LOCUS       Exported               10530 bp ds-DNA     circular SYN 21-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1(-)-HF2-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10530
                     /organism="synthetic DNA construct"
     promoter        232..819
                     /label=CMV promoter
     promoter        863..879
                     /label=T7 promoter
     misc_feature    927..6059
                     /label=HF2-BE3
     polyA_site      6130..6354
                     /label=BGH pA
     rep_origin      6400..6828
                     /direction=RIGHT
                     /label=f1 origin
     promoter        6833..7202
                     /label=SV40 early promoter
     CDS             7238..8032
                     /codon_start=1
                     /label=Neo(R)
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8206..8336
                     /label=SV40 pA
     rep_origin      complement(8719..9389)
                     /direction=LEFT
                     /label=pUC origin
     CDS             complement(9534..10394)
                     /codon_start=1
                     /label=Amp(R)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10395..10493)
                     /label=bla promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga gccaccatga gctcagagac tggcccagtg gctgtggacc
      961 ccacattgag acggcggatc gagccccatg agtttgaggt attcttcgat ccgagagagc
     1021 tccgcaagga gacctgcctg ctttacgaaa ttaattgggg gggccggcac tccatttggc
     1081 gacatacatc acagaacact aacaagcacg tcgaagtcaa cttcatcgag aagttcacga
     1141 cagaaagata tttctgtccg aacacaaggt gcagcattac ctggtttctc agctggagcc
     1201 catgcggcga atgtagtagg gccatcactg aattcctgtc aaggtatccc cacgtcactc
     1261 tgtttattta catcgcaagg ctgtaccacc acgctgaccc ccgcaatcga caaggcctgc
     1321 gggatttgat ctcttcaggt gtgactatcc aaattatgac tgagcaggag tcaggatact
     1381 gctggagaaa ctttgtgaat tatagcccga gtaatgaagc ccactggcct aggtatcccc
     1441 atctgtgggt acgactgtac gttcttgaac tgtactgcat catactgggc ctgcctcctt
     1501 gtctcaacat tctgagaagg aagcagccac agctgacatt ctttaccatc gctcttcagt
     1561 cttgtcatta ccagcgactg cccccacaca ttctctgggc caccgggttg aaaagcggca
     1621 gcgagactcc cgggacctca gagtccgcca cacccgaaag tgataaaaag tattctattg
     1681 gtttagccat cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac
     1741 cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta
     1801 tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg
     1861 ctcggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca
     1921 atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg
     1981 aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat
     2041 atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata
     2101 aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact
     2161 ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt
     2221 tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg
     2281 cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac
     2341 aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc
     2401 tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta
     2461 aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg
     2521 acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag
     2581 ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac
     2641 atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata
     2701 aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga
     2761 gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag
     2821 agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg
     2881 gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg
     2941 atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca
     3001 taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa
     3061 agtccgaaga aacgattact ccctggaatt ttgaggaagt tgtcgataaa ggtgcgtcag
     3121 ctcaatcgtt catcgagagg atgaccgcct ttgacaagaa tttaccgaac gaaaaagtat
     3181 tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta
     3241 agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa
     3301 tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact
     3361 actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta
     3421 atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg
     3481 ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag
     3541 atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta
     3601 tgaaacagtt aaagaggcgt cgctatacgg gctggggagc cttgtcgcgg aaacttatca
     3661 acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct
     3721 tcgccaatag gaactttatg gccctgatcc atgatgactc tttaaccttc aaagaggata
     3781 tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg
     3841 ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag
     3901 ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc
     3961 aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta
     4021 ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga
     4081 acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac
     4141 tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa tcctttttga
     4201 aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg
     4261 acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa
     4321 atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct
     4381 tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgcgccatca
     4441 caaagcatgt tgcccagata ctagattccc gaatgaatac gaaatacgac gagaacgata
     4501 agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa
     4561 aggattttca attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt
     4621 atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt
     4681 ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg
     4741 agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga
     4801 cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg
     4861 agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt
     4921 ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg
     4981 aatcgattct tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc
     5041 cgaaaaagta cggtggcttc gagagcccta cagttgccta ttctgtccta gtagtggcaa
     5101 aagttgagaa gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga
     5161 ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca
     5221 aggaagtaaa aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa
     5281 atggccgaaa acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac
     5341 taccgtctaa atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt
     5401 cacctgaaga taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg
     5461 aaatcataga gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg
     5521 acaaagtatt aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa
     5581 atattatcca tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg
     5641 acacaacgat agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga
     5701 ttcaccaatc catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg
     5761 actctggtgg ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg
     5821 ttatccagga atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc
     5881 cggaaagcga tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc
     5941 ttctgactag cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg
     6001 gtgagaacaa gattaagatg ctctctggtg gttctcccaa gaagaagagg aaagtctaat
     6061 tccaccacac tggactagtg gatccgagct cggtaccaag cttaagttta aaccgctgat
     6121 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6181 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6241 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6301 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6361 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6421 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6481 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6541 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6601 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6661 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6721 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6781 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     6841 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6901 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6961 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7021 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7081 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7141 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7201 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7261 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7321 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7381 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7441 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7501 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7561 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7621 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7681 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7741 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7801 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7861 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7921 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7981 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8041 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8101 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8161 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8221 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8281 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8341 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9301 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10141 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10501 acatttcccc gaaaagtgcc acctgacgtc
//
