LOCUS       Exported                5237 bp ds-DNA     circular SYN 23-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jan 23, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2171..2190)
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink vntifkey=28 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2239..2379
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     promoter        2400..2871
                     /label=U6
                     /note="ApEinfo_fwdcolor=pink vntifkey=29 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2627..2871
                     /label=short U6 Variant FZ
     promoter        2627..2867
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2895..2981
                     /label=F+E Sp scaffold
                     /note="ApEinfo_fwdcolor=#0080c0 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     misc_feature    2895..2981
                     /label=misc_feature
                     /note="3'overhang=GGCC ugene_name=Fragment 3 
                     5'overhang=GTAC 3'type=sticky fragment_source=_0443 pBS H1 
                     GFP ugene_group=fragments ApEinfo_fwdcolor=pink 
                     3'strand=rev-compl 3'term=NotI 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink 5'term=KpnI 
                     5'strand=rev-compl 5'type=sticky"
     polyA_signal    complement(2982..2987)
                     /label=6xT
                     /note="ApEinfo_fwdcolor=pink vntifkey=25 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     enhancer        2996..3375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3376..3579
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3624..3642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3662..3667
     CDS             3668..4021
                     /codon_start=1
                     /label=AcrIIC3
                     /translation="MAFKRAIIFTSFNGFEKVSRTEKRRLAKIINARVSIIDEYLRAKD
                     TNASLDGQYRAFLFNDESPAMTEFLAKLKAFAESCTGISIDAWEIEESEYVRLPVERRD
                     FLAAANGKEIFKI"
     polyA_signal    4165..4389
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(4408..4548)
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     primer_bind     complement(4591..4613)
                     /label=T7 Primer
                     /note="ApEinfo_fwdcolor=pink vntifkey=28 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtatgcatc
     2221 ctgcaggacg cgtcctaccc actccctcta tgcgcgctcg ctcactcact cggccctgga
     2281 gaccaaaggt ctccagactg ccggcctctg gccggcaggg ccgagtgagt gagcgagcgc
     2341 gcatagaggg agtgggtacc tccatcatct aggtttgcca gatctgatat cggcgcgccc
     2401 gagtccaaca cccgtgggaa tcccatgggc accatggccc ctcgctccaa aaatgctttc
     2461 gcgtcgcgca gacactgctc ggtagtttcg gggatcagcg tttgagtaag agcccgcgtc
     2521 tgaaccctcc gcgccgcccc ggccccagtg gaaagacgcg caggcaaaac gcaccacgtg
     2581 acggagcgtg accgcgcgcc gagcgcgcgc caaggtcggg caggaagagg gcctatttcc
     2641 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     2701 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     2761 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     2821 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgagtc
     2881 ttcgaggaag acaggtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     2941 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgcg gccgcgacat
     3001 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3061 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3121 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3181 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3241 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3301 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3361 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3421 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     3481 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     3541 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     3601 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     3661 cgccaccatg gctttcaaga gagctattat atttacgtcc tttaatggtt ttgagaaggt
     3721 ctctcgaaca gagaagcgac gccttgccaa gataattaac gcgagggtct caattattga
     3781 tgagtatctc agagccaagg atacaaacgc gagcctggat ggccaatacc gagcctttct
     3841 gtttaacgac gaaagccccg cgatgacaga gttcctcgca aaactgaaag cattcgcgga
     3901 gagctgcacg ggaatatcaa tagatgcgtg ggagattgaa gagagcgagt atgttcgact
     3961 gcccgttgaa cgccgggatt tcctggccgc tgcgaatggc aaggaaatct tcaagatata
     4021 actcgagcgg ccgccactgt gctggatatc tgcagaattc aaacaccatt gtcacactcc
     4081 attatatcaa acaccattgt cacactccat taattagaga cgagctcgtc tctagcttaa
     4141 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4201 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4261 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4321 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4381 gctctatggg tcgacgatat cagatctagg aacccctagt gatggagttg gccactccct
     4441 ctctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
     4501 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc cccccccccc
     4561 cccccccctg cattctagag agctccaatt cgccctatag tgagtcgtat tacgcgcgct
     4621 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4681 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4741 gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt
     4801 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4861 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     4921 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4981 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     5041 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     5101 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5161 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     5221 gctacagggc gcgtcag
//
