LOCUS       Exported                6754 bp ds-DNA     circular SYN 31-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             934..2946
                     /label=lifeact-AKAR5.1
     misc_feature    934..984
                     /label=lifeact
     misc_feature    1006..2946
                     /label=AKAR5.1: AKAR5 cp-sREACh variant w. EGFPdelta
     misc_feature    1006..1689
                     /label=mEGFPdelta
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtcgcc accatgggcg tggccgactt gatcaagaag
      961 ttcgagtcca tctccaagga ggagggggat ccaccggtcg ccaccatggt gagcaagggc
     1021 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1081 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1141 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1201 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1261 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1321 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1381 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1441 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1501 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1621 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1681 accgccgccc gcatgcataa gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg
     1741 gtcatttgta ccacgggtca aattcccatc cgagatttgt cagctgatat ttcacaagtg
     1801 cttaaggaaa aacgatccat aaagaaagtt tggacatttg gtagaaaccc agcctgtgac
     1861 tatcatttag gaaacatttc aagactgtca aataagcatt tccaaatact actaggagaa
     1921 gacggtaacc ttttattgaa tgacatttcc actaatggga cctggttaaa tgggcaaaaa
     1981 gtcgagaaga acagcaatca gttactgtct caaggtgatg aaataaccgt tggtgtaggc
     2041 gtggaatcag atattttatc tctggtcatt ttcataaacg acaaatttaa gcagtgcctc
     2101 gagcagaaca aagttgatcg ctctgcaggt aagccaggca gcggcgaggg cagcaccaag
     2161 ggcctgcgtc gcgccaccct ggtggacggc ggcaccggcg gcagcgagct catgggcagc
     2221 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2281 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     2341 gatcacatgg tcctgctgga gagagtgacc gccgccggga tcactctcgg catggacgag
     2401 ctatacaagg gtggaactgg aggttcaatg gtgagcaagg gcgaggagct gttcaccggg
     2461 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2521 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctcat ctgcaccacc
     2581 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc
     2641 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2701 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2761 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2821 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actggaacag cgtcaacgtc
     2881 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2941 atcgagtaag cggccgcaat tccccgggga tcctctagag tcgacctgca gaagcttggc
     3001 cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3121 tcaatgtatc ttatcatgtc tggatcgatc gggaattaat tcggcgcagc accatggcct
     3181 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     3241 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3301 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3541 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg
     3601 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3661 cagcacatcc ccccttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3721 cccaacagtt gcgtagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     3781 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     3841 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3901 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3961 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4021 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4081 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4141 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4201 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4261 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     4321 gttaagccaa ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     4381 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4441 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4501 aggcagtatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4561 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4921 acttttaaag ttctgctatg tggcgcggta ttatcccgtg atgacgccgg gcaagagcaa
     4981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5221 gaagccatac caaacgacga gcgtgacacc acgatgccag cagcaatggc aacaacgttg
     5281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6121 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6421 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6481 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6541 ctccccgcgc gttggccgat tcattaatcc agctggcacg acaggtttcc cgactggaaa
     6601 gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc accccaggct
     6661 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6721 acaggaaaca gctatgacca tgattacgaa ttaa
//
