LOCUS       pAM5188\(pAM4788        6936 bp    DNA     circular     22-DEC-2014
DEFINITION  Cloning vector pUC19, complete sequence.
SOURCE      Cloning vector pUC19.
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     See also GenBank accession L09137.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|718032923|
COMMENT     VNTDBDATE|718033271|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAM5188 (pAM4788-MCS-pBS_sb2-1)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #43=(CFSignal (CObList) "KpnI" 22 0 0 1765 1770 0 (CStringList) 
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COMMENT                          "DNA 'pAM5188 (pAM4788-MCS-pBS_sb2-1)'" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Cloning vector pUC19, complete sequence." 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/22/14 01:35PM" 1) 
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COMMENT                          "Last Modified: 12/22/14 01:41PM" 1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6936 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-OCT-2007" 1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: Cloning vector pUC19" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pUC19\">Cloning vector pUC19(Cloning vector pUC19)</a>" 
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COMMENT                          "Taxonomy: other sequences; artificial sequences; vectors" 
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COMMENT                          "Associated Genes:  bla;  repB;  repA;  orf G" 1))) 
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COMMENT                          "  AUTHORS   New England Biolabs." 1) 
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COMMENT                          "            240 County Road, Ipswich, MA 01938, USA" 
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FEATURES             Location/Qualifiers
     misc_marker     1771..1775
                     /vntifkey="22"
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(1975..2448)
                     /gene="repB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB21874.1"
                     /db_xref="GI:247791"
                     /vntifkey="4"
                     /label=repB
                     /note="#1 amino acid V to M"
     CDS             complement(2403..5033)
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB21875.1"
                     /db_xref="GI:247792"
                     /vntifkey="4"
                     /label=repA
     CDS             complement(5209..5536)
                     /gene="orf G"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="AAB21876.1"
                     /db_xref="GI:247793"
                     /vntifkey="4"
                     /label=orf\G\partial
                     /note="mismatch(1[M->V])"
     misc_marker     6279..6284
                     /vntifkey="22"
                     /label=SacI
                     /note="SacI"
     misc_feature    6285..6305
                     /vntifkey="21"
                     /label=C3G3
     misc_marker     402..407
                     /vntifkey="22"
                     /label=NheI
     misc_marker     6306..6311
                     /vntifkey="22"
                     /label=MfeI
     misc_feature    complement(232..345)
                     /gene="bom"
                     /vntifkey="21"
                     /label=bom
     rep_origin      1..1
                     /vntifkey="33"
                     /label=ori
     CDS             complement(479..1275)
                     /vntifkey="4"
                     /label=aadA_new
     promoter        complement(1271..1650)
                     /vntifkey="30"
                     /label=Promoter_P_1
     misc_feature    complement(443..470)
                     /vntifkey="21"
                     /label=cat\3'UTR
     misc_feature    complement(408..428)
                     /vntifkey="21"
                     /label=GC
     misc_structure  6306..407
                     /vntifkey="88"
                     /label=pBR322\oriV\oriT\fragment
     misc_structure  408..1662
                     /vntifkey="88"
                     /label=aadA-new\fragment
     primer          1635..1654
                     /vntifkey="27"
                     /label=YC011
                     /note="YC011-aadA-pro-5c"
     primer          complement(6411..6431)
                     /vntifkey="27"
                     /label=YC055
                     /note="YC055-pBR322-ori-5c"
     primer          5209..5228
                     /vntifkey="27"
                     /label=YC056
                     /note="YC056-pANS-orfG-C3"
     primer          complement(1974..1995)
                     /vntifkey="27"
                     /label=YC057
                     /note="YC057-pANS-repB-c3d"
     conflict        1891..1893
                     /vntifkey="83"
                     /label=AGC
     conflict        5180..5182
                     /vntifkey="83"
                     /label=GGC
     rep_origin      complement(6325..56)
                     /vntifkey="33"
                     /label=pMB1\origin
     primer          6871..6891
                     /vntifkey="27"
                     /label=YC072
                     /note="YC072-pMB1-origin-3d"
     primer          159..183
                     /vntifkey="27"
                     /label=ta098
                     /note="ta098_1303BR"
     misc_marker     429..434
                     /vntifkey="22"
                     /label=NheI
     misc_marker     435..442
                     /vntifkey="22"
                     /label=AscI
     misc_marker     471..478
                     /vntifkey="22"
                     /label=FseI
     misc_marker     1651..1656
                     /vntifkey="22"
                     /label=BstBI
     misc_marker     1657..1662
                     /vntifkey="22"
                     /label=AgeI
     misc_marker     5537..5542
                     /vntifkey="22"
                     /label=SacI
     CDS             5645..5878
                     /locus_tag="ssl7004"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_942292.1"
                     /db_xref="GI:38505672"
                     /db_xref="GeneID:2656047"
                     /vntifkey="4"
                     /label=ssl7004
     CDS             5850..6278
                     /locus_tag="sll7003"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
                     /protein_id="NP_942291.1"
                     /db_xref="GI:38505671"
                     /db_xref="GeneID:2656038"
                     /vntifkey="4"
                     /label=sll7003
     misc_signal     5603..5603
                     /vntifkey="87"
                     /label=TSS
     -10_signal      5591..5596
                     /vntifkey="47"
                     /label=-10
     -35_signal      5571..5576
                     /vntifkey="48"
                     /label=-35
     misc_structure  5543..6278
                     /vntifkey="88"
                     /label=pSYSA-TA1\cassette
     primer          5517..5565
                     /vntifkey="27"
                     /label=YC087
                     /note="YC087-orfG-ssl7004-5d"
     primer          complement(6257..6305)
                     /vntifkey="27"
                     /label=YC088
                     /note="YC088-C3G3-ssl7004-3c"
     primer          complement(587..608)
                     /vntifkey="27"
                     /label=yc075
     primer          complement(440..462)
                     /vntifkey="27"
                     /label=yc163
     primer          5879..5903
                     /vntifkey="27"
                     /label=yc248
     misc_feature    1663..1770
                     /vntifkey="21"
                     /label=MCS_pBS
     misc_marker     1765..1770
                     /vntifkey="22"
                     /label=KpnI
     misc_marker     1663..1668
                     /vntifkey="22"
                     /label=SacI
     primer          1643..1679
                     /vntifkey="27"
                     /label=yc336
     primer          complement(1753..1790)
                     /vntifkey="27"
                     /label=yc337
BASE COUNT     1433 a      1982 c      1975 g      1546 t 
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgctagcatc gcgcgcgcgc 
      421 gcgcggatgc tagcggcgcg ccaaaaaaaa gcccgctcat taggcgggct ggccggcctt 
      481 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc 
      541 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac 
      601 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc 
      661 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc 
      721 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa 
      781 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac 
      841 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc 
      901 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc 
      961 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag 
     1021 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg 
     1081 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt 
     1141 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg 
     1201 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc 
     1261 cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct 
     1321 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat 
     1381 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc 
     1441 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta 
     1501 cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg 
     1561 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc 
     1621 gaacgagcgc aaggtttcgg tctccacgca ttcgaaaccg gtgagctcca ccgcggtggc 
     1681 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat 
     1741 accgtcgacc tcgagggggg gcccggtacc ctagaccgag ctgggcactg acagcctcag 
     1801 tgagcggctc tcggaagcgc tcgagcattg gccacatgtg cttgggtgct gcgatggcca 
     1861 gcgttccact atcgaagctg taggcgcggc agcggttagc cgcaaaccat cgcaagcctt 
     1921 cgttctcgat cgcggcgatc gcagttgtcc aaagctgatc gagttcggct tgtgtcatgc 
     1981 tgcggtctcc gtagatgtag tgctgacctg acctgacgcg gtgtcacgaa aatgcgcagt 
     2041 gattggtttt gatcttctat tagggatttc gtgacagctc cccagtccct gactagatcg 
     2101 ggcgttttca agccaatgag cttcgatcgc ctcaagcggc agttcagcaa tcgcctcatt 
     2161 cggcacgact tggtagatcc aagtggactt gcccgcgatg cgcttctgcc gtgccttgag 
     2221 cttgaagccg atcatccgca gcagcgcccg cacgatggtg ctggctctgg tcttgtcggt 
     2281 gatctgaatc ttgaggatct gtgcggcact agcccggtgg cttgtggcgg tcgtgacgat 
     2341 ctcttgaatc agcggatcat caacgctgaa ccagtcgcgg cgttgcagca actcttccac 
     2401 cttcagccca tgcaggtacc gcacttgggg cgcgatacag ctgcccacaa gatcgggtcc 
     2461 gaacatcggg cgatcgcgcc gtttgtcgag cttggcggca tcgcgagcgg cacggcatcc 
     2521 ttgccaaggc tgagccagaa cagcagtctt agcggggcgt atagaccgtc gtcatcagcg 
     2581 gctagtagtt cacgggtgca gtctccccag cgttgctggc agcggtgccg agcaagggca 
     2641 gccaactgtt ccgcgttagc gaagtccgca gacgcgatcg cctcagcttc cttcggggtc 
     2701 agatcaggtg aactcaggat cgcctcggct gaggcatcag ccagcgcttc agcagtctcc 
     2761 cgttgcagtt cgtcaacgac ctcttgaaga aagggatcga tcgcgccgtc ccagtcttgc 
     2821 caagtgaacc cgttgagccg tagaagctgc tccaccgtgg cggcataggt caacccgtca 
     2881 cggttgcgac gggcggccaa ttctgcccat gcagtcaagt aggcttgcga tgcgaccggc 
     2941 tcgccgatct tgatcgccgc ctgtgacagc tgagccaaga tcacttgttg gtgccgcttc 
     3001 aggccgtcgg caacctcggt tgggtcgatg ctaccgttgc cagctcgcat caaattggac 
     3061 gtgtcggggc aggtgatgaa tcgggggacg ttgctccgca cccgtcctgc aatctgcgcg 
     3121 atcgcacctg gggcgatgtg cccagctccg gcgaagatca gcacggcatc aaatgggcga 
     3181 tcgcctaccc cttgggtgat cgacaagcca gcatcgatca cgctcgtgaa gacaaggtgg 
     3241 gggcaacagt tgatgaacat ctgcgggttg agcttcagcg tttccaaagc cccccgctta 
     3301 ttggcttcgc tgtcgatgac cggaaccggc tgaccgcagg cttcggagag ctgttgggcg 
     3361 atcgtcgctg cggaatacag ggacggccgc gactggctca gcttctggcg gtcgcagaag 
     3421 actgctaagc gcttgcccgc ctgaaccatt tcgaccgcca tcgccatgag ttcgcgatcg 
     3481 ctgccaacct gatagaccgg agcaccgctg aagggttgga cggtactgct gaccagatat 
     3541 gcggttgatc ctgtcagtgc ttccaacagg gcgatcgaca catccgacag catcgcgtca 
     3601 gcggcgataa cctgccgccc ccctgcgagt agtccagcaa tttcagccat gacttcgggg 
     3661 cgatgtttag cgatctcggt cttgccgctc agcaggtgct cagcccacag ttccacctca 
     3721 tcgatgacga cgatcgcccg ttgccatccg tgggcgctga agcgcagctt gctggcgggg 
     3781 tgcatggagt ctaggcaacc gccaaggccg atcatccgaa ggtcggaacc gggaacgcat 
     3841 tcttcagccc acggcacccc taagcgcttg cccaaggccg cgcccaaggt acggcgatgc 
     3901 gtgatcaaca cgacaggaat gccggtacca atgagcggct ggagatgccc cgcgatcgcc 
     3961 tctgtcttgc ccgtccccat cgcagcattc aggcagacaa ggcgatgctg ttccggctgc 
     4021 gggatgtagt cgccgttgat gtacccgccg gtgtgtaagc ggttagggct acgggtcagc 
     4081 cgctgccact cgttcgcgac ctcgcgctga tattcccgct gctcttgtcg agtcagcttt 
     4141 ggcgggcggt agccgccttc tttggccagc ttggccagtg tggcgatcgt gatgccgctg 
     4201 cccttgaagc tggcccactt ccgctctagc ttgcggttgc cgtcgaactt ggacgattgc 
     4261 gctgaccatg caatccagtc atctaacagg gcatcgctga ctgactgcag ggccatcccg 
     4321 acagccaccc agctctcgta gtcgtctgcg tagctggcag gtacatgggc cagcatggtg 
     4381 cgggcgatcg cgacctcatc atcagaagct ggagcactga cgatcggctt ataggatttc 
     4441 ggcgcggtct gccggtcttg ccgctccatt gcatccagca attcgggcgg ggcctctgca 
     4501 acctcgatct cccaaggtgc gcagccttcc acccagcgat atgcgccggt ctcagggtgt 
     4561 tgaccaacga tgactgattg cagcttgttc caacgcagct ctaacagatc accgtctggg 
     4621 ctgtgaagct tctgcgtggc gatcgcagcc catcgctctt ggggtacctg atagagccgt 
     4681 tggtggcgtc ctggcttgcc agatgtatat gcaactgtgg gcggtaattc tgtcagcgtc 
     4741 agcccttctg gcagtgcctc atgagccgtt gggccatcga agtcaactgc gattaaccca 
     4801 ccgcttgcgg ggccagtaag cagaccgatt ccggtaacgc gccgatcgct gcggatacgt 
     4861 tcagcaacag cagcatggtc aagcgggtta ttttgccagc cagggtcaaa tggtgccttg 
     4921 cgactgttaa cgacgcagaa gcgccagttt gggggcagcc ggtcaagttg atcaattaat 
     4981 tcgcagcggg tcgggatctg cgacctttgc agatgattgg cgataaatgc catacttctg 
     5041 ttgagttatt tttcctgacc tcccctgggc aaagtttgcc gacctggcct ggggggggtt 
     5101 ttgctttgat tgccgataac tatagctgga atgaatacgg caggtggcaa ggctgcctcg 
     5161 aattgatcgg caagccagag gcgcgatcgc ccctggctgt gatgagactt agacgccgga 
     5221 cgctgtcgag atccgaacga tcggacggtg atcgactacg cagcagccga agctgtgctc 
     5281 agccttggcg cggtaaaagc ccgtctcgaa ctgcacctcg ctagttagct gaggcgcggg 
     5341 cgtcaggcgt cccagctcca gaggtaggtt gccgcgtgca actaggtagt agtagctgcc 
     5401 gctcagccgt gcgtcgatcg caacctccag cgaccaaggg ttgaactcat cgcggctgtt 
     5461 gacttgcaga tctgccacca actgacggat aggcagctca ctctcaggcg acgcgaggat 
     5521 cagtccaggc tcttgagagc tctcgttcat acacaagcag ggcaggcaaa tgatcaagcg 
     5581 ctatcaatgc tatcatttga gtaatgacca agaccaagag tttgttttgg gctggaggta 
     5641 gtggatggct aatttaattg ttcgcaatat tgatgaagcg gtggtggtgg ccctgaaaaa 
     5701 gcgggcgggg caacatggca ttagtgccga agcagaacat cgcaaaatcc tagagcaggc 
     5761 cctattacga cttccccgaa agccctttgc cgaggtgctc cgccagattc ccaatgtggg 
     5821 caatgactca gattttgagc gaatacagga tgacagagcc agtcaaatat ttgattgata 
     5881 ccaacgttat tagtgagcta cggaaacaag atagggccga ccccggtgta ctagcatttt 
     5941 ttcagcaagc tcagcatctc tatttgagtg tgattaccat tggggaattg cggcggggtg 
     6001 tggaattaat caggcatcgg ggagatgttc aacaagccaa tttactggag gagtggttgg 
     6061 aaattgtaat tgctgattac gctgaccaca tcctagattt caccatcttc gaggctcaag 
     6121 ttttgggaaa gttgcgggtt cctaatcccc ataacgccct agataaacaa attgccgcca 
     6181 ctgcccttag ttatggactg accctagtta ctcgcaatgt caaggatttt gccgacacag 
     6241 gagtttgttt gcttaatcct ttcaccggca atcaataaga gctcatcccc gggcccgggc 
     6301 ccgatcaatt gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     6481 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaa 
//
