LOCUS pMaCTag_P28 4183 bp ds-DNA circular 12-NOV-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(1820..1839) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind join(4167..4183,1..1) /locus_tag="SP6" /label="SP6" /ApEinfo_label="SP6" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2079..2761) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 291..324 /locus_tag="LoxP" /label="LoxP" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 1750..1783 /locus_tag="LoxP(1)" /label="LoxP(1)" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(2859..3518) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 64..153 /locus_tag="3xHA" /label="3xHA" /ApEinfo_label="3xHA" /ApEinfo_fwdcolor="#9e6852" /ApEinfo_revcolor="#9e6852" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 163..284 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#005ebb" /ApEinfo_revcolor="#005ebb" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 331..647 /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1266..1493 /locus_tag="BGH polyA" /label="BGH polyA" /ApEinfo_label="BGH polyA" /ApEinfo_fwdcolor="#006ad5" /ApEinfo_revcolor="#006ad5" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1500..1748 /locus_tag="hU6 promoter" /label="hU6 promoter" /ApEinfo_label="hU6 promoter" /ApEinfo_fwdcolor="#ff2fff" /ApEinfo_revcolor="#ff2fff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 31..63 /locus_tag="GS-linker" /label="GS-linker" /ApEinfo_label="GS-linker" /ApEinfo_fwdcolor="#8d8d8d" /ApEinfo_revcolor="#8d8d8d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 31..49 /locus_tag="M1-primer binding-site" /label="M1-primer binding-site" /ApEinfo_label="M1-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1784..1801) /locus_tag="M2-primer binding-site" /label="M2-primer binding-site" /ApEinfo_label="M2-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 660..1259 /locus_tag="PuromycinR" /label="PuromycinR" /ApEinfo_label="PuromycinR" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA 61 TCCtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca 121 ggatcctatc catatgacgt tccagattac gctTGAACTA GTaacttgtt tattgcagct 181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaGTCGAT ATAACTTCGT 301 ATAGCATACA TTATACGAAG TTATGTCGAC ggtgtggaaa gtccccaggc tccccagcag 361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 481 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 541 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 601 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagaa ttcGCCACCA 661 TGACTGAGTA CAAGCCTACT GTGCGTTTAG CCACCAGGGA TGATGTGCCA CGAGCCGTGA 721 GGACACTAGC AGCCGCCTTT GCTGACTACC CAGCTACAAG GCACACCGTG GACCCTGACA 781 GACACATTGA AAGAGTCACA GAACTTCAGG AGCTGTTTTT AACTAGGGTG GGCCTGGATA 841 TTGGCAAAGT CTGGGTGGCC GATGATGGAG CAGCAGTTGC AGTGTGGACC ACCCCAGAGT 901 CTGTTGAAGC TGGAGCTGTC TTTGCAGAAA TTGGACCAAG AATGGCCGAG TTATCTGGCA 961 GCAGGCTGGC TGCTCAACAG CAGATGGAAG GATTATTAGC TCCCCACAGA CCCAAGGAGC 1021 CTGCCTGGTT CCTGGCAACA GTTGGAGTTT CTCCAGACCA TCAGGGCAAG GGCTTAGGAT 1081 CTGCTGTGGT GCTGCCTGGT GTGGAAGCAG CTGAGAGAGC TGGGGTGCCA GCATTTCTGG 1141 AGACATCAGC TCCTAGAAAC CTGCCCTTCT ATGAACGGCT GGGCTTCACA GTGACTGCTG 1201 ATGTGGAGGT GCCTGAGGGC CCCAGAACCT GGTGCATGAC AAGAAAACCA GGAGCCTGAC 1261 TGCAGcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1321 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1381 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 1441 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggCTCGAGg 1501 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga 1561 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa 1621 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata 1681 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac 1741 gaaacaccGA TAACTTCGTA TAGCATACAT TATACGAAGT TATGGTACCG ATGCAGCTAG 1801 CCCGCGGATC TGCCGGTCTC CCTATAGTGA GTCGTATTAA TTTCGATAAG CCAGGTTAAC 1861 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC 1921 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 1981 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 2041 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 2101 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 2161 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 2221 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 2281 GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 2341 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 2401 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 2461 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 2521 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 2581 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 2641 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 2701 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 2761 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 2821 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 2881 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 2941 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC 3001 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 3061 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 3121 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 3181 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 3241 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 3301 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 3361 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 3421 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 3481 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 3541 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 3601 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 3661 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 3721 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 3781 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 3841 CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC 3901 ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG 3961 CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG 4021 GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG 4081 ATTGTACTGA GAGTGCACCA TATGGACATA TTGTCGTTAG AACGCGGCTA CAATTAATAC 4141 ATAACCTTAT GTATCATACA CATACGATTT AGGTGACACT ATA //