LOCUS pMaCTag_Z05 4582 bp ds-DNA circular 12-NOV-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(2219..2238) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind join(4566..4582,1..1) /locus_tag="SP6" /label="SP6" /ApEinfo_label="SP6" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2478..3160) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 915..948 /locus_tag="LoxP" /label="LoxP" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 2149..2182 /locus_tag="LoxP(1)" /label="LoxP(1)" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(3258..3917) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 64..777 /locus_tag="eGFP" /label="eGFP" /ApEinfo_label="eGFP" /ApEinfo_fwdcolor="#00ff38" /ApEinfo_revcolor="#00ff38" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 787..908 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#005ebb" /ApEinfo_revcolor="#005ebb" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 955..1271 /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 1284..1658 /locus_tag="BleoR" /label="BleoR" /ApEinfo_label="BleoR" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1665..1892 /locus_tag="BGH polyA" /label="BGH polyA" /ApEinfo_label="BGH polyA" /ApEinfo_fwdcolor="#006ad5" /ApEinfo_revcolor="#006ad5" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1899..2147 /locus_tag="hU6 promoter" /label="hU6 promoter" /ApEinfo_label="hU6 promoter" /ApEinfo_fwdcolor="#ff2fff" /ApEinfo_revcolor="#ff2fff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 31..63 /locus_tag="GS-linker" /label="GS-linker" /ApEinfo_label="GS-linker" /ApEinfo_fwdcolor="#8d8d8d" /ApEinfo_revcolor="#8d8d8d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 31..49 /locus_tag="M1-primer binding-site" /label="M1-primer binding-site" /ApEinfo_label="M1-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2183..2200) /locus_tag="M2-primer binding-site" /label="M2-primer binding-site" /ApEinfo_label="M2-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA 61 TCCgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 121 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 241 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 481 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 541 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 661 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 721 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagTAA 781 ACTAGTaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 901 gtatcttaGT CGATATAACT TCGTATAGCA TACATTATAC GAAGTTATGT CGACggtgtg 961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 1021 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 1081 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 1141 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 1201 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 1261 gcttttgcaa agaattcGCC ACCatggcca agttgaccag tgccgttccg gtgctcaccg 1321 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 1381 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 1441 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 1501 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 1561 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 1621 gcaactgcgt gcacttcgtg gccgaggagc aggactgaCT GCAGcgactg tgccttctag 1681 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 1741 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 1801 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 1861 caggcatgct ggggatgcgg tgggctctat ggCTCGAGga gggcctattt cccatgattc 1921 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg 1981 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 2041 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt 2101 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccGAT AACTTCGTAT 2161 AGCATACATT ATACGAAGTT ATGGTACCGA TGCAGCTAGC CCGCGGATCT GCCGGTCTCC 2221 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG AATCGGCCAA 2281 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 2341 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 2401 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 2461 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 2521 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 2581 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 2641 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC 2701 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 2761 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 2821 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 2881 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 2941 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 3001 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 3061 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 3121 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 3181 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 3241 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 3301 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 3361 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT 3421 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 3481 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 3541 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 3601 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 3661 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 3721 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 3781 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3841 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3901 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 3961 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 4021 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 4081 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 4141 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 4201 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC 4261 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG 4321 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 4381 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 4441 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 4501 ATGGACATAT TGTCGTTAGA ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC 4561 ATACGATTTA GGTGACACTA TA //