LOCUS       pMaCTag_16              3596 bp ds-DNA     circular     12-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1233..1252)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     join(3580..3596,1..1)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1492..2174)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     873..906
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1163..1196
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2272..2931)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..735
                     /locus_tag="Dronpa3"
                     /label="Dronpa3"
                     /ApEinfo_label="Dronpa3"
                     /ApEinfo_fwdcolor="#2dff00"
                     /ApEinfo_revcolor="#2dff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      745..866
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="#005ebb"
                     /ApEinfo_revcolor="#005ebb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        913..1161
                     /locus_tag="hU6 promoter"
                     /label="hU6 promoter"
                     /ApEinfo_label="hU6 promoter"
                     /ApEinfo_fwdcolor="#ff2fff"
                     /ApEinfo_revcolor="#ff2fff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    31..63
                     /locus_tag="GS-linker"
                     /label="GS-linker"
                     /ApEinfo_label="GS-linker"
                     /ApEinfo_fwdcolor="#8d8d8d"
                     /ApEinfo_revcolor="#8d8d8d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     31..49
                     /locus_tag="M1-primer binding-site"
                     /label="M1-primer binding-site"
                     /ApEinfo_label="M1-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1197..1214)
                     /locus_tag="M2-primer binding-site"
                     /label="M2-primer binding-site"
                     /ApEinfo_label="M2-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA
       61 TCCgtgagtg tgattaaacc agacatgaag atcaagctgc gtatggaagg cgctgtaaat
      121 ggacacccgt tcgcgattga aggagttggc cttgggaagc ctttcgaggg aaaacagagt
      181 atggacctta aagtcaaaga aggcggacct ctgcctttcg cctatgacat cttgacaact
      241 gtgttctgtt acggcaacag ggtattcgcc aaatacccag aaaatatagt agactatttc
      301 aagcagtcgt ttcctgaggg ctactcttgg gaacgaagca tgaattacga agacgggggc
      361 atttgtaacg cgacaaacga cataaccctg gatggtgact gttatatcta tgaaattcga
      421 tttgatggtg tgaactttcc tgccaatggt ccagttatgc agaagaggac tgtgaaatgg
      481 gagccatcca ctgagaaatt gtatgtgcgt gatggagtgc tgaagggtga tattaacgcg
      541 gctctgtcgc ttgaaggagg tggccattac cgatgtgact tcaaaactac ttataaagct
      601 aagaaggttg tccagttgcc agactatcac tttgtggacc accacattga gattaaaagc
      661 cacgacaaag attacagtaa tgttaatctg catgagcacg ccgaagcgca ttctgagctg
      721 ccgaggcagg ccaagTGAAC TAGTaacttg tttattgcag cttataatgg ttacaaataa
      781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      841 ttgtccaaac tcatcaatgt atcttaGTCG ATATAACTTC GTATAGCATA CATTATACGA
      901 AGTTATGTCG AGgagggcct atttcccatg attccttcat atttgcatat acgatacaag
      961 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     1021 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     1081 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     1141 ttgtggaaag gacgaaacac cGATAACTTC GTATAGCATA CATTATACGA AGTTATGGTA
     1201 CCGATGCAGC TAGCCCGCGG ATCTGCCGGT CTCCCTATAG TGAGTCGTAT TAATTTCGAT
     1261 AAGCCAGGTT AACCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
     1321 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
     1381 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     1441 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     1501 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1561 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1621 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1681 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1741 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1801 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1861 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1921 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
     1981 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2041 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2101 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2161 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     2221 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     2281 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     2341 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2401 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2461 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2521 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2581 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2641 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2701 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2761 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2821 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2881 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2941 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     3001 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     3061 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     3121 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     3181 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT
     3241 TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA
     3301 AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT
     3361 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG
     3421 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC
     3481 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGGAC ATATTGTCGT TAGAACGCGG
     3541 CTACAATTAA TACATAACCT TATGTATCAT ACACATACGA TTTAGGTGAC ACTATA
//
