LOCUS       pMaCTag_Z22             4411 bp ds-DNA     circular     12-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2048..2067)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     join(4395..4411,1..1)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2307..2989)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     744..777
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1978..2011
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3087..3746)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            64..606
                     /locus_tag="SNAP"
                     /label="SNAP"
                     /ApEinfo_label="SNAP"
                     /ApEinfo_fwdcolor="#b66444"
                     /ApEinfo_revcolor="#b66444"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      616..737
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="#005ebb"
                     /ApEinfo_revcolor="#005ebb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        784..1100
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1113..1487
                     /locus_tag="BleoR"
                     /label="BleoR"
                     /ApEinfo_label="BleoR"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1494..1721
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#006ad5"
                     /ApEinfo_revcolor="#006ad5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1728..1976
                     /locus_tag="hU6 promoter"
                     /label="hU6 promoter"
                     /ApEinfo_label="hU6 promoter"
                     /ApEinfo_fwdcolor="#ff2fff"
                     /ApEinfo_revcolor="#ff2fff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    31..63
                     /locus_tag="GS-linker"
                     /label="GS-linker"
                     /ApEinfo_label="GS-linker"
                     /ApEinfo_fwdcolor="#8d8d8d"
                     /ApEinfo_revcolor="#8d8d8d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     31..49
                     /locus_tag="M1-primer binding-site"
                     /label="M1-primer binding-site"
                     /ApEinfo_label="M1-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2012..2029)
                     /locus_tag="M2-primer binding-site"
                     /label="M2-primer binding-site"
                     /ApEinfo_label="M2-primer binding-site"
                     /ApEinfo_fwdcolor="#ff4f4f"
                     /ApEinfo_revcolor="#ff4f4f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA
       61 TCCgacaaag actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa
      121 ctgtctgggt gcgaacaggg cctgcacgag atcaagctgc tgggcaaagg aacatctgcc
      181 gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg
      241 caggccaccg cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct
      301 gtgccagccc tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg
      361 aaactgctga aagtggtgaa gttcggagag gtcatcagct accagcagct ggccgccctg
      421 gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc
      481 attctgatcc cctgccaccg ggtggtgtct agctctggcg ccgtgggggg ctacgagggc
      541 gggctcgccg tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg
      601 ctgggtTGAA CTAGTaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      721 ctcatcaatg tatcttaGTC GATATAACTT CGTATAGCAT ACATTATACG AAGTTATGTC
      781 GACggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      841 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      901 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1021 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1081 gaggcctagg cttttgcaaa gaattcGCCA CCatggccaa gttgaccagt gccgttccgg
     1141 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     1201 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     1261 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     1321 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     1381 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     1441 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaCTG CAGcgactgt
     1501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1621 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1681 agacaatagc aggcatgctg gggatgcggt gggctctatg gCTCGAGgag ggcctatttc
     1741 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     1801 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     1861 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     1921 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccGATA
     1981 ACTTCGTATA GCATACATTA TACGAAGTTA TGGTACCGAT GCAGCTAGCC CGCGGATCTG
     2041 CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC AGGTTAACCT GCATTAATGA
     2101 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     2161 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     2221 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     2281 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
     2341 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     2401 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     2461 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
     2521 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     2581 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     2641 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     2701 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     2761 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     2821 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     2881 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     2941 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     3001 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     3061 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     3121 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     3181 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
     3241 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     3301 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     3361 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3421 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3481 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3541 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3601 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3661 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3721 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3781 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3841 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3901 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3961 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     4021 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC
     4081 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT
     4141 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG
     4201 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG
     4261 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA
     4321 GTGCACCATA TGGACATATT GTCGTTAGAA CGCGGCTACA ATTAATACAT AACCTTATGT
     4381 ATCATACACA TACGATTTAG GTGACACTAT A  
//
