LOCUS       Exported               10507 bp ds-DNA     circular SYN 31-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Liza Brusman
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3425
                     /label=Itprip C-term V5 LIC F
     misc_feature    3391..3401
                     /label=C-term LIC
     misc_feature    3402..3410
                     /label=Kozak sequence
     misc_feature    3408..5048
                     /label=ITPRIP Transcript variant 1 (correct)
     primer_bind     complement(5029..5065)
                     /label=Itprip C-term V5 LIC R
     misc_feature    5049..5051
                     /label=from primer
     misc_feature    5052..5065
                     /label=C-term LIC
     misc_feature    5058..5075
                     /label=6X Gly
     CDS             5076..5117
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5147..5805
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(5326..5345)
                     /label=IRES-R Addgene
     primer_bind     complement(5398..5415)
                     /label=IRES-R Jakki
     CDS             5806..6405
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6429..7020
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(9145..10005)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gccatggggc
     3421 tcttccgcgt gtgtctggtg gtggtgacgg ccatcatcaa ccacccgctg ctgttcccgc
     3481 gggagaacgc cacagtcccc gagaacgagg aggagatcat ccgcaagatg caggcgcacc
     3541 aggagaagct gcagctggag cagttgcgcc tggaggagga ggtggctcgg ctggcggccg
     3601 aaaaggaggc actggagcag gtggcggagg agggcaggca gcagaacgag acacgcgtgg
     3661 cctgggacct ctggagcacc ctctgcatga tcctcttcct gatgatcgag gtgtggcggc
     3721 aggaccacca ggaggggccc tcacctgagt gcctgggcgg tgaggaggat gagctgcctg
     3781 ggctgggggg cgcccccttg cagggcctca ccctgcccaa caaggccacg cttggccact
     3841 tttatgagcg ctgcatccgg ggggccacgg ccgatgcagc ccgtacccgg gagttcctgg
     3901 aaggcttcgt ggatgacttg ctggaagccc tgaggagcct ctgcaaccgg gacaccgaca
     3961 tggaggtgga ggacttcatt ggcgtggaca gcatgtacga gaactggcag gtggacaggc
     4021 cactgctgtg ccaccttttc gtgcccttca caccccccga gccctaccgc ttccacccag
     4081 agctctggtg ctccggccgc tcagtgcccc tggatcgcca gggctacggc cagatcaagg
     4141 tggtccgcgc cgatggggac acattgagct gcatctgcgg caagaccaag ctcggggaag
     4201 acatgctgtg tctcctgcac ggcaggaaca gcatggcgcc tccctgcggc gacatggaga
     4261 acctgctgtg tgccacagat tccctgtacc tggacacgat gcaggtcatg aagtggttcc
     4321 agacggccct caccagagcc tggaagggca tcgcccacaa gtacgagttc gacctggcct
     4381 ttggccagct ggacagcccg gggtccctga agatcaagtt ccgttcaggg aagttcatgc
     4441 ccttcaacct gattcctgtg atccagtgtg atgactcgga cctgtacttt gtctcccacc
     4501 ttcccaggga gccctctgag ggcaccccag cctccagcac agactggctc ctgtcctttg
     4561 ctgtctatga gcgacacttc ctcaggacga cactaaaggc actgcccgag ggcgcctgcc
     4621 acctcagctg cctgcagata gcatccttcc tgctctccaa gcagagccgc ctgaccggtc
     4681 ccagcgggct cagcagctac cacctgaaga cggccctact gcacctccta ctcctccggc
     4741 aggccgccga ctggaaggcg gggcagctgg acgctcgtct gcacgagttg ctgtgcttcc
     4801 tggagaagag cttgctccag aagaagctcc accacttctt catcggcaac cgcaaggtgc
     4861 ctgaggccat gggactccct gaggccgtgc tcagggccga gcccctcaac ctcttccggc
     4921 ccttcgtcct gcagcgaagc ctttaccgta agacactgga ctccttctat gagatgctca
     4981 agaatgcccc agcgctcatt agcgagtatt ccctacatgt cccctcagac cagcctaccc
     5041 caaaaagcgg tgggccgggc gggggtggcg ggggaggtaa gcctatccct aaccctctcc
     5101 tcggtctcga ttctacgtga gtttaaactt cgaaacgcgt gaattcctcg agggcggccg
     5161 ctctagagtc gacgggccgc ggtaacaatt gttaactaac ttaagctagc aacggtttcc
     5221 ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     5281 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5341 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5401 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5461 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5521 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     5581 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5641 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     5701 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     5761 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgac cgagtacaag
     5821 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5881 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5941 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6001 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6061 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6121 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6181 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6241 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6301 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6361 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacccgg gcggggcgcg
     6421 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6481 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6541 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6601 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6661 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6721 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6781 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6841 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6901 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6961 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7021 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7081 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggtttttc
     7141 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7201 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7261 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7321 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7381 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7441 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7501 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7561 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7621 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7921 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     7981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8941 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9001 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9061 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10141 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10261 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10321 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
    10381 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10441 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10501 tagtagt
//
