LOCUS Exported 8857 bp ds-DNA circular SYN 16-MAR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 8857) AUTHORS Liza Brusman TITLE Direct Submission JOURNAL Exported Nov 12, 2018 from SnapGene Viewer 4.2.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8857 /organism="recombinant plasmid" /mol_type="other DNA" misc_structure 2028..2151 /label=cPPT /note="/vntifkey=88" promoter 2209..3380 /label=EF-1a /note="/vntifkey=29" primer_bind 3328..3348 /label=EF1a-F misc_feature 3391..3415 /label=C-term LIC misc_feature 3408..3425 /label=6X Gly CDS 3426..3467 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3497..4155 /label=IRES /note="/vntifkey=21" primer_bind complement(3676..3695) /label=IRES-R Addgene primer_bind complement(3748..3765) /label=IRES-R Jakki CDS 4156..4755 /codon_start=1 /label=Puro /note="/vntifkey=4" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4779..5370 /label=WPRE /note="/vntifkey=21" CDS complement(7495..8355) /codon_start=1 /label=AmpR /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgggccgggc gggggtggcg 3421 ggggaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtga gtttaaactt 3481 cgaaacgcgt gaattcctcg agggcggccg ctctagagtc gacgggccgc ggtaacaatt 3541 gttaactaac ttaagctagc aacggtttcc ctctagcggg atcaattccg cccccccccc 3601 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 3661 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 3721 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 3781 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 3841 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 3901 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 3961 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 4021 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 4081 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 4141 aaacacgata ataccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4201 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4261 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4321 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4381 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4441 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4501 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4561 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4621 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4681 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4741 aagcccggtg cctgacccgg gcggggcgcg tctggaacaa tcaacctctg gattacaaaa 4801 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 4861 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4921 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4981 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 5041 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5101 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 5161 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 5221 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5281 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5341 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 5401 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 5461 agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa 5521 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 5581 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 5641 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 5701 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 5761 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 5821 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 5881 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 5941 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 6001 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 6061 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 6121 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 6181 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 6241 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt 6301 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 6361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6541 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7141 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7261 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7321 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7381 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7441 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7501 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7561 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7621 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7681 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7741 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7801 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7861 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7921 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7981 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8041 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8101 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8161 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8221 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8281 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8341 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8401 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8461 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg 8521 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 8581 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 8641 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 8701 ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt 8761 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 8821 attgtatttg ttaaatatgt actacaaact tagtagt //