LOCUS pMaCTag_P06 4804 bp ds-DNA circular 12-NOV-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(2441..2460) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind join(4788..4804,1..1) /locus_tag="SP6" /label="SP6" /ApEinfo_label="SP6" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2700..3382) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 912..945 /locus_tag="LoxP" /label="LoxP" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 2371..2404 /locus_tag="LoxP(1)" /label="LoxP(1)" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(3480..4139) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 64..774 /locus_tag="sfGFP" /label="sfGFP" /ApEinfo_label="sfGFP" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 784..905 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#005ebb" /ApEinfo_revcolor="#005ebb" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 952..1268 /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1887..2114 /locus_tag="BGH polyA" /label="BGH polyA" /ApEinfo_label="BGH polyA" /ApEinfo_fwdcolor="#006ad5" /ApEinfo_revcolor="#006ad5" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2121..2369 /locus_tag="hU6 promoter" /label="hU6 promoter" /ApEinfo_label="hU6 promoter" /ApEinfo_fwdcolor="#ff2fff" /ApEinfo_revcolor="#ff2fff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 31..63 /locus_tag="GS-linker" /label="GS-linker" /ApEinfo_label="GS-linker" /ApEinfo_fwdcolor="#8d8d8d" /ApEinfo_revcolor="#8d8d8d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 31..49 /locus_tag="M1-primer binding-site" /label="M1-primer binding-site" /ApEinfo_label="M1-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2405..2422) /locus_tag="M2-primer binding-site" /label="M2-primer binding-site" /ApEinfo_label="M2-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 1281..1880 /locus_tag="PuromycinR" /label="PuromycinR" /ApEinfo_label="PuromycinR" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA 61 TCCAGCAAGG GCGAGGAGCT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA GCTGGACGGC 121 GACGTGAACG GCCACAAGTT CAGCGTGCGC GGCGAGGGCG AGGGCGACGC CACCAACGGC 181 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG 241 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCGC 301 CACGACTTCT TCAAGAGCGC CATGCCCGAG GGCTACGTGC AGGAGCGCAC CATCAGCTTC 361 AAGGACGACG GCACCTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 421 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGCCACAAG 481 CTGGAGTACA ACTTCAACAG CCACAACGTG TACATCACCG CCGACAAGCA GAAGAACGGC 541 ATCAAGGCCA ACTTCAAGAT CCGCCACAAC GTGGAGGACG GCAGCGTGCA GCTGGCCGAC 601 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 661 CTGAGCACCC AGAGCCGCCT GAGCAAGGAC CCCAACGAGA AGCGCGACCA CATGGTGCTG 721 CTGGAGTTCG TGACCGCCGC CGGCATCACC CACGGCATGG ACGAGCTGTA CAAGTGAACT 781 AGTaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 841 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 901 tcttaGTCGA TATAACTTCG TATAGCATAC ATTATACGAA GTTATGTCGA Cggtgtggaa 961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 1021 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 1081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 1141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 1201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 1261 tttgcaaaga attcGCCACC ATGACTGAGT ACAAGCCTAC TGTGCGTTTA GCCACCAGGG 1321 ATGATGTGCC ACGAGCCGTG AGGACACTAG CAGCCGCCTT TGCTGACTAC CCAGCTACAA 1381 GGCACACCGT GGACCCTGAC AGACACATTG AAAGAGTCAC AGAACTTCAG GAGCTGTTTT 1441 TAACTAGGGT GGGCCTGGAT ATTGGCAAAG TCTGGGTGGC CGATGATGGA GCAGCAGTTG 1501 CAGTGTGGAC CACCCCAGAG TCTGTTGAAG CTGGAGCTGT CTTTGCAGAA ATTGGACCAA 1561 GAATGGCCGA GTTATCTGGC AGCAGGCTGG CTGCTCAACA GCAGATGGAA GGATTATTAG 1621 CTCCCCACAG ACCCAAGGAG CCTGCCTGGT TCCTGGCAAC AGTTGGAGTT TCTCCAGACC 1681 ATCAGGGCAA GGGCTTAGGA TCTGCTGTGG TGCTGCCTGG TGTGGAAGCA GCTGAGAGAG 1741 CTGGGGTGCC AGCATTTCTG GAGACATCAG CTCCTAGAAA CCTGCCCTTC TATGAACGGC 1801 TGGGCTTCAC AGTGACTGCT GATGTGGAGG TGCCTGAGGG CCCCAGAACC TGGTGCATGA 1861 CAAGAAAACC AGGAGCCTGA CTGCAGcgac tgtgccttct agttgccagc catctgttgt 1921 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1981 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 2041 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 2101 ggtgggctct atggCTCGAG gagggcctat ttcccatgat tccttcatat ttgcatatac 2161 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag 2221 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 2281 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 2341 tatatatctt gtggaaagga cgaaacaccG ATAACTTCGT ATAGCATACA TTATACGAAG 2401 TTATGGTACC GATGCAGCTA GCCCGCGGAT CTGCCGGTCT CCCTATAGTG AGTCGTATTA 2461 ATTTCGATAA GCCAGGTTAA CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 2521 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 2581 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 2641 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 2701 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 2761 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 2821 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 2881 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG 2941 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 3001 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 3061 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 3121 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 3181 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 3241 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 3301 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 3361 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 3421 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 3481 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 3541 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 3601 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 3661 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 3721 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 3781 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 3841 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 3901 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 3961 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 4021 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 4081 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 4141 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 4201 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 4261 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 4321 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 4381 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 4441 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 4501 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 4561 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 4621 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 4681 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA 4741 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC 4801 TATA //