LOCUS       pMaCTag_P18             4756 bp ds-DNA     circular     12-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2393..2412)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     join(4740..4756,1..1)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2652..3334)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     864..897
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2323..2356
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3432..4091)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..726
                     /locus_tag="hmKeima8.5"
                     /label="hmKeima8.5"
                     /ApEinfo_label="hmKeima8.5"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      736..857
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="#005ebb"
                     /ApEinfo_revcolor="#005ebb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        904..1220
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1839..2066
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#006ad5"
                     /ApEinfo_revcolor="#006ad5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2073..2321
                     /locus_tag="hU6 promoter"
                     /label="hU6 promoter"
                     /ApEinfo_label="hU6 promoter"
                     /ApEinfo_fwdcolor="#ff2fff"
                     /ApEinfo_revcolor="#ff2fff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    31..63
                     /locus_tag="GS-linker"
                     /label="GS-linker"
                     /ApEinfo_label="GS-linker"
                     /ApEinfo_fwdcolor="#8d8d8d"
                     /ApEinfo_revcolor="#8d8d8d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     31..49
                     /locus_tag="M1-primer binding-site"
                     /label="M1-primer binding-site"
                     /ApEinfo_label="M1-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2357..2374)
                     /locus_tag="M2-primer binding-site"
                     /label="M2-primer binding-site"
                     /ApEinfo_label="M2-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1233..1832
                     /locus_tag="PuromycinR"
                     /label="PuromycinR"
                     /ApEinfo_label="PuromycinR"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA
       61 TCCGTGAGCG TGATCGCCGA GCACATGACC TACAAGGTGT ACATGAGCGG CACCGTGAAC
      121 GGCCACTACT TCGAGGTGGA GGGCGACGGC GAGGGCAAGC CCTACGAGGG CGAGCAGACC
      181 GTGAAGCTGA CCGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGCACAT CCTGAGCCCC
      241 CAGCTCCAGT ACGGCAGCAT CCCCTTCACC AAGTACCCCG CCgacatcCC CGACTACTTC
      301 AAGCAGAGCT TCCCCGAGGG CTACACCTGG GTGCGCAGCA TGAACTTCGA GGACGGCGCC
      361 GTGTGCACCG TGAGCAACGA CAGCAGCATC CGCGGCAACT GCTTCATCTA CAACGTGAAG
      421 ATCAGCGGCG AGAACTTCCC CCCCAACGGC CCCGTGATGC AGAAGAAGAC CCAGGGCTGG
      481 GAGCCCAGCA CCGAGCGCAT GTTCGCCCGC GACGGAATGC TGATCGGCAA CGACCACATG
      541 GCCCTGAAGC TGGAGGGCGG CGGCCACTAC CTGTGCGAGT TCAAGAGCGC CTACAAGGCC
      601 CGCAAGCCCG TGAGGATGCC CGGCCTGTAC GAGATCGACC GCAAGCTGGA CGTGACCAGC
      661 CACAACCGCG ACTACACCAG CGTGGAGCAG CGCGAGATCG CCATCGCCCG CCACCCCCTG
      721 CTGGGCTGAA CTAGTaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      841 ctcatcaatg tatcttaGTC GATATAACTT CGTATAGCAT ACATTATACG AAGTTATGTC
      901 GACggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      961 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     1021 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1201 gaggcctagg cttttgcaaa gaattcGCCA CCATGACTGA GTACAAGCCT ACTGTGCGTT
     1261 TAGCCACCAG GGATGATGTG CCACGAGCCG TGAGGACACT AGCAGCCGCC TTTGCTGACT
     1321 ACCCAGCTAC AAGGCACACC GTGGACCCTG ACAGACACAT TGAAAGAGTC ACAGAACTTC
     1381 AGGAGCTGTT TTTAACTAGG GTGGGCCTGG ATATTGGCAA AGTCTGGGTG GCCGATGATG
     1441 GAGCAGCAGT TGCAGTGTGG ACCACCCCAG AGTCTGTTGA AGCTGGAGCT GTCTTTGCAG
     1501 AAATTGGACC AAGAATGGCC GAGTTATCTG GCAGCAGGCT GGCTGCTCAA CAGCAGATGG
     1561 AAGGATTATT AGCTCCCCAC AGACCCAAGG AGCCTGCCTG GTTCCTGGCA ACAGTTGGAG
     1621 TTTCTCCAGA CCATCAGGGC AAGGGCTTAG GATCTGCTGT GGTGCTGCCT GGTGTGGAAG
     1681 CAGCTGAGAG AGCTGGGGTG CCAGCATTTC TGGAGACATC AGCTCCTAGA AACCTGCCCT
     1741 TCTATGAACG GCTGGGCTTC ACAGTGACTG CTGATGTGGA GGTGCCTGAG GGCCCCAGAA
     1801 CCTGGTGCAT GACAAGAAAA CCAGGAGCCT GACTGCAGcg actgtgcctt ctagttgcca
     1861 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1921 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1981 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2041 tgctggggat gcggtgggct ctatggCTCG AGgagggcct atttcccatg attccttcat
     2101 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     2161 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2221 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2281 atttcttggc tttatatatc ttgtggaaag gacgaaacac cGATAACTTC GTATAGCATA
     2341 CATTATACGA AGTTATGGTA CCGATGCAGC TAGCCCGCGG ATCTGCCGGT CTCCCTATAG
     2401 TGAGTCGTAT TAATTTCGAT AAGCCAGGTT AACCTGCATT AATGAATCGG CCAACGCGCG
     2461 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
     2521 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     2581 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     2641 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     2701 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     2761 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     2821 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG
     2881 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     2941 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     3001 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     3061 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
     3121 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     3181 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     3241 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     3301 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     3361 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3421 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     3481 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     3541 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
     3601 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3661 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3721 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3781 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3841 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3901 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3961 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     4021 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     4081 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     4141 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     4201 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     4261 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     4321 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     4381 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT
     4441 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC
     4501 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     4561 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     4621 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGGAC
     4681 ATATTGTCGT TAGAACGCGG CTACAATTAA TACATAACCT TATGTATCAT ACACATACGA
     4741 TTTAGGTGAC ACTATA    
//
