LOCUS       Exported                8935 bp ds-DNA     circular SYN 15-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             982..3153
                     /label=PSD-95
     misc_feature    1009..1173
                     /label=PEST domain
     misc_feature    1174..1428
                     /label=PDZ1 (by NCBI domain search)
     misc_feature    1459..1713
                     /label=PDZ2 (by NCBI conserved domain search)
     misc_feature    1714..1917
                     /label=PDZ-associated domain (unknown function)
     misc_feature    1912..2148
                     /label=PDZ3
     misc_feature    1962
                     /label=actually a T here (silent)
     misc_feature    2269..2466
                     /label=SH3
     misc_feature    2578..3114
                     /label=Guanylate kinase domain
     misc_feature    3079..3153
                     /label=remained in PDZ1-2 construct
     misc_feature    3187..5127
                     /label=AKAR5.1
     misc_feature    3187..3870
                     /label=mEGFPdelta
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgattaa ctccaatgaa gtcagagccc cctactcgcc
      961 gccgcggcca ggccccccaa catggactgt ctctgtatag tgacaaccaa gaaataccgc
     1021 taccaagatg aagacacgcc ccctctggaa cacagcccgg cccacctccc caaccaggcc
     1081 aattctcccc ctgtgattgt caacacggac accctagaag ccccaggata tgagttgcag
     1141 gtgaatggaa cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca
     1201 ggtctgggct tcagcatcgc aggtggcact gacaacccgc acatcggtga cgacccgtcc
     1261 atttttatca ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc
     1321 aatgacagca tcctgtttgt aaatgaagtg gatgttcggg aggtgaccca ttcagctgcg
     1381 gtggaggccc tcaaagaggc aggttccatc gttcgcctct atgtcatgcg ccggaaaccc
     1441 ccagccgaaa aggtcatgga gatcaaactc atcaaagggc ctaaaggact tggcttcagc
     1501 attgcggggg gcgttgggaa ccagcacatc cctggagata acagcatcta tgtaacgaag
     1561 atcatcgaag gaggtgctgc ccacaaggat ggcaggttgc agattggaga caagatcctg
     1621 gcggtcaaca gtgtggggct ggaggacgtc atgcacgagg atgccgtggc agccctgaag
     1681 aacacatatg acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac
     1741 agctatgctc ccccagacat cacaacctcg tattctcagc acctggacaa tgagatcagt
     1801 catagcagct acttgggcac tgactacccc acagccatga cccccacttc ccctcggcgc
     1861 tactcccctg tggccaagga cctgctgggg gaggaagaca ttccccggga accaaggcgg
     1921 atcgtgatcc atcggggctc caccggcctg ggcttcaaca tcgtgggcgg cgaggatggt
     1981 gaaggcatct tcatctcctt catccttgct gggggtccag ccgacctcag tggggagcta
     2041 cggaaggggg accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcacgaa
     2101 caggctgcca ttgccctgaa gaatgcgggt cagacggtca cgatcatcgc tcagtataaa
     2161 ccagaagagt atagtcgatt cgaggccaag atccatgatc ttcgggaaca gctcatgaat
     2221 agtagcctag gctcagggac tgcatccttg cgaagcaacc ccaagagggg cttctacatt
     2281 agggccctgt ttgattacga caagaccaag gactgcggtt tcttgagcca ggccctgagc
     2341 ttccgcttcg gggatgtgct tcatgtcatt gacgctggtg acgaagagtg gtggcaagca
     2401 cggcgggtcc actccgacag tgagaccgac gacattggct tcattcccag caaacggcgg
     2461 gtcgagcgac gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca
     2521 cagggtcgag aagactcggt tctgagctat gagacggtga cccagatgga agtgcactat
     2581 gctcgtccca tcatcatcct tggacccacc aaagaccgtg ccaacgatga tcttctctcc
     2641 gagttccccg acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat
     2701 gagatagacg gccgggatta ccactttgtc tcctcccggg agaaaatgga gaaggacatc
     2761 caggcacaca agttcattga ggctggccag tacaacagcc acctctatgg gaccagcgtc
     2821 cagtctgtgc gagaggtagc agagcagggg aagcactgca tcctcgatgt ctcggccaat
     2881 gccgtgcggc ggctgcaggc ggcccacctg caccccatcg ccatcttcat ccgtccccgc
     2941 tccctggaga atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc
     3001 ttcgacagag ccacgaagct ggagcaggag ttcacagagt gcttctcagc catcgtagag
     3061 ggcgacagct ttgaagagat ctatcacaaa gtgaaacgtg tcattgaaga cctctcaggc
     3121 ccctacatct gggtcccagc ccgagagaga ctggtaccgc gggcccggga tccaccggtc
     3181 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3241 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3301 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3361 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3421 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3481 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3541 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3601 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3661 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3721 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3781 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     3841 atggtcctgc tggagttcgt gaccgccgcc cgcatgcata agttttctca agaacagatc
     3901 ggcgaaaaca ttgtgtgcag ggtcatttgt accacgggtc aaattcccat ccgagatttg
     3961 tcagctgata tttcacaagt gcttaaggaa aaacgatcca taaagaaagt ttggacattt
     4021 ggtagaaacc cagcctgtga ctatcattta ggaaacattt caagactgtc aaataagcat
     4081 ttccaaatac tactaggaga agacggtaac cttttattga atgacatttc cactaatggg
     4141 acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc tcaaggtgat
     4201 gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat tttcataaac
     4261 gacaaattta agcagtgcct cgagcagaac aaagttgatc gctctgcagg taagccaggc
     4321 agcggcgagg gcagcaccaa gggcctgcgt cgcgccaccc tggtggacgg cggcaccggc
     4381 ggcagcgagc tcatgggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     4501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agagagtgac cgccgccggg
     4561 atcactctcg gcatggacga gctatacaag ggtggaactg gaggttcaat ggtgagcaag
     4621 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4681 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4741 ctgaagctca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4801 ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     4861 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4921 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4981 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5041 aactggaaca gcgtcaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5101 aacttcaaga tccgccacaa catcgagtaa gcggccgcaa ttccccgggg atcctctaga
     5161 gtcgacctgc agaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt
     5221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat cgggaattaa
     5341 ttcggcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     5401 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5461 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5581 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5641 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5701 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5761 ctgttaacag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5821 ttacccaact taatcgcctt gcagcacatc cccccttcgc cagctggcgt aatagcgaag
     5881 aggcccgcac cgatcgccct tcccaacagt tgcgtagcct gaatggcgaa tggcgcctga
     5941 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     6001 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     6241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     6361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6421 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     6481 aatctgctct gatgccgcat agttaagcca actccgctat cgctacgtga ctgggtcatg
     6541 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6601 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6661 ccgtcatcac cgaaacgcgc gaggcagtat tcttgaagac gaaagggcct cgtgatacgc
     6721 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7141 gatgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcca
     7441 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8041 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8281 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     8341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc cagctggcac
     8761 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc
     8821 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8881 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attaa
//
