LOCUS       Exported                8136 bp ds-DNA     circular SYN 09-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   -
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     NIL
            -
            Category: - / Antibiotic(s): -
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(397..1248)
                     /label=repC
                     /label=nonstandard type: ORF_repl
                     /note="repC-RSF1010 - replication gene - from pRL1383a 
                     (GenBank:AF403426) , a derivative of RSF1010 
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP043"
     misc_feature    complement(1235..2074)
                     /label=repA
                     /label=nonstandard type: ORF_repl
                     /note="repA-RSF1010 - replication gene - from pRL1383a 
                     (GenBank:AF403426) , a derivative of RSF1010 
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP042"
     misc_feature    complement(2104..2310)
                     /label=repF
                     /label=nonstandard type: ORF_repl
                     /note="repF-RSF1010 - replication gene - from pRL1383a 
                     (GenBank:AF403426) , a derivative of RSF1010 
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP037"
     misc_feature    complement(2312..2524)
                     /label=repE
                     /label=nonstandard type: ORF_repl
                     /note="repE-RSF1010 - replication gene - from pRL1383a 
                     (GenBank:AF403426) , a derivative of RSF1010 
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP038"
     misc_feature    complement(2585..4714)
                     /label=mobA_Y25F
                     /label=nonstandard type: ORF_mob
                     /note="Mobilization gene - from pRL1383a (GenBank:AF403426)
                     , a derivative of RSF1010 (GenBank:M28829), point mutation 
                     Y25F to reduce the plasmid mobility by more than 99%."
                     /note="Scholz et al. (1989) Gene 75:271-288, Monzingo et 
                     al. (2007) J Mol Biol 366:165-78"
                     /note="/id=CVP036"
     misc_feature    complement(2585..3556)
                     /label=repB
                     /label=nonstandard type: ORF_repl
                     /note="repA-RSF1010 - replication gene - from pRL1383a 
                     (GenBank:AF403426) , a derivative of RSF1010 
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP044"
     misc_feature    complement(3553..3966)
                     /label=mobB
                     /label=nonstandard type: ORF_mob
                     /note="Mobilization gene - from pRL1383a (GenBank:AF403426)
                     , a derivative of RSF1010 (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP034"
     misc_feature    4913..5197
                     /label=mobC
                     /label=nonstandard type: ORF_mob
                     /note="Mobilization gene - from pRL1383a (AF403426) , a 
                     derivative of RSF1010 (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /note="/id=CVP033"
     misc_feature    5867..5887
                     /label=GC
                     /label=nonstandard type: GC-adaptor
                     /note="GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers or E. coli origins to be 
                     assembled with a cyanobacterial replicon."
                     /note="/id=CVP004"
     misc_feature    7122..7142
                     /label=C2G
                     /label=nonstandard type: GC-adaptor
                     /note="GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences."
                     /note="/id=CVP006"
     misc_feature    7324..8043
                     /label=nonstandard type: ORF_reporter
                     /note="""Genetic mutant of green fluorescent protein, 
                     derived from Aequorea victoria. Its excitation peak is 514"
                     /note="/id=CVP054"
     misc_feature    8116..8136
                     /label=G5C5
                     /label=nonstandard type: GC-adaptor
                     /note="GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons."
                     /note="/id=CVP002"
ORIGIN
        1 tctagaacaa tggaagtcct cgtctcgccc tcgaattagc ccgcctaatg agcgggcttt
       61 ttttgaatta attctcgcga gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtaa gttagcgcga attgcaagct ggccgacgcg ctgggctacg
      181 tcttgctggc gttcgggagc agaagagcat acatctggaa gcaaagccag gaaagcggcc
      241 tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta agccttttct
      301 gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt gaatataaaa
      361 gataaaaatg tcttgtttac aatagagtgg ggggggtcag cctgccgcct tgggccgggt
      421 gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga ccagctccgg
      481 caacgcctcg cgcacccgct tgcggcgctt gcgcatggtc gaaccactgg cctctgacgg
      541 ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg ggtcgatcca
      601 gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc gcacctcgtc
      661 catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca aggggttcag
      721 ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca gccgaaaccc
      781 ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct cgatgcagtc
      841 ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct ttgccagcgc
      901 ccgatagcta cctttgacca catggcattc agcggtgacg gcctcccact tgggttccag
      961 gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca ctaggccatt
     1021 aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc ccagcggctc
     1081 cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt ccagcttgct
     1141 gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac agtgcgccgg
     1201 gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg ggcaccccct
     1261 tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca tgccgcctga
     1321 accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag cggacgaaga
     1381 accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc atgctcgaca
     1441 ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg gcctgctgct
     1501 ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac acgatagagc
     1561 acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg gggccgctgg
     1621 cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg cggcggccct
     1681 cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc aggctgccga
     1741 tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg ctgaggtgcg
     1801 ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg ggcaggtaga
     1861 tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct gcaatctgtg
     1921 cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc agcgccccga
     1981 ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct gctgcgaacg
     2041 cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc aggcagttgg
     2101 tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt cttccaggca
     2161 ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac gcatcccttt
     2221 ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg ccagcaggtc
     2281 gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg ccggggccga
     2341 aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg ctggccatat
     2401 cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag ccaccgggca
     2461 accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt ttttcgtatt
     2521 ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag taccaacgca
     2581 agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg ggtgccggtg
     2641 cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc ttgctgacgg
     2701 tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct gggctggcct
     2761 cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc ttctgcgcgg
     2821 cgataaagtc gcacttgctg aggtcatgac cgaagcgctt gaccagcccg gccatctcgc
     2881 tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg ggcagttcga
     2941 ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc tgctggccag
     3001 cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca cgcagcagca
     3061 cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg aagcccgcca
     3121 agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg ctggccagcg
     3181 tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg acccatgcct
     3241 gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg gctttcatgt
     3301 catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc tcctgctcgg
     3361 cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat ggcgtgttct
     3421 ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtgggt gcgtccctga
     3481 cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg gcctgcaaag
     3541 tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct cggttgtcag
     3601 tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag gcatcatgga
     3661 agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc tccgggcgct
     3721 ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca gctccaccca
     3781 tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct cgctggcctg
     3841 cttggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc tctgggccag
     3901 cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc tgtcgattgc
     3961 gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg ttggcggtgt
     4021 cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc atctccacca
     4081 cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca agtgttctgt
     4141 ggtcaatgcg ggcgtcgtgg ccagcccgct ctaatgcccg gttggcatgg tcggcccatg
     4201 cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc tgtgccccgc
     4261 ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc cgctcgatgc
     4321 cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg ccaccggcat
     4381 ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg aactcggacg
     4441 ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg acctccttga
     4501 acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg gcgggccgct
     4561 cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg tcgcgggcat
     4621 acttgccttc gcgctggatg aagtcggcct tggccctggc cgattggccg cccgacctgc
     4681 tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc ttttgctttt
     4741 cggctccatg caatggccct cggagagcgc accgcccgaa gggtggccgt taggccagtt
     4801 tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg attgccgccg
     4861 ctgtgcctcc atgatagcct acgagacagc acattaacaa tggggtgtca agatggttaa
     4921 ggggagcaac aaggcggcgg atcggctggc caagctcgaa gaacaacgag cgcgaatcaa
     4981 tgccgaaatt cagcgggagc gggcaaggga acagcagcaa gagcgcaaga acgaaacaag
     5041 gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg aacagcagcg agtggccgga
     5101 ggatcggctc atggcggcaa tggatgcgta ccttgaacgc gaccacgacc gcgccttgtt
     5161 cggtctgccg ccacgccaga aggatgagcc gggctgaatg atcgaccgag acaggccctg
     5221 cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa agcggctaaa
     5281 agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt gcccgccttt
     5341 ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag accagctatc
     5401 cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc gctgataacc
     5461 gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc ccgccagccc
     5521 ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg ttcgtgacag
     5581 ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttagta cgggagtgac
     5641 gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt
     5701 ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg
     5761 tggcggccag gactcgcgag aattaattca gataaaaaaa atccttagct ttcgctaagg
     5821 atgatttcta gcgatgaccc tgctgattgg ttcgctgacc gctagcatcg cgcgcgcgcg
     5881 cgcggatgct agcggcgcgc caaaaaaaag cccgctcatt aggcgggctg gccggccttg
     5941 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     6001 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     6061 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     6121 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     6181 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     6241 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     6301 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     6361 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     6421 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     6481 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     6541 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     6601 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     6661 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     6721 ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     6781 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     6841 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     6901 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     6961 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt
     7021 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     7081 aacgagcgca aggtttcggt ctccacgcat tcgaaaccgg tatcccgccg ccgccgccgg
     7141 ataccggttt cgaattgaca attaatcatc ggctcgtata atggtaccgg tgataccagc
     7201 atcgtcttga tgcccttggc agcaccctgc taaggaggca acaagatgga acaaaaactg
     7261 attagcgaag aagatctgac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
     7321 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7381 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     7441 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     7501 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     7561 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     7621 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaggttcga gggcgacacc
     7681 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     7741 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     7801 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     7861 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     7921 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     7981 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8041 taacatgagc cgtctcgacc tcggccctgc aggagaaggc catcctgacg gatggccttt
     8101 ttgaagcttt ctagaatcgg gggcccccgg ggggat
//
