LOCUS pMaCTag_Z24 4411 bp ds-DNA circular 12-NOV-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(2048..2067) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind join(4395..4411,1..1) /locus_tag="SP6" /label="SP6" /ApEinfo_label="SP6" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2307..2989) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 744..777 /locus_tag="LoxP" /label="LoxP" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 1978..2011 /locus_tag="LoxP(1)" /label="LoxP(1)" /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(3087..3746) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 64..606 /locus_tag="TAP" /label="TAP" /ApEinfo_label="TAP" /ApEinfo_fwdcolor="#cd917a" /ApEinfo_revcolor="#cd917a" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 616..737 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#005ebb" /ApEinfo_revcolor="#005ebb" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 784..1100 /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 1113..1487 /locus_tag="BleoR" /label="BleoR" /ApEinfo_label="BleoR" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1494..1721 /locus_tag="BGH polyA" /label="BGH polyA" /ApEinfo_label="BGH polyA" /ApEinfo_fwdcolor="#006ad5" /ApEinfo_revcolor="#006ad5" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1728..1976 /locus_tag="hU6 promoter" /label="hU6 promoter" /ApEinfo_label="hU6 promoter" /ApEinfo_fwdcolor="#ff2fff" /ApEinfo_revcolor="#ff2fff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 31..63 /locus_tag="GS-linker" /label="GS-linker" /ApEinfo_label="GS-linker" /ApEinfo_fwdcolor="#8d8d8d" /ApEinfo_revcolor="#8d8d8d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 31..49 /locus_tag="M1-primer binding-site" /label="M1-primer binding-site" /ApEinfo_label="M1-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2012..2029) /locus_tag="M2-primer binding-site" /label="M2-primer binding-site" /ApEinfo_label="M2-primer binding-site" /ApEinfo_fwdcolor="#ff4f4f" /ApEinfo_revcolor="#ff4f4f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 GAACGCGGCC GCCAGCTGAA GCTTcgtacg TCAGGTGGAG GAGGTAGTGG CGGAGGCGGA 61 TCCaagagaa gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa 121 atctcatcct ccggggcact tgattatgat attccaacta ctgctagcga gaatttgtat 181 tttcagggtg agctcaaaac cgcggctctt gcgcaacacg atgaagccgt ggacaacaaa 241 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa 301 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt 361 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac 421 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa 481 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca 541 gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc cgcggggaag 601 tcaaccTGAA CTAGTaactt gtttattgca gcttataatg gttacaaata aagcaatagc 661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 721 ctcatcaatg tatcttaGTC GATATAACTT CGTATAGCAT ACATTATACG AAGTTATGTC 781 GACggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 841 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 901 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 1021 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 1081 gaggcctagg cttttgcaaa gaattcGCCA CCatggccaa gttgaccagt gccgttccgg 1141 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 1201 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 1261 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 1321 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 1381 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 1441 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaCTG CAGcgactgt 1501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 1561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 1621 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 1681 agacaatagc aggcatgctg gggatgcggt gggctctatg gCTCGAGgag ggcctatttc 1741 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta 1801 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc 1861 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa 1921 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccGATA 1981 ACTTCGTATA GCATACATTA TACGAAGTTA TGGTACCGAT GCAGCTAGCC CGCGGATCTG 2041 CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC AGGTTAACCT GCATTAATGA 2101 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 2161 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 2221 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 2281 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 2341 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 2401 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 2461 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA 2521 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 2581 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 2641 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 2701 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 2761 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 2821 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 2881 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 2941 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 3001 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 3061 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 3121 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 3181 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 3241 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 3301 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 3361 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 3421 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 3481 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 3541 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 3601 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 3661 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 3721 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 3781 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 3841 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 3901 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 3961 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 4021 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 4081 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 4141 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 4201 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 4261 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 4321 GTGCACCATA TGGACATATT GTCGTTAGAA CGCGGCTACA ATTAATACAT AACCTTATGT 4381 ATCATACACA TACGATTTAG GTGACACTAT A //