LOCUS       Exported                7181 bp ds-DNA     circular UNA 01-NOV-2018
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...c-6p7vp5i
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..3451
                     /label=AGCTCCAGGCGATCTGACGGTTCACTAAACGAGTTCTGCTTATATAG
                     /label=AGCTCCAGGCGATCTGACGGTTCACTAAACGAGTTCTGCTTATATAGGCC 
                     TCCCACCGTACACGCCTACCCCCCGTACCCCATTGACGTCAATGGGGGATCTCGAGTA 
                     CCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGACGTCAATGGGGGATCTGG 
                     AGTACCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGACGTCAATGGGGGAT 
                     CTGGAGTACCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGACGTCAATGGG 
                     GGATCTGGAGTACCCCATTGACGTCAATGCATGGGGGTAGGCGTGTACGGTGGGAGGC 
                     CTATATAAGCAGAACTCGTTTAGTGAACCGTCAGATCGCCTGGAGCTCGCCACCATGG 
                     TGAGCAAGGGCGAGGAGGTCATCAAAGAGTTCATGCGCTTCAAGGTGCGCATGGAGGG 
                     CTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAG 
                     GGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGG 
                     ACATCCTGTCCCCCCAGTTCATGTACGGCTCCAAGGCGTACGTGAAGCACCCCGCCGA 
                     CATCCCCGATTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATG 
                     AACTTCGAGGACGGCGGTCTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCA 
                     CGCTGATCTACAAGGTGAAGATGCGCGGCACCAACTTCCCCCCCGACGGCCCCGTAAT 
                     GCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGC 
                     GTGCTGAAGGGCGAGATCCACCAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGG 
                     TGGAGTTCAAGACCATCTACATGGCCAAGAAGCCCGTGCAACTGCCCGGCTACTACTA 
                     CGTGGACACCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAG 
                     TACGAGCGCTCCGAGGGCCGCCACCACCTGTTCCTGGGGCATGGCACCGGCAGCACCG 
                     GCAGCGGCAGCTCCGGCACCGCCTCCTCCGAGGACAACAACATGGCCGTCATCAAAGA 
                     GTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATC 
                     GAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGA 
                     CCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCATGTACGG 
                     CTCCAAGGCGTACGTGAAGCACCCCGCCGACATCCCCGATTACAAGAAGCTGTCCTTC 
                     CCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGTCTGGTGACCG 
                     TGACCCAGGACTCCTCCCTGCAGGACGGCACGCTGATCTACAAGGTGAAGATGCGCGG 
                     CACCAACTTCCCCCCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC 
                     TCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGATCCACCAGGCCC 
                     TGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGACCATCTACATGGCCAA 
                     GAAGCCCGTGCAACTGCCCGGCTACTACTACGTGGACACCAAGCTGGACATCACCTCC 
                     CACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCTCCGAGGGCCGCCACCACC 
                     TGTTCCTGTACGGCATGGACGAGCTGTACAAGTAAATGCA
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=AGCTCCAGGCGATCTGACGGTTCACTAAACGAGTTC
                     TGCTT 
                     ATATAGGCCTCCCACCGTACACGCCTACCCCCCGTACCCCATTGACGTCAATGGGGGA 
                     TCTCGAGTACCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGACGTCAATGG 
                     GGGATCTGGAGTACCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGACGTCA 
                     ATGGGGGATCTGGAGTACCCCATTGACGTCAATGGGGGATCTCGAGTACCCCATTGAC 
                     GTCAATGGGGGATCTGGAGTACCCCATTGACGTCAATGCATGGGGGTAGGCGTGTACG 
                     GTGGGAGGCCTATATAAGCAGAACTCGTTTAGTGAACCGTCAGATCGCCTGGAGCTCG 
                     CCACCATGGTGAGCAAGGGCGAGGAGGTCATCAAAGAGTTCATGCGCTTCAAGGTGCG 
                     CATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGC 
                     CCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCT 
                     TCGCCTGGGACATCCTGTCCCCCCAGTTCATGTACGGCTCCAAGGCGTACGTGAAGCA 
                     CCCCGCCGACATCCCCGATTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAG 
                     CGCGTGATGAACTTCGAGGACGGCGGTCTGGTGACCGTGACCCAGGACTCCTCCCTGC 
                     AGGACGGCACGCTGATCTACAAGGTGAAGATGCGCGGCACCAACTTCCCCCCCGACGG 
                     CCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCC 
                     CGCGACGGCGTGCTGAAGGGCGAGATCCACCAGGCCCTGAAGCTGAAGGACGGCGGCC 
                     ACTACCTGGTGGAGTTCAAGACCATCTACATGGCCAAGAAGCCCGTGCAACTGCCCGG 
                     CTACTACTACGTGGACACCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATC 
                     GTGGAACAGTACGAGCGCTCCGAGGGCCGCCACCACCTGTTCCTGGGGCATGGCACCG 
                     GCAGCACCGGCAGCGGCAGCTCCGGCACCGCCTCCTCCGAGGACAACAACATGGCCGT 
                     CATCAAAGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCATGAACGGCCACGAG 
                     TTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGC 
                     TGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTT 
                     CATGTACGGCTCCAAGGCGTACGTGAAGCACCCCGCCGACATCCCCGATTACAAGAAG 
                     CTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGTC 
                     TGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCACGCTGATCTACAAGGTGAA 
                     GATGCGCGGCACCAACTTCCCCCCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGC 
                     TGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGATCC 
                     ACCAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGACCATCTA 
                     CATGGCCAAGAAGCCCGTGCAACTGCCCGGCTACTACTACGTGGACACCAAGCTGGAC 
                     ATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCTCCGAGGGCC 
                     GCCACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGTAAATGCA"
     misc_feature    3..6
                     /label=Ligation
                     /label=nonstandard type: ligation
     enhancer        15..372
                     /label=CMV IE Enhancer
     misc_feature    373..393
                     /label=NRSE
     misc_feature    395..398
                     /label=Ligation
                     /label=nonstandard type: ligation
     misc_feature    400..871
                     /label=synapsin promoter
                     /note="synapsin promoter"
     CDS             885..2825
                     /label=AKAR5.1 open ended (EGFPdelta/cp-sREACh version)
     misc_recomb     885..1568
                     /label=mEGFPdelta
     CDS             2847..3443
                     /label=MTBD (MAP2c microtubule binding domain)
     misc_feature    3465..4059
                     /label=WRE
     polyA_signal    4067..4296
                     /label=SV40pA
     misc_feature    4297..4300
                     /label=Ligation
                     /label=nonstandard type: ligation
     repeat_region   4303..4443
                     /label=Repeat_Region_2
                     /note="/Description=right AAV-2ITR"
     rep_origin      4518..4978
                     /label=Rep_Origin_1
                     /note="/Description=f1"
     CDS             5360..6220
                     /product="""""beta-lactamase"
                     /label=CDS_1
                     /note="/Description=ampicillin resistance"
                     /db_xref="""""GI:15148787"
                     /protein_id="""""AAK84839.1"
     rep_origin      6232..7040
                     /label=Rep_Origin_2
                     /note="/Description=ColE1"
     repeat_region   7041..7181
                     /label=Repeat_Region_1
                     /note="/Description=left AAV-2ITR"
     misc_feature    7181
                     /label=GC
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=GC"
ORIGIN
        1 gcggccgcgg tacctattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac cattcagaac cacggacagc accgaattct tgcagagggc cctgcgtatg
      421 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      481 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      541 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      601 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      661 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      721 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      781 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      841 aggagtcgtg tcgtgcctga gagcgcagtc ggccggtcgc caccatggtg agcaagggcg
      901 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      961 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1021 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1081 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1141 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1201 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1261 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1321 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1381 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1441 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1501 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1561 ccgccgcccg catgcataag ttttctcaag aacagatcgg cgaaaacatt gtgtgcaggg
     1621 tcatttgtac cacgggtcaa attcccatcc gagatttgtc agctgatatt tcacaagtgc
     1681 ttaaggaaaa acgatccata aagaaagttt ggacatttgg tagaaaccca gcctgtgact
     1741 atcatttagg aaacatttca agactgtcaa ataagcattt ccaaatacta ctaggagaag
     1801 acggtaacct tttattgaat gacatttcca ctaatgggac ctggttaaat gggcaaaaag
     1861 tcgagaagaa cagcaatcag ttactgtctc aaggtgatga aataaccgtt ggtgtaggcg
     1921 tggaatcaga tattttatct ctggtcattt tcataaacga caaatttaag cagtgcctcg
     1981 agcagaacaa agttgatcgc tctgcaggta agccaggcag cggcgagggc agcaccaagg
     2041 gcctgcgtcg cgccaccctg gtggacggcg gcaccggcgg cagcgagctc atgggcagcg
     2101 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2161 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2221 atcacatggt cctgctggag agagtgaccg ccgccgggat cactctcggc atggacgagc
     2281 tatacaaggg tggaactgga ggttcaatgg tgagcaaggg cgaggagctg ttcaccgggg
     2341 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2401 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctcatc tgcaccaccg
     2461 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
     2521 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2581 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2641 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2701 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctggaacagc gtcaacgtct
     2761 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2821 tcgagtccgg agtgtacaag ggtaccccag gaacccccaa gtctgccatc ttggtgccga
     2881 gtgagaagaa ggtcgccatc atacgtactc ctccaaaatc tcctgcgact cccaagcagc
     2941 ttcggcttat taaccaacca ctgccagacc tgaagaatgt caaatccaaa atcggatcaa
     3001 cagacaacat caaataccag cctaaagggg ggcaggtaca aattgttacc aagaaaatag
     3061 acctaagcca tgtgacatcc aaatgtggct ctctgaagaa catccgccac aggccaggtg
     3121 gcggacgtgt gaaaattgag agtgtaaaac tagatttcaa agaaaaggcc caagctaaag
     3181 ttggttctct tgataatgct catcatgtac ctggaggtgg taatgtcaag attgacagcc
     3241 aaaagttgaa cttcagagag catgctaaag cccgtgtgga ccatggggct gagatcatta
     3301 cacagtcccc aggcagatcc agcgtggcat caccccgacg actcagcaat gtctcctcgt
     3361 ctggaagcat caacctgctc gaatctcctc agcttgccac tttggctgag gatgtcactg
     3421 ctgcactcgc taagcagggc ttgtaatcta ggaacggccg ttatcgataa tcaacctctg
     3481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3541 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3601 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3661 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3721 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3781 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3841 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3901 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3961 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4021 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gattgcggcc gtcagacatg
     4081 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4141 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4201 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     4261 ttttaaagca agtaaaacct ctacaaatgt ggtagcggcc gcaggaaccc ctagtgatgg
     4321 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4381 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4441 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4501 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4981 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5041 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5101 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5161 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5221 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5341 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5401 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5461 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5521 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5581 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5641 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5701 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5761 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5821 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5881 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5941 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6001 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6061 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6121 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6181 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6241 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6301 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6661 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6721 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6781 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6841 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6901 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6961 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7021 ctggcctttt gctcacatgt cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc
     7081 ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     7141 gcgcagagag ggagtggcca actccatcac taggggttcc t
//
