LOCUS       pMaCTag_Z21             5581 bp ds-DNA     circular     12-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(3218..3237)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     join(5565..5581,1..1)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3477..4159)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1914..1947
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     3148..3181
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4257..4916)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..1776
                     /locus_tag="24x SunTag"
                     /label="24x SunTag"
                     /ApEinfo_label="24x SunTag"
                     /ApEinfo_fwdcolor="#d3b8a5"
                     /ApEinfo_revcolor="#d3b8a5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..768
                     /locus_tag="10x Suntag"
                     /label="10x Suntag"
                     /ApEinfo_label="10x Suntag"
                     /ApEinfo_fwdcolor="#a35a3d"
                     /ApEinfo_revcolor="#a35a3d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1786..1907
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="#005ebb"
                     /ApEinfo_revcolor="#005ebb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1954..2270
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            2283..2657
                     /locus_tag="BleoR"
                     /label="BleoR"
                     /ApEinfo_label="BleoR"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2664..2891
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#006ad5"
                     /ApEinfo_revcolor="#006ad5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2898..3146
                     /locus_tag="hU6 promoter"
                     /label="hU6 promoter"
                     /ApEinfo_label="hU6 promoter"
                     /ApEinfo_fwdcolor="#ff2fff"
                     /ApEinfo_revcolor="#ff2fff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    31..63
                     /locus_tag="GS-linker"
                     /label="GS-linker"
                     /ApEinfo_label="GS-linker"
                     /ApEinfo_fwdcolor="#8d8d8d"
                     /ApEinfo_revcolor="#8d8d8d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     31..49
                     /locus_tag="M1-primer binding-site"
                     /label="M1-primer binding-site"
                     /ApEinfo_label="M1-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3182..3199)
                     /locus_tag="M2-primer binding-site"
                     /label="M2-primer binding-site"
                     /ApEinfo_label="M2-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA
       61 TCCgaagaac ttttgagcaa gaattatcat cttgagaacg aagtggctcg tcttaagaaa
      121 ggttctggca gtggagaaga actgctttca aagaattacc acctggaaaa tgaggtagct
      181 agactgaaaa aggggagcgg aagtggggag gagttgctga gcaaaaatta tcatttggag
      241 aacgaagtag cacgactaaa gaaagggtcc ggatcgggtg aggagttact ctcgaaaaat
      301 tatcatctcg aaaacgaagt ggctcggcta aaaaagggca gtggttctgg agaagagcta
      361 ttatctaaaa actaccacct cgaaaatgag gtggcacgct taaaaaaggg aagtggcagt
      421 ggtgaagagc tactatccaa gaattatcat cttgagaacg aggtagcgcg tttgaagaag
      481 ggttccggct caggagagga actgctctcg aagaactatc atcttgaaaa tgaggtcgct
      541 cgattaaaaa agggatcggg cagtggtgag gaactacttt caaagaatta ccacctcgaa
      601 aacgaagtag ctcgattaaa gaaaggttca gggtcgggtg aagaattact gagtaaaaat
      661 tatcatctgg aaaatgaggt agcgagacta aaaaagggga gtggttctgg cgaagagttg
      721 ctatcgaaaa attatcatct tgagaacgaa gttgctaggc tcaaaaaggg ctcaggctca
      781 ggcgaggagt tgctctcgaa aaactaccac ttggaaaatg aggtcgcgag gttgaaaaag
      841 gggagcgggt cgggcgagga gttattgagc aaaaactatc atttagagaa cgaagtcgcg
      901 cgcttaaaga aaggctcggg ctcgggcgaa gaactcttat cgaagaacta ccacctcgaa
      961 aatgaggtcg ccaggttgaa aaagggcagt ggcagcgggg aggaactctt gagcaagaac
     1021 taccacttgg agaatgaggt cgcgagattg aagaaagggt cggggagcgg cgaggaattg
     1081 ctcagcaaga attatcattt ggagaacgaa gtcgccaggc tcaagaaagg ctcggggtcg
     1141 ggggaggaat tgttgagtaa aaactaccac ttggaaaatg aagtcgccag gctcaaaaaa
     1201 gggagtggga gcggcgaaga gttattgagc aaaaattacc acttggagaa cgaagtggca
     1261 aggctcaaga aagggagcgg cagcggggag gagctcttat cgaagaacta ccacttagag
     1321 aatgaagtcg cccgcttgaa gaaaggctcg gggagcgggg aagagctctt gagcaagaac
     1381 taccacttgg aaaatgaggt ggcgcgcttg aagaaaggga gcgggagcgg ggaagagtta
     1441 ctatctaaga attatcatct cgagaacgag gtggctcgac taaagaaggg ctccggcagt
     1501 ggggaggaac tcctgtcgaa gaactatcat cttgaaaatg aggttgcaag acttaaaaag
     1561 gggtccggat caggtgagga actactcagt aagaattacc acctggaaaa cgaagttgca
     1621 cgtttgaaga aaggatcagg atcaggcgaa gaactgctct caaaagatta tcatttggaa
     1681 aatgaggttg cacgtttaaa aaagggaagt ggcagtggtg aggaacttct gtcgaaaaat
     1741 tatcatctcg agaatgaagt agcccgactt aaaaagTGAA CTAGTaactt gtttattgca
     1801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaGTC GATATAACTT
     1921 CGTATAGCAT ACATTATACG AAGTTATGTC GACggtgtgg aaagtcccca ggctccccag
     1981 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2041 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2101 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2161 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2221 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gaattcGCCA
     2281 CCatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     2341 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     2401 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     2461 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     2521 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     2581 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     2641 ccgaggagca ggactgaCTG CAGcgactgt gccttctagt tgccagccat ctgttgtttg
     2701 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2761 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     2821 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     2881 gggctctatg gCTCGAGgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     2941 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     3001 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     3061 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     3121 atatcttgtg gaaaggacga aacaccGATA ACTTCGTATA GCATACATTA TACGAAGTTA
     3181 TGGTACCGAT GCAGCTAGCC CGCGGATCTG CCGGTCTCCC TATAGTGAGT CGTATTAATT
     3241 TCGATAAGCC AGGTTAACCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     3301 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     3361 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     3421 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     3481 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     3541 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     3601 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     3661 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG
     3721 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     3781 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     3841 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     3901 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     3961 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     4021 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     4081 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     4141 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     4201 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     4261 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     4321 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     4381 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     4441 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     4501 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     4561 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     4621 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     4681 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     4741 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     4801 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     4861 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     4921 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     4981 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     5041 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     5101 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     5161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     5221 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
     5281 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA
     5341 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
     5401 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC
     5461 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGGACATATT GTCGTTAGAA
     5521 CGCGGCTACA ATTAATACAT AACCTTATGT ATCATACACA TACGATTTAG GTGACACTAT
     5581 A     
//
