LOCUS       Exported                5008 bp ds-DNA     circular BCT 08-NOV-2018
DEFINITION  natural circular DNA
ACCESSION   pCV0063
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     S7942NS1-RK2BOM_aadA_nt_ccdB-SwaI
            NIL
            Category: Suicide plasmid for chromosomal integration / 
            Antibiotic(s): SpSm, Cm
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..6
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007"
     misc_feature    7..786
                     /label=S7942NSI-RA
                     /label=nonstandard type: recombination
                     /note="Right arm of the homologous recombination site NS1 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from GenBank: NC_007604, U30252"
                     /note="Andersson et al. (2000) Methods in enzymology 
                     305:527-42"
                     /note="/id=CVP092"
     misc_feature    797..1470
                     /label=ori-pBR322
                     /label=nonstandard type: ori_rep
                     /note="Origin of replication of pBR322"
                     /note="/id=CVP059"
     misc_feature    1594..1897
                     /label=oriT
                     /label=nonstandard type: ori_tra
                     /note="oriT - streamlined origin of transfer from RP4 for 
                     conjugal transfer. PCR amplified from pRL692 (GenBank: 
                     AF424805)"
                     /note="Babic et al (2008) Research in Microbiology 
                     159:545-549; Koksharova and Wolk (2002) J. Bacteriol. 
                     184:5524-5528"
                     /note="/id=CVP062"
     misc_feature    1948..2818
                     /label=S7942NSI-LA
                     /label=nonstandard type: recombination
                     /note="Left arm of the homologous recombination site NS1 
                     into the chromosome of Synechococcus elongatus PCC7942 - 
                     from GenBank: NC_007604, U30252"
                     /note="Andersson et al. (2000) Methods in enzymology 
                     305:527-42"
                     /note="/id=CVP094"
     misc_feature    2537..2542
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007-1"
     misc_feature    2724..2729
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004"
     misc_feature    2784..2789
                     /label=EcoRV
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR002"
     misc_feature    2819..2824
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004-1"
     misc_feature    2825..2845
                     /label=GC
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: BHR replicon and Neutral sites"
                     /note="/id=CVP005"
     misc_feature    2846..2851
                     /label=NheI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR004-2"
     misc_feature    2852..3235
                     /label=PaadA
                     /label=nonstandard type: promoter_antR
                     /note="Upstream region of aadA ORF incl. promoter and RBS -
                     from pRL1383a (AF403426), originally from the omega 
                     interposon (GenBank: M60473)"
                     /note="Prentki et al. (1991) Gene 103:17-23"
                     /note="/id=CVP079"
     misc_feature    3236..4027
                     /label=aadA
                     /label=nonstandard type: ORF_antR
                     /note="Confers spectinomycin and/or streptomycin resistance
                     - aminoglycoside-3:adenyltransferase fusion - from pRL1383a
                     (AF403426), originally from the omega interposon (GenBank: 
                     M60473)"
                     /note="Prentki et al. (1991) Gene 103:17-23"
                     /note="/id=CVP024"
     misc_feature    4110..4115
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001"
     misc_feature    4116..4136
                     /label=C2G
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Antibiotic resistance markers; 
                     Expression/reporter cassette, promoter-reporter module, 
                     custom sequence"
                     /note="/id=CVP006"
     misc_feature    4137..4142
                     /label=AgeI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR001-1"
     terminator      4162..4190
                     /label=lpp T
                     /note="lpp T - lpp terminator"
                     /note="Cao and Sarkar (1992) PNAS 89:7546-7550"
                     /note="/id=CVP099"
     misc_feature    4224..4231
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006"
     misc_feature    4268..4317
                     /label=PccdB
                     /label=nonstandard type: promoter_tox
                     /note="Upstream region of the ccdB ORF incl. promoter and 
                     RBS - from the destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP088"
     misc_feature    4573..4878
                     /label=ccdB
                     /label=nonstandard type: ORF_toxic
                     /note="From a destination cassette used in the Gateway 
                     Technology developped by Life Technologies"
                     /note="Life Technologies"
                     /note="/id=CVP055"
     misc_feature    4919..4926
                     /label=SwaI
                     /label=nonstandard type: restrict_site
                     /note="NIL"
                     /note="/id=CVR006-1"
     misc_feature    4931..4936
                     /label=SacI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR005"
     terminator      4946..4974
                     /label=rrnB T2
                     /note="rrnB T2 - rrnB T2 terminator (partial sequence)."
                     /note="Orosz et al. (1991) Eur. J. Biochem. 201:653-659"
                     /note="/id=CVP100"
     misc_feature    4982..4987
                     /label=XbaI
                     /label=nonstandard type: restrict_site
                     /note="/id=CVR007-2"
     misc_feature    4988..5008
                     /label=G5C5
                     /label=nonstandard type: CV-adaptor
                     /note="Adaptor for the CyanoVECTOR assembly scheme, 
                     flanking: Expression/reporter cassette, promoter-reporter 
                     module, custom sequence ; Cyanobacterial replicons"
                     /note="/id=CVP002"
ORIGIN
        1 tctagattct ggagcaggaa gatgtcgcgg gcattagcac cagcggtctg ccaagcctcc
       61 gccagccgtt gggtcccttc cgcttgagct tttccatctt cgacgatacg ggcggcggcc
      121 ccccgcgctt ccgcgatcgc ccgtttacaa gctgcctcag ctggggcgat cacatcggct
      181 tgaagttgct gctgcacctg tttgatccgc tcctgctgca cagggagttc tgcttggcta
      241 cgagcgactt cggtagcaat gtccgcttca gcttcggcca ccaccgcttc gcgccgcgtc
      301 aacgcatcct gaatccggcg ctcggcctcg gcttgggcga tcgctacatc gcgatcgatc
      361 cgacgcaggg ccgtgatctt gtcattttcg gccgtttgga tcgcagaggc agcctgggca
      421 tcggcttcag caattcgggc atctcgctgc agatcagccc gctgcttgcg tccactagcc
      481 gagagataac cgacctcatc ggaaatgttc tggacttgca gcgtatcgag gactagaccc
      541 agctgctcaa ggtcatcctc cgcctcttcc agcagacttt tggcaaaggc aattttgtcc
      601 tcgttgatct gctccggcgt gaggctggct aaaacaccac gcaagttgcc ttcgagggtc
      661 tccttggcaa tttgctcgat ttccttacgg tttttgccaa gcagccgctc gatcgcgttg
      721 tggatggtcg gttcttcccc agcaatcttg atattggcaa cgccttcaac agtcagggga
      781 atgccgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      961 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1561 gcgcagcgag tcagtgagcg aggaagcgga agactggttg gcttggtttc atcagccatc
     1621 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     1681 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     1741 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacgcga
     1801 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     1861 ataatgaccc cgaagcaggg ttatgcagcg gaagatccct gacgggcttg tctgctcccg
     1921 gcatccgctt acagacaagc tccgcggcgg agcgctgctt tcttggcaag cggtcgccag
     1981 ccccaacgcc agggctgcca gcccgaaaca gcggggcaag gcagcttgga agggcgatcg
     2041 cagcacgggc atggcaatgt ctctctgaag gaatgcagac cttattcgta cagccagggt
     2101 tgaatcgtgg gggtccaatc acttagctct gctgggctaa accagagagc aatttcctgt
     2161 tgtgctgttt cgattgcatc cgagccatgg atgatgttgc ggccaatatt gacaccaaaa
     2221 tcaccacgga tggtgcccgg ttctgccgtc agcggattgg tagcgccgat caacttgcga
     2281 gcagccgcca caacgccttc gccttccaag acgatcgcca cgatcggccc agaggtgatg
     2341 aactcgacga ggccattgaa gaaggggcgc tcgcggtgga cagcatagtg ctgttcggcc
     2401 agctcgcgac tgggcttcag ctgctttagg cccaccagtt tgaagccttt ttgctcaaag
     2461 cggccgatga tcgtaccgac caaaccccgc tgaacgccat cgggcttgat ggcaataaat
     2521 gtgcgttcca cagacatcta gatagtcctc aagacgaggc aagcattgag cttgccttcc
     2581 tatggttcgg gatcactggg attcttgaca agcgatcgcg gtcacatcgc tatctcttag
     2641 gacttcgcag cgggcgagtc ggattgaccc ggtagggatt tcgccagatc aatgcccgtg
     2701 gtttgtttca gcttctccag caagctagcg atttgggtag cgctgccttc cccttcgcca
     2761 atcacagtga tcgactccac gtcgatatct ggcacggtgc ctgaaagcgt gacgagcagc
     2821 tagcatcgcg cgcgcgcgcg cggacgctag ctgcgtggag accgaaacct tgcgctcgtt
     2881 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     2941 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     3001 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     3061 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3121 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3181 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     3241 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3301 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3361 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3421 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3481 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3541 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3601 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3661 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3721 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3781 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3841 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3901 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3961 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     4021 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     4081 cgttagatgc actaagcaca taattgctca ccggtatccc gccgccgccg ccggataccg
     4141 gttaatagta cctgtgaagt gaaaaatggc gcacattgtg cgacattttt ttctcgagca
     4201 ggtagtcggc caataaccca gggatttaaa tacgcgtgga tccggcttac taaaagccag
     4261 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     4321 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     4381 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     4441 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     4501 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     4561 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     4621 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     4681 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     4741 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     4801 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     4861 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacat
     4921 ttaaatgaag gagctccccg gcaggagaag gccatcctga cggatggcct ttttgaagct
     4981 ttctagaatc gggggccccc ggggggat
//
