LOCUS       Exported                5220 bp ds-DNA     circular SYN 23-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jan 23, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2171..2190)
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink vntifkey=28 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2239..2379
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     promoter        2400..2871
                     /label=U6
                     /note="ApEinfo_fwdcolor=pink vntifkey=29 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2895..2981
                     /label=F+E Sp scaffold
                     /note="ApEinfo_fwdcolor=#0080c0 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     misc_feature    2895..2981
                     /label=misc_feature
                     /note="3'overhang=GGCC ugene_name=Fragment 3 
                     5'overhang=GTAC 3'type=sticky fragment_source=_0443 pBS H1 
                     GFP ugene_group=fragments ApEinfo_fwdcolor=pink 
                     3'strand=rev-compl 3'term=NotI 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink 5'term=KpnI 
                     5'strand=rev-compl 5'type=sticky"
     polyA_signal    complement(2982..2987)
                     /label=6xT
                     /note="ApEinfo_fwdcolor=pink vntifkey=25 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     3'UTR           3001..3016
                     /label=SV40 pA
                     /note="SV40 pA"
     enhancer        3023..3402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3403..3606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3651..3669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3700..3723
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3736..3999
                     /codon_start=1
                     /label=AcrIIA4
                     /translation="MNINDLIREIKNKDYTVKLSGTDSNSITQLIIRVNNDGNEYVISE
                     SENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELN"
     misc_feature    4038..4059
                     /label=mir122-5p
     misc_feature    4066..4087
                     /label=mir122-5p
     polyA_signal    4143..4367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(4391..4531)
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     primer_bind     complement(4574..4596)
                     /label=T7 Primer
                     /note="ApEinfo_fwdcolor=pink vntifkey=28 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtatgcatc
     2221 ctgcaggacg cgtcctaccc actccctcta tgcgcgctcg ctcactcact cggccctgga
     2281 gaccaaaggt ctccagactg ccggcctctg gccggcaggg ccgagtgagt gagcgagcgc
     2341 gcatagaggg agtgggtacc tccatcatct aggtttgcca gatctgatat cggcgcgccc
     2401 gagtccaaca cccgtgggaa tcccatgggc accatggccc ctcgctccaa aaatgctttc
     2461 gcgtcgcgca gacactgctc ggtagtttcg gggatcagcg tttgagtaag agcccgcgtc
     2521 tgaaccctcc gcgccgcccc ggccccagtg gaaagacgcg caggcaaaac gcaccacgtg
     2581 acggagcgtg accgcgcgcc gagcgcgcgc caaggtcggg caggaagagg gcctatttcc
     2641 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     2701 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     2761 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     2821 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgagtc
     2881 ttcgaggaag acaggtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     2941 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgcg gccgcggccg
     3001 ctctacaaat gtggtatcta gagacattga ttattgacta gttattaata gtaatcaatt
     3061 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     3301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     3361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     3421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     3481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     3541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     3601 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     3661 cactataggg agacccaagc tggctagtcc gccaccatgg actataagga cgatgacgat
     3721 aaagctggcg gcagcatgaa cattaacgac ctgattcggg aaataaagaa caaggactac
     3781 acggtcaagc tgtcaggcac cgactccaac tctataacac agctcattat aagggttaat
     3841 aatgatggca acgaatatgt catctctgaa tccgagaatg aatcaatcgt ggagaaattc
     3901 atcagtgcat ttaaaaacgg ctggaaccag gagtacgaag atgaggaaga gttctacaat
     3961 gatatgcaaa ctatcacact gaaatctgag ctgaactagc tcgagcggcc gccactgtgc
     4021 tggatatctg cagaattcaa acaccattgt cacactccat tatatcaaac accattgtca
     4081 cactccatta attagagacg agctcgtctc tagcttaagt ttaaaccgct gatcagcctc
     4141 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4201 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4261 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4321 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgggtc gactcgacga
     4381 tatcagatct aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4441 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
     4501 gtgagcgagc gagcgcgcag agagggagtg gccccccccc cccccccccc ctgcattcta
     4561 gagagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4741 cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4801 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4861 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4921 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4981 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     5041 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     5101 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     5161 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
//
