LOCUS       Exported               10570 bp ds-DNA     circular SYN 21-NOV-2018
DEFINITION  Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    pET42b-ABE7.10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Rees HA, Komor AC, Yeh WH, Caetano-Lopes J, Warman M, Edge ASB, Liu 
            DR
  TITLE     Improving the DNA specificity and applicability of base editing 
            through protein engineering and protein delivery.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    39..1226
                     /label=Gene X
     misc_feature    39..536
                     /label=ecTadA-WT
     misc_feature    537..560
                     /label=SGGSSGGS
     misc_feature    561..608
                     /label=XTEN
     misc_feature    609..632
                     /label=SGGSSGGS
     misc_feature    633..1130
                     /label=ecTadA*
     misc_feature    696..698
                     /label=L23R
     misc_feature    735..737
                     /label=L36
     variation       771..773
                     /label=A48
     misc_feature    780..782
                     /label=L51
     misc_feature    879..881
                     /label=F84
     misc_feature    945..947
                     /label=V106
     misc_feature    951..953
                     /label=N108
     misc_feature    996..998
                     /label=Y123
     misc_feature    1053..1055
                     /label=N142A
     misc_feature    1065..1067
                     /label=C146
     misc_feature    1068..1070
                     /label=Y147
     misc_feature    1083..1085
                     /label=R152P
     misc_feature    1092..1094
                     /label=V155
     misc_feature    1095..1097
                     /label=F156
     misc_feature    1098..1100
                     /label=N157
     misc_feature    1131..1154
                     /label=SGGSSGGS
     misc_feature    1155..1202
                     /label=XTEN
     misc_feature    1203..1226
                     /label=SGGSSGGS
     CDS             1227..5327
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5328..5339
                     /label=SGGS
     CDS             5340..5360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     5426..5444
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5440..5487
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5524..5979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5611..5630
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5821..5842
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6071..6886)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6794..6813
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6878..6897
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7008..7596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7497..7516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7750..7767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7782..7921
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7847..7866
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8007..8029
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8023..8214)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     YELYRSCLARFGDDGENL"
     primer_bind     8682..8731
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(9023..10105)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNHASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQLLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESWQ"
     primer_bind     10067..10086
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10106..10183)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10389..10408
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     10492..10511
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10492..10510
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10511..10535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             join(10550..10570,1..2)
                     /label=RBS_1
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatataccat gggcagcagt catcatcatc accatcactc ggaggttgaa ttctcccacg
       61 agtattggat gcggcacgct cttacgttag caaaacgcgc gtgggacgag cgtgaagtac
      121 cggtaggcgc cgtgttagtg cataataacc gggtcattgg tgaaggatgg aatcggccga
      181 tcgggagaca cgatccgaca gcacatgctg agattatggc tttacggcaa ggaggactgg
      241 ttatgcagaa ctaccggttg attgatgcta cactgtacgt aaccttagaa ccatgtgtga
      301 tgtgtgctgg agccatgata cattcccgca tcggaagagt ggtttttggg gctcgtgatg
      361 caaaaactgg cgccgccgga agtcttatgg acgtgttaca tcatccaggc atgaatcatc
      421 gggtcgagat tacagagggc attttggcag atgaatgtgc tgcattgctt agtgatttct
      481 tccgcatgcg gagacaggaa atcaaagccc aaaaaaaagc tcaaagtagt actgatagtg
      541 gtggatccag tggaggctcg tcaggctctg aaacgcctgg cacatcagaa tcggcaacgc
      601 cagagtcgtc aggaggttcc tcaggtggat cttcggaggt cgagttttca catgagtatt
      661 ggatgcgtca tgccttgacg ttggcgaaac gggcgcgcga tgagcgtgag gtgcccgtgg
      721 gagcggtgtt ggtactgaat aaccgggtta taggggaagg atggaaccgg gctattgggt
      781 tacacgaccc aacggcgcac gccgagataa tggcactgcg ccaagggggc ttagttatgc
      841 agaattatcg ccttatcgat gctacactgt atgtaacctt tgaaccctgc gtaatgtgtg
      901 cgggggctat gatccactcg agaatagggc gcgtggtatt cggcgtacgc aacgctaaaa
      961 ccggggctgc gggctcgttg atggatgttc tgcactaccc cggaatgaat cacagagtag
     1021 agatcacgga gggaattttg gccgacgaat gtgcagcttt actgtgctac ttttttcgga
     1081 tgccgcggca agtcttcaac gcacagaaga aggctcaatc ttccactgac tcaggtggct
     1141 cgagtggtgg gagtagcgga tctgagacgc caggcacatc agagagtgca acccccgagt
     1201 catcgggtgg gagttccggc ggatctgata agaaatactc aataggctta gctatcggca
     1261 caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca
     1321 aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat
     1381 ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata
     1441 cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag
     1501 tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc
     1561 atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc
     1621 caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct
     1681 taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag
     1741 atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca
     1801 atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt
     1861 ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga
     1921 agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta
     1981 aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg
     2041 atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag
     2101 ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa
     2161 ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga
     2221 ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg
     2281 atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt
     2341 ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac
     2401 taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc
     2461 aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt
     2521 taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg
     2581 gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa
     2641 ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg
     2701 aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt
     2761 tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag
     2821 gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct
     2881 tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag
     2941 aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta
     3001 cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg
     3061 aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg
     3121 aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac
     3181 gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata
     3241 agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt
     3301 ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag
     3361 tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta
     3421 ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc
     3481 ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg
     3541 gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa
     3601 gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc
     3661 tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt
     3721 taagtgatta tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag
     3781 acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg
     3841 aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca
     3901 ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata
     3961 aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac
     4021 aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg
     4081 ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct
     4141 ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg
     4201 ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt
     4261 ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa
     4321 ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg
     4381 caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg
     4441 tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca
     4501 atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa
     4561 aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg
     4621 gttttgatag tccaacggta gcttattcag tcctagttgt tgctaaggtg gaaaaaggga
     4681 aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt
     4741 cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag
     4801 acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga
     4861 tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg
     4921 tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg
     4981 aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa
     5041 tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg
     5101 catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat
     5161 ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc
     5221 gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca
     5281 ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactct ggcgggtctc
     5341 ccaagaagaa gaggaaagtc taataattga ttaataccta ggctgctaaa caaagcccga
     5401 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     5461 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     5521 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5581 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5641 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5701 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5761 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5821 gtggactctt gttccaaact ggaacaacac tcaaccatat ctcggtctat tcttttgatt
     5881 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5941 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6001 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6061 gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     6121 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     6181 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     6241 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     6301 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     6361 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     6421 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     6481 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     6541 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     6601 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     6661 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     6721 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     6781 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     6841 ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     6901 tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt
     6961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7021 tttctgcgcg taatctgctg attgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7141 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7201 gcaccgccta catacctcgc tctgctaatc atgttaccag tggctgctgc cagtggcgat
     7261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7321 ggctgaacgg ggggttcgtg cacacagcca agcttggagc gaacgaccta caccgaactg
     7381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     7801 cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg
     7861 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     7921 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7981 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8041 atcaccgaaa cgcgcgaggc agctgcggta aagctcataa gcgtggtcgt gaagcgattc
     8101 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     8161 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     8221 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     8281 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     8341 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     8401 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     8461 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     8521 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     8581 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     8641 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat
     8701 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     8761 ggagtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     8821 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
     8881 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
     8941 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
     9001 acttacatta attgcgttgc gctcactgcc agctttccag tcgggaaacc tgtcgtgcca
     9061 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     9121 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     9181 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     9241 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     9301 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     9361 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     9421 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     9481 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     9541 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     9601 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     9661 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     9721 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     9781 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     9841 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     9901 aggtggcaac gccaatcagc aacaactgtt tgcccgccag ttgttgtgcc acgcggttgg
     9961 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
    10021 ggctggcatg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
    10081 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
    10141 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
    10201 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
    10261 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
    10321 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc caaagtggcg agcccgatct
    10381 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
    10441 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
    10501 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
    10561 ttaagaagga
//
