LOCUS       Exported                3374 bp ds-DNA     circular SYN 14-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
COMMENT     RH170629/ovol
            
            #3 stock
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             683..1486
                     /codon_start=1
                     /label=mOvol1
                     /translation="MPRAFLVKKPCVSTCKRNWSELPDEERGEIYVPVSLGFCPPQPYR
                     EPEASVAEPPSCPLALDMSLRDSSYSVAPGPCVVAQLPSEDVSHLTDPQSRDQGFLRTK
                     MKVTLGDSPNGDLFTCHICQKSFTHQRMLNRHMKCHNDVKRHLCTYCGKGFNDTFDLKR
                     HVRTHTGVRPYKCSLCDKAFTQRCSLESHLKKIHGVQQKYAYKERRAKLYVCEECGCTS
                     ESQEGHVLHLKERHPDSPLLRKTSKKVAVALQNTVTSLLQGSPHL"
     protein_bind    complement(1499..1598)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1616..1634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1639..1655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1768..2577
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2724..3312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgcca ccatgcctcg cgcgttcctg gtgaagaagc catgcgtctc
      721 cacgtgcaag aggaactgga gcgagctccc ggacgaagag cgcggcgaga tctacgtgcc
      781 agtcagcctc ggcttctgcc caccacagcc ctaccgggaa ccagaggcat cggtggccga
      841 gcctccttcc tgccccctgg ctttggacat gagccttcga gactccagct atagcgtggc
      901 tccagggccc tgcgtggtgg cccagctacc ctctgaagat gtgagccatc tgacagatcc
      961 ccagagcaga gaccagggct ttctacgaac caagatgaag gtgaccttgg gggacagtcc
     1021 aaatggagac ctttttacct gccacatctg ccagaagtcc ttcacccacc agcgcatgct
     1081 gaatcgacac atgaagtgtc acaacgacgt caagaggcac ctctgcactt actgtgggaa
     1141 gggcttcaat gacacctttg acctcaagag acatgtccga acccacactg gtgtgcggcc
     1201 ctacaagtgc agtctgtgtg ataaggcctt cactcagcgc tgctctctgg agtcccacct
     1261 caagaagatc cacggagtgc agcagaagta tgcatacaag gagcgccggg ccaagctcta
     1321 cgtgtgtgag gagtgcggtt gcacatccga gagtcaagag ggtcatgtcc tgcacctcaa
     1381 ggaacgccac cccgacagcc cactgctgcg caagacctcc aagaaagtgg ctgtggctct
     1441 gcagaacact gtcacgtccc tgctgcaggg cagcccccat ctctgaagag gcgcgccgac
     1501 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1561 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1621 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1681 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct
     1741 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1801 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1861 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1921 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1981 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2041 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2101 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2161 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2221 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2281 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2341 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2401 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2461 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2521 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2581 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2641 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3361 tttgctcaca tgtt
//
