LOCUS       Exported                3469 bp ds-DNA     circular SYN 19-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2018 from SnapGene 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..7
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     15..31
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             27..350
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /note="color: #993366"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    44..100
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     complement(101..117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        591..695
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             696..1556
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  696 ..  764 / #ccffcc / signal sequence
                        2:  765 .. 1556 / #ccffcc
                     Cleavage site after base 764"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1727..2315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    2681..2689
                     /label=-10
                     /note="ApEinfo_fwdcolor=#c6c9d1
                     ApEinfo_revcolor=#c6c9d1"
                     /note="color: #c6c9d1"
     misc_feature    2700..2729
                     /label=Promoter score 8.4
                     /note="ApEinfo_fwdcolor=#75c6a9
                     ApEinfo_revcolor=#75c6a9"
                     /note="color: #75c6a9"
     misc_feature    2708..2732
                     /label=Repeat
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    2751..2775
                     /label=Repeat
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    2794..2818
                     /label=Repeat
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    2837..2842
                     /label=-35
                     /note="ApEinfo_fwdcolor=#f8d3a9
                     ApEinfo_revcolor=#f8d3a9"
                     /note="color: #f8d3a9"
     misc_feature    2854..2862
                     /label=-10, score 75
                     /note="ApEinfo_fwdcolor=#f58a5e
                     ApEinfo_revcolor=#f58a5e"
                     /note="color: #f58a5e"
     misc_feature    complement(2855..2863)
                     /label=-10 Score 69
                     /note="ApEinfo_fwdcolor=#75c6a9
                     ApEinfo_revcolor=#75c6a9"
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2881..2886)
                     /label=-35
                     /note="ApEinfo_fwdcolor=#c6c9d1
                     ApEinfo_revcolor=#c6c9d1"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2999..3023
                     /label=Repeat
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    3042..3066
                     /label=Repeat
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
ORIGIN
        1 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
       61 ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac
      121 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      181 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      241 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      301 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgctac
     2521 gtgccgatca acgtatcatt ttcgccagat atcgacgaag agccttcatc acagaaagct
     2581 tgaggaagag cgtctccgga gtccggagac gctgcaatga tggtcatata cgataaatta
     2641 gggacgcaga agaccgatcc ttcccggatc aacaatggac gtctagaaag caggtcttct
     2701 ttactttcct tataaaccct tccaataatg gggagaccac gacaggtctc ccttataaac
     2761 ccttccaata atgggccaga gtttggtatt gatccttata aacccttcca ataatgggag
     2821 actagcaaat atctatttga aagtcaatta taaaagtata ataaatttgt ttaatatgtg
     2881 attaaacttg atagaaaata tattaaaaag ttaaataaat ggtgaggggc taaatacaat
     2941 aatatgactg tatttagccc ctaatttatt tagtaactgt agggattatg agttttagcc
     3001 ttataaaccc ttccaataat gggccagagt ttggtattga tccttataaa cccttccaat
     3061 aatgggccgg cacttctatc tggaacctta taaacccttc caataatgga gaatagcaaa
     3121 tatcatattg attgtcaata aaaaattaga aagtttgatg ctaaattgaa gaataaatta
     3181 attggaaaaa tggattaaaa cactacaaag catgaagttg aggattcggc tattttcagc
     3241 cgagagcgta gaggtaaatt ctatcgctga gacgattgac aataagagtg tagtttaagt
     3301 ttctctctaa agggaaaagt gacaattctt cggctgcaag agacaaaaag gattgtcctc
     3361 attgatttac tttaggtaaa attaccagat tagggttctg ttggactctt ctaactttcg
     3421 gagaaaaaac tagagtcaca ctggctcacc ttcgggtggg cctttctgc
//
