LOCUS       pAM5187\(pAM4787        6194 bp    DNA     circular      2-SEP-2014
DEFINITION  Cloning vector pUC19, complete sequence.
SOURCE      Cloning vector pUC19.
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     See also GenBank accession L09137.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|708003874|
COMMENT     VNTDBDATE|708007954|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAM5187 (pAM4787-MCS-pBS_sb1-2)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "DNA 'pAM5187 (pAM4787-MCS-pBS_sb1-2)'" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Cloning vector pUC19, complete sequence." 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pUC19\">Cloning vector pUC19(Cloning vector pUC19)</a>" 
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FEATURES             Location/Qualifiers
     CDS             complement(1975..2448)
                     /gene="repB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB21874.1"
                     /db_xref="GI:247791"
                     /vntifkey="4"
                     /label=repB
                     /note="#1 amino acid V to M"
     CDS             complement(2403..5033)
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB21875.1"
                     /db_xref="GI:247792"
                     /vntifkey="4"
                     /label=repA
     CDS             complement(5209..5536)
                     /gene="orf G"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="AAB21876.1"
                     /db_xref="GI:247793"
                     /vntifkey="4"
                     /label=orf\G
                     /note="mismatch(1[M->V])"
     misc_feature    5543..5563
                     /vntifkey="21"
                     /label=C3G3
     misc_marker     402..407
                     /vntifkey="22"
                     /label=NheI
     misc_marker     5564..5569
                     /vntifkey="22"
                     /label=MfeI
     misc_feature    complement(232..345)
                     /gene="bom"
                     /vntifkey="21"
                     /label=bom
     rep_origin      1..1
                     /vntifkey="33"
                     /label=ori
     CDS             complement(460..1275)
                     /vntifkey="4"
                     /label=aadAnew
     promoter        complement(1271..1650)
                     /vntifkey="30"
                     /label=aadA
     terminator      complement(443..470)
                     /vntifkey="43"
                     /label=cat\3'UTR
     misc_feature    complement(408..428)
                     /vntifkey="21"
                     /label=GC
     misc_structure  5564..407
                     /vntifkey="88"
                     /label=pBR322\oriV\oriT\fragment
     misc_structure  408..1662
                     /vntifkey="88"
                     /label=C2G-aadAnew-GC_EcoRV
     primer          1635..1654
                     /vntifkey="27"
                     /label=YC011
                     /note="YC011-aadA-pro-5c"
     primer          complement(5669..5689)
                     /vntifkey="27"
                     /label=YC055
                     /note="YC055-pBR322-ori-5c"
     primer          5209..5228
                     /vntifkey="27"
                     /label=YC056
                     /note="YC056-pANS-orfG-C3"
     primer          complement(1974..1995)
                     /vntifkey="27"
                     /label=YC057
                     /note="YC057-pANS-repB-c3d"
     rep_origin      complement(5583..56)
                     /vntifkey="33"
                     /label=pMB1\origin
     primer          6129..6149
                     /vntifkey="27"
                     /label=YC072
                     /note="YC072-pMB1-origin-3d"
     primer          159..183
                     /vntifkey="27"
                     /label=ta098
                     /note="ta098_1303BR"
     misc_marker     429..434
                     /vntifkey="22"
                     /label=NheI
     misc_marker     435..442
                     /vntifkey="22"
                     /label=AscI
     misc_marker     471..478
                     /vntifkey="22"
                     /label=FseI
     misc_marker     1651..1656
                     /vntifkey="22"
                     /label=BstBI
     misc_marker     1657..1662
                     /vntifkey="22"
                     /label=AgeI
     misc_marker     5537..5542
                     /vntifkey="22"
                     /label=SacI
     primer          complement(587..608)
                     /vntifkey="27"
                     /label=yc075
     primer          1467..1484
                     /vntifkey="27"
                     /label=yc076
     primer          complement(440..462)
                     /vntifkey="27"
                     /label=yc163
     misc_feature    1663..1770
                     /vntifkey="21"
                     /label=MCS_pBS
     primer          1643..1679
                     /vntifkey="27"
                     /label=yc336
     primer          complement(1753..1790)
                     /vntifkey="27"
                     /label=yc337
     misc_marker     1765..1770
                     /vntifkey="22"
                     /label=KpnI
     misc_marker     1663..1668
                     /vntifkey="22"
                     /label=SacI
BASE COUNT     1231 a      1822 c      1787 g      1354 t 
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgctagcatc gcgcgcgcgc 
      421 gcgcggatgc tagcggcgcg ccaaaaaaaa gcccgctcat taggcgggct ggccggcctt 
      481 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc 
      541 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac 
      601 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc 
      661 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc 
      721 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa 
      781 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac 
      841 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc 
      901 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc 
      961 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag 
     1021 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg 
     1081 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt 
     1141 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg 
     1201 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc 
     1261 cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct 
     1321 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat 
     1381 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc 
     1441 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta 
     1501 cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg 
     1561 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc 
     1621 gaacgagcgc aaggtttcgg tctccacgca ttcgaaaccg gtgagctcca ccgcggtggc 
     1681 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat 
     1741 accgtcgacc tcgagggggg gcccggtacc ctagaccgag ctgggcactg acagcctcag 
     1801 tgagcggctc tcggaagcgc tcgagcattg gccacatgtg cttgggtgct gcgatggcca 
     1861 gcgttccact atcgaagctg taggcgcggc agcggttagc cgcaaaccat cgcaagcctt 
     1921 cgttctcgat cgcggcgatc gcagttgtcc aaagctgatc gagttcggct tgtgtcatgc 
     1981 tgcggtctcc gtagatgtag tgctgacctg acctgacgcg gtgtcacgaa aatgcgcagt 
     2041 gattggtttt gatcttctat tagggatttc gtgacagctc cccagtccct gactagatcg 
     2101 ggcgttttca agccaatgag cttcgatcgc ctcaagcggc agttcagcaa tcgcctcatt 
     2161 cggcacgact tggtagatcc aagtggactt gcccgcgatg cgcttctgcc gtgccttgag 
     2221 cttgaagccg atcatccgca gcagcgcccg cacgatggtg ctggctctgg tcttgtcggt 
     2281 gatctgaatc ttgaggatct gtgcggcact agcccggtgg cttgtggcgg tcgtgacgat 
     2341 ctcttgaatc agcggatcat caacgctgaa ccagtcgcgg cgttgcagca actcttccac 
     2401 cttcagccca tgcaggtacc gcacttgggg cgcgatacag ctgcccacaa gatcgggtcc 
     2461 gaacatcggg cgatcgcgcc gtttgtcgag cttggcggca tcgcgagcgg cacggcatcc 
     2521 ttgccaaggc tgagccagaa cagcagtctt agcggggcgt atagaccgtc gtcatcagcg 
     2581 gctagtagtt cacgggtgca gtctccccag cgttgctggc agcggtgccg agcaagggca 
     2641 gccaactgtt ccgcgttagc gaagtccgca gacgcgatcg cctcagcttc cttcggggtc 
     2701 agatcaggtg aactcaggat cgcctcggct gaggcatcag ccagcgcttc agcagtctcc 
     2761 cgttgcagtt cgtcaacgac ctcttgaaga aagggatcga tcgcgccgtc ccagtcttgc 
     2821 caagtgaacc cgttgagccg tagaagctgc tccaccgtgg cggcataggt caacccgtca 
     2881 cggttgcgac gggcggccaa ttctgcccat gcagtcaagt aggcttgcga tgcgaccggc 
     2941 tcgccgatct tgatcgccgc ctgtgacagc tgagccaaga tcacttgttg gtgccgcttc 
     3001 aggccgtcgg caacctcggt tgggtcgatg ctaccgttgc cagctcgcat caaattggac 
     3061 gtgtcggggc aggtgatgaa tcgggggacg ttgctccgca cccgtcctgc aatctgcgcg 
     3121 atcgcacctg gggcgatgtg cccagctccg gcgaagatca gcacggcatc aaatgggcga 
     3181 tcgcctaccc cttgggtgat cgacaagcca gcatcgatca cgctcgtgaa gacaaggtgg 
     3241 gggcaacagt tgatgaacat ctgcgggttg agcttcagcg tttccaaagc cccccgctta 
     3301 ttggcttcgc tgtcgatgac cggaaccggc tgaccgcagg cttcggagag ctgttgggcg 
     3361 atcgtcgctg cggaatacag ggacggccgc gactggctca gcttctggcg gtcgcagaag 
     3421 actgctaagc gcttgcccgc ctgaaccatt tcgaccgcca tcgccatgag ttcgcgatcg 
     3481 ctgccaacct gatagaccgg agcaccgctg aagggttgga cggtactgct gaccagatat 
     3541 gcggttgatc ctgtcagtgc ttccaacagg gcgatcgaca catccgacag catcgcgtca 
     3601 gcggcgataa cctgccgccc ccctgcgagt agtccagcaa tttcagccat gacttcgggg 
     3661 cgatgtttag cgatctcggt cttgccgctc agcaggtgct cagcccacag ttccacctca 
     3721 tcgatgacga cgatcgcccg ttgccatccg tgggcgctga agcgcagctt gctggcgggg 
     3781 tgcatggagt ctaggcaacc gccaaggccg atcatccgaa ggtcggaacc gggaacgcat 
     3841 tcttcagccc acggcacccc taagcgcttg cccaaggccg cgcccaaggt acggcgatgc 
     3901 gtgatcaaca cgacaggaat gccggtacca atgagcggct ggagatgccc cgcgatcgcc 
     3961 tctgtcttgc ccgtccccat cgcagcattc aggcagacaa ggcgatgctg ttccggctgc 
     4021 gggatgtagt cgccgttgat gtacccgccg gtgtgtaagc ggttagggct acgggtcagc 
     4081 cgctgccact cgttcgcgac ctcgcgctga tattcccgct gctcttgtcg agtcagcttt 
     4141 ggcgggcggt agccgccttc tttggccagc ttggccagtg tggcgatcgt gatgccgctg 
     4201 cccttgaagc tggcccactt ccgctctagc ttgcggttgc cgtcgaactt ggacgattgc 
     4261 gctgaccatg caatccagtc atctaacagg gcatcgctga ctgactgcag ggccatcccg 
     4321 acagccaccc agctctcgta gtcgtctgcg tagctggcag gtacatgggc cagcatggtg 
     4381 cgggcgatcg cgacctcatc atcagaagct ggagcactga cgatcggctt ataggatttc 
     4441 ggcgcggtct gccggtcttg ccgctccatt gcatccagca attcgggcgg ggcctctgca 
     4501 acctcgatct cccaaggtgc gcagccttcc acccagcgat atgcgccggt ctcagggtgt 
     4561 tgaccaacga tgactgattg cagcttgttc caacgcagct ctaacagatc accgtctggg 
     4621 ctgtgaagct tctgcgtggc gatcgcagcc catcgctctt ggggtacctg atagagccgt 
     4681 tggtggcgtc ctggcttgcc agatgtatat gcaactgtgg gcggtaattc tgtcagcgtc 
     4741 agcccttctg gcagtgcctc atgagccgtt gggccatcga agtcaactgc gattaaccca 
     4801 ccgcttgcgg ggccagtaag cagaccgatt ccggtaacgc gccgatcgct gcggatacgt 
     4861 tcagcaacag cagcatggtc aagcgggtta ttttgccagc cagggtcaaa tggtgccttg 
     4921 cgactgttaa cgacgcagaa gcgccagttt gggggcagcc ggtcaagttg atcaattaat 
     4981 tcgcagcggg tcgggatctg cgacctttgc agatgattgg cgataaatgc catacttctg 
     5041 ttgagttatt tttcctgacc tcccctgggc aaagtttgcc gacctggcct ggggggggtt 
     5101 ttgctttgat tgccgataac tatagctgga atgaatacgg caggtggcaa ggctgcctcg 
     5161 aattgatcgg caagccagag gcgcgatcgc ccctggctgt gatgagactt agacgccgga 
     5221 cgctgtcgag atccgaacga tcggacggtg atcgactacg cagcagccga agctgtgctc 
     5281 agccttggcg cggtaaaagc ccgtctcgaa ctgcacctcg ctagttagct gaggcgcggg 
     5341 cgtcaggcgt cccagctcca gaggtaggtt gccgcgtgca actaggtagt agtagctgcc 
     5401 gctcagccgt gcgtcgatcg caacctccag cgaccaaggg ttgaactcat cgcggctgtt 
     5461 gacttgcaga tctgccacca actgacggat aggcagctca ctctcaggcg acgcgaggat 
     5521 cagtccaggc tcttgagagc tcatccccgg gcccgggccc gatcaattga gcgtcagacc 
     5581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     5641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     5701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 
     5761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     5821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     5881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     5941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     6001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     6061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     6121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     6181 ggagcctatg gaaa 
//
