc tcgagaccgg tcgccaccat ggtgagcaag 961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 1561 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1621 gtgaccgccg cccgcatgca taagttttct caagaacaga tcggcgaaaa cattgtgtgc 1681 agggtcattt gtaccacggg tcaaattccc atccgagatt tgtcagctga tatttcacaa 1741 gtgcttaagg aaaaacgatc cataaagaaa gtttggacat ttggtagaaa cccagcctgt 1801 gactatcatt taggaaacat ttcaagactg tcaaataagc atttccaaat actactagga 1861 gaagacggta accttttatt gaatgacatt tccactaatg ggacctggtt aaatgggcaa 1921 aaagtcgaga agaacagcaa tcagttactg tctcaaggtg atgaaataac cgttggtgta 1981 ggcgtggaat cagatatttt atctctggtc attttcataa acgacaaatt taagcagtgc 2041 ctcgagcaga acaaagttga tcgctctgca ggtaagccag gcagcggcga gggcagcacc 2101 aagggcctgc gtcgcgccac cctggtggac ggcggcaccg gcggcagcga gctcatgggc 2161 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 2221 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 2281 cgcgatcaca tggtcctgct ggagagagtg accgccgccg ggatcactct cggcatggac 2341 gagctataca agggtggaac tggaggttca atggtgagca agggcgagga gctgttcacc 2401 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 2461 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc 2521 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg 2581 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2641 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 2701 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 2761 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactggaa cagcgtcaac 2821 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 2881 aacatcgagg gtgaattcgg taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt 2941 atgtaatcta ggggatccac cggaagccac ctgcatgcgg ccgcaattcc ccggggatcc 3001 tctagagtcg acctgcagaa gcttggccgc catggcccaa cttgtttatt gcagcttata 3061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 3121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgatcggg 3181 aattaattcg gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt 3241 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 3301 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 3361 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3421 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 3481 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 3541 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 3601 aaaaagctgt taacagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc 3661 ctggcgttac ccaacttaat cgccttgcag cacatccccc cttcgccagc tggcgtaata 3721 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg tagcctgaat ggcgaatggc 3781 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa 3841 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 4021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc 4081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc 4201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4261 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct 4321 cagtacaatc tgctctgatg ccgcatagtt aagccaactc cgctatcgct acgtgactgg 4381 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 4441 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 4501 ttttcaccgt catcaccgaa acgcgcgagg cagtattctt gaagacgaaa gggcctcgtg 4561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 4621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 4681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 4741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 4801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 4861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 4921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 4981 tcccgtgatg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 5041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 5101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 5161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 5221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 5281 atgccagcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 5341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 5401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 5461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 5521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 5581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 5641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc 6181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 6481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 6541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatccagc 6601 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 6661 tacctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 6721 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaatta 6781 a //