LOCUS       pMaCTag_P30             4126 bp ds-DNA     circular     12-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1763..1782)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     join(4110..4126,1..1)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2022..2704)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     234..267
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1693..1726
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2802..3461)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..96
                     /locus_tag="VSVG"
                     /label="VSVG"
                     /ApEinfo_label="VSVG"
                     /ApEinfo_fwdcolor="#ba8f72"
                     /ApEinfo_revcolor="#ba8f72"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      106..227
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="#005ebb"
                     /ApEinfo_revcolor="#005ebb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        274..590
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1209..1436
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#006ad5"
                     /ApEinfo_revcolor="#006ad5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1443..1691
                     /locus_tag="hU6 promoter"
                     /label="hU6 promoter"
                     /ApEinfo_label="hU6 promoter"
                     /ApEinfo_fwdcolor="#ff2fff"
                     /ApEinfo_revcolor="#ff2fff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    31..63
                     /locus_tag="GS-linker"
                     /label="GS-linker"
                     /ApEinfo_label="GS-linker"
                     /ApEinfo_fwdcolor="#8d8d8d"
                     /ApEinfo_revcolor="#8d8d8d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     31..49
                     /locus_tag="M1-primer binding-site"
                     /label="M1-primer binding-site"
                     /ApEinfo_label="M1-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1727..1744)
                     /locus_tag="M2-primer binding-site"
                     /label="M2-primer binding-site"
                     /ApEinfo_label="M2-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            603..1202
                     /locus_tag="PuromycinR"
                     /label="PuromycinR"
                     /ApEinfo_label="PuromycinR"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA
       61 TCCTATACAG ACATAGAGAT GAACCGACTT GGAAAGTGAA CTAGTaactt gtttattgca
      121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaGTC GATATAACTT
      241 CGTATAGCAT ACATTATACG AAGTTATGTC GACggtgtgg aaagtcccca ggctccccag
      301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gaattcGCCA
      601 CCATGACTGA GTACAAGCCT ACTGTGCGTT TAGCCACCAG GGATGATGTG CCACGAGCCG
      661 TGAGGACACT AGCAGCCGCC TTTGCTGACT ACCCAGCTAC AAGGCACACC GTGGACCCTG
      721 ACAGACACAT TGAAAGAGTC ACAGAACTTC AGGAGCTGTT TTTAACTAGG GTGGGCCTGG
      781 ATATTGGCAA AGTCTGGGTG GCCGATGATG GAGCAGCAGT TGCAGTGTGG ACCACCCCAG
      841 AGTCTGTTGA AGCTGGAGCT GTCTTTGCAG AAATTGGACC AAGAATGGCC GAGTTATCTG
      901 GCAGCAGGCT GGCTGCTCAA CAGCAGATGG AAGGATTATT AGCTCCCCAC AGACCCAAGG
      961 AGCCTGCCTG GTTCCTGGCA ACAGTTGGAG TTTCTCCAGA CCATCAGGGC AAGGGCTTAG
     1021 GATCTGCTGT GGTGCTGCCT GGTGTGGAAG CAGCTGAGAG AGCTGGGGTG CCAGCATTTC
     1081 TGGAGACATC AGCTCCTAGA AACCTGCCCT TCTATGAACG GCTGGGCTTC ACAGTGACTG
     1141 CTGATGTGGA GGTGCCTGAG GGCCCCAGAA CCTGGTGCAT GACAAGAAAA CCAGGAGCCT
     1201 GACTGCAGcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     1261 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     1321 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     1381 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggCTCG
     1441 AGgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     1501 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     1561 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     1621 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1681 gacgaaacac cGATAACTTC GTATAGCATA CATTATACGA AGTTATGGTA CCGATGCAGC
     1741 TAGCCCGCGG ATCTGCCGGT CTCCCTATAG TGAGTCGTAT TAATTTCGAT AAGCCAGGTT
     1801 AACCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT
     1861 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
     1921 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
     1981 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     2041 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     2101 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     2161 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     2221 GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     2281 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     2341 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     2401 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     2461 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     2521 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     2581 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     2641 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2701 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2761 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2821 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2881 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2941 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     3001 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     3061 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     3121 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     3181 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3241 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3301 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3361 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3421 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3481 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3541 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3601 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3661 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3721 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
     3781 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
     3841 ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG
     3901 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT
     3961 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG
     4021 CAGATTGTAC TGAGAGTGCA CCATATGGAC ATATTGTCGT TAGAACGCGG CTACAATTAA
     4081 TACATAACCT TATGTATCAT ACACATACGA TTTAGGTGAC ACTATA 
//
