LOCUS pMaCTag_34 2990 bp ds-DNA circular 12-NOV-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(627..646) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind join(2974..2990,1..1) /locus_tag="SP6" /label="SP6" /ApEinfo_label="SP6" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(886..1568) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 267..300 /locus_tag="LoxP" /label="LoxP" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 557..590 /locus_tag="LoxP(1)" /label="LoxP(1)" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(1666..2325) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 106..129 /locus_tag="FLAG" /label="FLAG" /ApEinfo_label="FLAG" /ApEinfo_fwdcolor="#d4a18c" /ApEinfo_revcolor="#d4a18c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 139..260 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#005ebb" /ApEinfo_revcolor="#005ebb" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 307..555 /locus_tag="hU6 promoter" /label="hU6 promoter" /ApEinfo_label="hU6 promoter" /ApEinfo_fwdcolor="#ff2fff" /ApEinfo_revcolor="#ff2fff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 31..63 /locus_tag="GS-linker" /label="GS-linker" /ApEinfo_label="GS-linker" /ApEinfo_fwdcolor="#8d8d8d" /ApEinfo_revcolor="#8d8d8d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 31..49 /locus_tag="M1-primer binding-site" /label="M1-primer binding-site" /ApEinfo_label="M1-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(591..608) /locus_tag="M2-primer binding-site" /label="M2-primer binding-site" /ApEinfo_label="M2-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 64..129 /locus_tag="3xFLAG" /label="3xFLAG" /ApEinfo_label="3xFLAG" /ApEinfo_fwdcolor="#b16363" /ApEinfo_revcolor="#b16363" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA 61 TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ATATCGATTA CAAGGATGAC 121 GATGACAAGT GAACTAGTaa cttgtttatt gcagcttata atggttacaa ataaagcaat 181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 241 aaactcatca atgtatctta GTCGATATAA CTTCGTATAG CATACATTAT ACGAAGTTAT 301 GTCGAGgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt 361 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga 421 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac 481 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg 541 aaaggacgaa acaccGATAA CTTCGTATAG CATACATTAT ACGAAGTTAT GGTACCGATG 601 CAGCTAGCCC GCGGATCTGC CGGTCTCCCT ATAGTGAGTC GTATTAATTT CGATAAGCCA 661 GGTTAACCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 721 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 781 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 841 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 901 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 961 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1021 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1081 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1141 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1201 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1261 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1321 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1381 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1441 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1501 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1561 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 1621 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 1681 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 1741 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 1801 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 1861 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 1921 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 1981 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2041 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2101 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2161 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2221 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2281 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2341 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2401 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2461 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2521 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 2581 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 2641 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 2701 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2761 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2821 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2881 AGAGCAGATT GTACTGAGAG TGCACCATAT GGACATATTG TCGTTAGAAC GCGGCTACAA 2941 TTAATACATA ACCTTATGTA TCATACACAT ACGATTTAGG TGACACTATA //