LOCUS       Exported                4705 bp ds-DNA     circular BCT 08-NOV-2018
DEFINITION  natural circular DNA
ACCESSION   pCV0053
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     S7942NS3-RK2BOM_aacC1_ccdB-SwaI
            NIL
            Category: Suicide plasmid for chromosomal integration / 
            Antibiotic(s): Gm
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..6
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007"
     misc_feature    7..808
                     /label=S7942NSIII-RA
                     /label=nonstandard type: recombination
                     /note="Right arm of the homologous recombination site NS3 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from pHN1_lacUV5, GenBank: NC_007604, HM026758"
                     /note="Niederholtmeyer et al. Appl. Environ. Microbiol. 
                     76:3462-3466"
                     /note="/id=CVP093"
     misc_feature    819..1492
                     /label=ori-pBR322
                     /label=nonstandard type: ori_rep
                     /note="Origin of replication of pBR322"
                     /note="/id=CVP059"
     misc_feature    1616..1919
                     /label=oriT
                     /label=nonstandard type: ori_tra
                     /note="oriT - streamlined origin of transfer from RP4 for 
                     conjugal transfer. PCR amplified from pRL692 (GenBank: 
                     AF424805)"
                     /note="Babic et al (2008) Research in Microbiology 
                     159:545-549; Koksharova and Wolk (2002) J. Bacteriol. 
                     184:5524-5528"
                     /note="/id=CVP062"
     misc_feature    1970..2861
                     /label=S7942NSIII-LA
                     /label=nonstandard type: recombination
                     /note="Left arm of the homologous recombination site NS3 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from pHN1_lacUV5, GenBank: NC_007604, HM026758"
                     /note="Niederholtmeyer et al. Appl. Environ. Microbiol. 
                     76:3462-3466"
                     /note="/id=CVP095"
     misc_feature    2862..2867
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004"
     misc_feature    2868..2888
                     /label=GC
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: BHR replicon and Neutral sites"
                     /note="/id=CVP005"
     misc_feature    2889..2894
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004-1"
     terminator      complement(2904..2930)
                     /label=trpA T
                     /note="trpA terminator from Lorist6 (GenBank: X98450), one 
                     nt shorter than reference"
                     /note="Gibson et al. (1987) Gene 53:283-286"
                     /note="/id=CVP098"
     misc_feature    complement(2938..3471)
                     /label=aacC1
                     /label=nonstandard type: ORF_antR
                     /note="Confers gentamycin resistance - gentamicin 
                     acetyltransferase - from pDAH346"
                     /note="Nair et al. (2001) J Bacteriol. 183:1740-1747"
                     /note="/id=CVP016"
     misc_feature    complement(3472..3806)
                     /label=PaacC1
                     /label=nonstandard type: promoter_antR
                     /note="Upstream region of aacC1 ORF incl. promoter and RBS 
                     - from pDAH346"
                     /note="Nair et al. (2001) J Bacteriol. 183:1740-1747"
                     /note="/id=CVP078"
     misc_feature    3807..3812
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001"
     misc_feature    3813..3833
                     /label=C2G
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Antibiotic resistance markers; 
                     Expression/reporter cassette, promoter-reporter module, 
                     custom sequence"
                     /note="/id=CVP006"
     misc_feature    3834..3839
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001-1"
     terminator      3859..3887
                     /label=lpp T
                     /note="lpp T - lpp terminator"
                     /note="Cao and Sarkar (1992) PNAS 89:7546-7550"
                     /note="/id=CVP099"
     misc_feature    3921..3928
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006"
     misc_feature    3965..4014
                     /label=PccdB
                     /label=nonstandard type: promoter_tox
                     /note="Upstream region of the ccdB ORF incl. promoter and 
                     RBS - from the destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP088"
     misc_feature    4270..4575
                     /label=ccdB
                     /label=nonstandard type: ORF_toxic
                     /note="From a destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP055"
     misc_feature    4616..4623
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006-1"
     misc_feature    4628..4633
                     /label=SacI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR005"
     terminator      4643..4671
                     /label=rrnB T2
                     /note="rrnB T2 - rrnB T2 terminator (partial sequence)."
                     /note="Orosz et al. (1991) Eur. J. Biochem. 201:653-659"
                     /note="/id=CVP100"
     misc_feature    4679..4684
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007-1"
     misc_feature    4685..4705
                     /label=G5C5
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Expression/reporter cassette, promoter-reporter 
                     module, custom sequence ; Cyanobacterial replicons"
                     /note="/id=CVP002"
ORIGIN
        1 tctagacgtg agccgtagtc ccgtcgccag acgcgggtgc ccacgggcgt cgtcaggatg
       61 tccgtaattg actgccggag gtggtcaatg cccttcagct ccttgccact gtcacggctc
      121 atgcctcggg tcattagtcg cccgctccgg tatcttcact ggcttcgatg attgccgccc
      181 cgcagctgca gaggtcaccg atccgagcag tcggcctctg gttggtaaag accgtgcgac
      241 tgccggtgat gatcgtgttc aagccatgca gggggcaggc gtgaaggtca tcctttcggg
      301 ccacggggcg gctgttgacg aaggtgtcgt cgctcccggt gatgatgatc ccgccgtgat
      361 cggtcacgtc gtttagtcga gcgatgcctg gcgtcgtagt cacgggttta ggtcaatacg
      421 acttgcggtc actgtaacgt tgccctcggc ggtcacgtta acgtcgcctt gggcttcgac
      481 ttgcgcctcc tgcacaagga tcacaatccg tccttgggct gcggtgaggt cgatcttgta
      541 ctcatgcgct tcgcggtcgt actggatgat tgagtcatcc tcgaactgcg tcttttggat
      601 cgtttctttg tcctcgatct gggggtagtc agtcgagaac gcgccgggca tcgcgaagcc
      661 ctgactgatc tcgccggagg gggccatcac gacgacggcc tcaccgacct cgggcgccca
      721 ccagaaccga tccttgcccg ctcgctgcgt gagccacgga atccagtcag tgaggagcag
      781 cgcctcgccg ctctcctcgt cttcctcgag cgtcagaccc cgtagaaaag atcaaaggat
      841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      961 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagactggt
     1621 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     1681 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     1741 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     1801 acaccaagga aagtctacgc gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     1861 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaagatcc
     1921 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctccgcggc gtcgtgatgt
     1981 cgaaacccaa gccaccctca gaggtgaagg ccgcttcgag cacatcggga agcgtgtcga
     2041 cgacggtgct cggtgcctcg ggtaagagcc agatagatgc ggtgacgttc accgtcgcga
     2101 tcgtggcggg aaccacggtc actgcgtccg ttacaactcg cacgtcgtcg gccagcacga
     2161 ctgactcgac ggcttcgatt agcccgggag aggcaaggcc atcaggctcg gtagacagaa
     2221 tgctgattaa cacctcgccg ggagcagggc tgctcacagc cgcgtccttc acccgtgggt
     2281 cagccgtcag cgcttgatag cggtaccagg ccgcaccgcc tgcggtcgag ctgcccttga
     2341 tccgctcgat ggtgcgatcg cgaagctcgc catccggctc attcggctgc cgcaccacgc
     2401 cgtagaacgc ggcaaggttg tcgaggtcgg ggccgtttga gtagcgaagc agtgtcgcaa
     2461 gaagtgcgtc gttgatccgc tgacgcagga tcagctcgcg ggctgcgcag acctccagca
     2521 gcttgatgac cgggtcggat tcgaggatgg cggtgtagct ggcgtcacgc gatcgcaggt
     2581 cgtcgatcaa gtcctgcagg atcagttcaa agtccagcgc ttcgatgatg gtgggcgcgg
     2641 gaatcgtagc aaagtcaaga acggtcatga gacgactaag ccctccagcg tgatacgcct
     2701 gccctcgggg atgtagtagc cgatcaggtt cagctcgact tgaccagctg cgctggctga
     2761 gacgatgcga accttctcca gcttcagccg tggctcccag cgatcgagcg cttcagctgt
     2821 ggccgccacc aggtcaacga tgagggactg gttgatcggt cgctagcatc gcgcgcgcgc
     2881 gcgcggacgc tagcggcgcg ccaaaaaaaa gcccgctcat taggcgggct ggccggctta
     2941 ggtggcggta cttgggtcaa tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     3001 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     3061 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     3121 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct
     3181 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     3241 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     3301 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     3361 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     3421 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     3481 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     3541 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     3601 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     3661 gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg
     3721 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga
     3781 acgagcgcaa ggtttcggtc tccacgaccg gtatcccgcc gccgccgccg gataccggtt
     3841 aatagtacct gtgaagtgaa aaatggcgca cattgtgcga catttttttc tcgagcaggt
     3901 agtcggccaa taacccaggg atttaaatac gcgtggatcc ggcttactaa aagccagata
     3961 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
     4021 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc
     4081 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
     4141 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
     4201 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
     4261 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     4321 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
     4381 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     4441 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     4501 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     4561 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgacattta
     4621 aatgaaggag ctccccggca ggagaaggcc atcctgacgg atggcctttt tgaagctttc
     4681 tagaatcggg ggcccccggg gggat
//
