LOCUS       Exported                4372 bp ds-DNA     circular BCT 08-NOV-2018
DEFINITION  natural circular DNA
ACCESSION   pCV0049
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     S7942NS2_aph1_ccdB-SwaI
            NIL
            Category: Suicide plasmid for chromosomal integration / 
            Antibiotic(s): Km/Nm
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..6
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007"
     misc_feature    7..467
                     /label=S7942NSII-RA
                     /label=nonstandard type: recombination
                     /note="Right arm of the homologous recombination site NS2 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from GenBank: NC_007604, U44761"
                     /note="Andersson et al. (2000) Methods in enzymology 
                     305:527-42"
                     /note="/id=CVP091"
     misc_feature    376..381
                     /label=SacI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR005"
     misc_feature    478..1151
                     /label=ori-pBR322
                     /label=nonstandard type: ori_rep
                     /note="Origin of replication of pBR322"
                     /note="/id=CVP059"
     misc_feature    1324..1437
                     /label=bom
                     /label=nonstandard type: ori_tra
                     /note="bom site of pBR322, for comjugal transfer - requires
                     a helper plasmid such as pRL623 (or pRK2013)"
                     /note="/id=CVP061"
     misc_feature    1500..2441
                     /label=S7942NSII-LA
                     /label=nonstandard type: recombination
                     /note="Left arm of the homologous recombination site NS2 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from GenBank: NC_007604, U44761"
                     /note="Andersson et al. (2000) Methods in enzymology 
                     305:527-42"
                     /note="/id=CVP096"
     misc_feature    2442..2447
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004"
     misc_feature    2448..2468
                     /label=GC
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: BHR replicon and Neutral sites"
                     /note="/id=CVP005"
     misc_feature    2469..2474
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004-1"
     misc_feature    complement(2552..3367)
                     /label=aphI
                     /label=nonstandard type: ORF_antR
                     /note="Confers kanamycin resistance - aminoglycoside 
                     phosphotransferase, from GenBank: GU933126"
                     /note="Huang et al. (2010) Nucleic Acids Res. 38: 
                     2577-2593"
                     /note="/id=CVP026"
     misc_feature    complement(3368..3465)
                     /label=PaphI
                     /label=nonstandard type: promoter_antR
                     /note="Upstream region of aphI conserved between pPMAQK1 
                     and various plasmids, from GenBank: GU933126"
                     /note="Huang et al. (2010) Nucleic Acids Res. 38 (8), 
                     2577-2593"
                     /note="/id=CVP073"
     misc_feature    3474..3479
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001"
     misc_feature    3480..3500
                     /label=C2G
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Antibiotic resistance markers; 
                     Expression/reporter cassette, promoter-reporter module, 
                     custom sequence"
                     /note="/id=CVP006"
     misc_feature    3501..3506
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001-1"
     terminator      3526..3554
                     /label=lpp T
                     /note="lpp T - lpp terminator"
                     /note="Cao and Sarkar (1992) PNAS 89:7546-7550"
                     /note="/id=CVP099"
     misc_feature    3588..3595
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006"
     misc_feature    3632..3681
                     /label=PccdB
                     /label=nonstandard type: promoter_tox
                     /note="Upstream region of the ccdB ORF incl. promoter and 
                     RBS - from the destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP088"
     misc_feature    3937..4242
                     /label=ccdB
                     /label=nonstandard type: ORF_toxic
                     /note="From a destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP055"
     misc_feature    4283..4290
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006-1"
     misc_feature    4295..4300
                     /label=SacI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR005-1"
     terminator      4310..4338
                     /label=rrnB T2
                     /note="rrnB T2 - rrnB T2 terminator (partial sequence)."
                     /note="Orosz et al. (1991) Eur. J. Biochem. 201:653-659"
                     /note="/id=CVP100"
     misc_feature    4346..4351
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007-1"
     misc_feature    4352..4372
                     /label=G5C5
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Expression/reporter cassette, promoter-reporter 
                     module, custom sequence ; Cyanobacterial replicons"
                     /note="/id=CVP002"
ORIGIN
        1 tctagagttg ctaccaagta gtgacccgct tcgtgatgca aaatccgctg acgatattcg
       61 ggcgatcgct gctgaatgcc atcgagcagt aacgtggcac cccgcccctg ccaagtcacc
      121 gcatccagac tgaacagcac caagaggcta aaacccaatc ccgccggtag cagcggagaa
      181 ctacccagca ttggtcccac caaagctaat gccgtcgtgg taaaaatcgc gatcgccgtc
      241 agactcaagc ccagttcgct catgcttcct catctaggtc acagtcttcg gcgatcgcat
      301 cgatctgatg ctgcagcaag cgttttccat accggcgatc gcgccgtcgc cctttcgctg
      361 ccgtggcccg cttacgagct cgtttatcga ccacgatcgc atccaaatcc gcgatcgctt
      421 cccagtccgg caattcagtc tggggcgtcc gtttcattaa tcctgatagc gtcagacccc
      481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
      661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1141 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1201 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1261 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1321 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     1381 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     1441 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctccgcggg
     1501 ccacgatttg agggacgaat cttcagcgtg actgaagtac ctcgcggcaa gccatttccc
     1561 gatgtctttt tgttggccgc cgatcgcttc ggggttaatc ctacggcctg cgctgtgatc
     1621 gaagacaccc ccttgggagt agcggcaggc gtggcggcag gaatgcaagt gtttggctac
     1681 gcgggttcca tgcccgcttg gcgtctgcaa gaagccggtg cccatctcat ttttgacgat
     1741 atgcgactgc tgcccagtct gctccaatcg tcgccaaaag ataactccac agcattgccc
     1801 aatccctaac ccctgctcgc gccgcaacta cacactaaac cgttcctgcg cgatcgctct
     1861 tactgttgat ggctcgtgct taaaaacaat gcaaccctaa ccgtttcagc tggtgatttt
     1921 cggacgattt ggcttacagg gataactgag agtcaacagc ctctgtccgt cattgcacac
     1981 ccatccatgc actggggact tgactcatgc tgaatcacat ttcccttgtc cattgggcga
     2041 gaggggaggg gaatcttctg gactcttcac taagcggcga tcgcaggttc ttctacccaa
     2101 gcagtggcga tcgcttgatt gcagtcttca atgctggcct ctgcagccat cgccgccacc
     2161 aaagcatcgt aggcgggacg ttgttgctcc agtaaagtct tcgcccgtaa caatccccag
     2221 cgactgcgta aatccgcttc ggcaggattg cgatcgagtt gccgccacag ttgtttccac
     2281 tgggcgcgat cgtcagctcc cccttccacg ttgccgtaga ccagttgctc tgccgctgca
     2341 ccggccatca acacctgaca ccactgttcc agcgatcgct gactgagttg cccctgtgcg
     2401 gcttcggctt ctagcgcagc tgcttggaac tgcacacccc cgctagcatc gcgcgcgcgc
     2461 gcgcggacgc tagccaatgg cttattttcc tgctaattgg taataccatg gtaccgctct
     2521 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     2581 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     2641 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     2701 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2761 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     2821 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     2881 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     2941 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3001 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     3061 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     3121 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     3181 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     3241 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     3301 aatcagcatc catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat
     3361 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     3421 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggaccggta
     3481 tcccgccgcc gccgccggat accggttaat agtacctgtg aagtgaaaaa tggcgcacat
     3541 tgtgcgacat ttttttctcg agcaggtagt cggccaataa cccagggatt taaatacgcg
     3601 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     3661 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     3721 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     3781 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     3841 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     3901 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     3961 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     4021 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     4081 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     4141 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     4201 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     4261 cacagccagt ctgcaggtcg acatttaaat gaaggagctc cccggcagga gaaggccatc
     4321 ctgacggatg gcctttttga agctttctag aatcgggggc ccccgggggg at
//
