LOCUS       pCRISP_IS.xdna    9524 bp      DNA     circular   11/22/2018
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic circular DNA - [LOCUS]: Exported - [
            ACCESSION]: . - [GENE]: 2225..6331
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     rep_origin      620..1540
                     /label=p15A ori_rep_origin
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="p15 ori" - 620..1540 \ 
     gene            2225..6331
                     /label=Cas9
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="ORF2" - 2225..6331 \ 
     misc_feature    6484..6519
                     /label=CRISPR repeat
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="CRISPR repeat" - 6484..6519 \ 
     misc_feature    complement(6719..6724)
                     /label=BsaI target site
                     /SerialCloner_Color=&h8080C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="BsaI" - complement(6521..6526) \ 
     misc_feature    6550..6585
                     /label=CRISPR repeat
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="CRISPR repeat" - 6550..6585 \ 
     gene            7022..8050
                     /label=tet_gene
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="tet" - 6824..7852 \ 
     gene            complement(9084..219)
                     /label=CAT_gene
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="CAT" - complement(8886..219) \ 
     misc_feature    6616..6651
                     /label=CRISPR repeat
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6682..6717
                     /label=CRISPR repeat
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6520..6549
                     /label=IS1 silencer
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6586..6615
                     /label=IS5 silencer
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6652..6681
                     /label=IS3 silencer
                     /SerialCloner_Color=&h8080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     1645..1664
                     /label=CRISPathFW
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(8149..8169)
                     /label=CRISPathREV
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(6738..6757)
                     /label=CRISPcheckREV
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     6316..6335
                     /label=CRSIPcheckFW
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca 
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc 
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct 
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg 
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg 
     1681 attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag 
     1741 aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag 
     1801 cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc 
     1861 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg 
     1921 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa 
     1981 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact 
     2041 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga 
     2101 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa 
     2161 ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc 
     2221 aaaaatggat aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc 
     2281 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga 
     2341 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc 
     2401 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat 
     2461 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca 
     2521 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt 
     2581 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg 
     2641 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc 
     2701 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag 
     2761 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa 
     2821 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc 
     2881 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg 
     2941 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc 
     3001 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt 
     3061 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc 
     3121 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc 
     3181 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt 
     3241 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata 
     3301 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat 
     3361 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct 
     3421 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct 
     3481 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa 
     3541 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa 
     3601 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga 
     3661 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga 
     3721 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac 
     3781 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt 
     3841 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt 
     3901 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga 
     3961 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa 
     4021 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat 
     4081 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata 
     4141 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg 
     4201 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat 
     4261 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga 
     4321 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag 
     4381 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca 
     4441 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat 
     4501 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga 
     4561 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca 
     4621 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg 
     4681 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga 
     4741 tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg 
     4801 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat 
     4861 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa 
     4921 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg 
     4981 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat 
     5041 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa 
     5101 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa 
     5161 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa 
     5221 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg 
     5281 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta 
     5341 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa 
     5401 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga 
     5461 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga 
     5521 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct 
     5581 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt 
     5641 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc 
     5701 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat 
     5761 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc 
     5821 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga 
     5881 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc 
     5941 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt 
     6001 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg 
     6061 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga 
     6121 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg 
     6181 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac 
     6241 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg 
     6301 cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta 
     6361 ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga 
     6421 ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac 
     6481 gaggttttag agctatgctg ttttgaatgg tcccaaaacA TGCTGCCAAC TTACTGATTT 
     6541 AGTGTATGAg ttttagagct atgctgtttt gaatggtccc aaaacGGCTC CAGATGACAA 
     6601 ACATGATCTC ATATCgtttt agagctatgc tgttttgaat ggtcccaaaa cACGCGGCTA 
     6661 AGTGAGTAAA CTCTCAGTCA Ggttttagag ctatgctgtt ttgaatggtc ccaAAACtga 
     6721 gacctgtctc ggaagctcat aggactcgtt ttagagctat gctgttttga atggtcccaa 
     6781 aacttcagca cactgagact tgttgagttc catgttttag agctatgctg ttttgaatgg 
     6841 actccattca acattgccga tgataacttg agaaagaggg ttaataccag cagtcggata 
     6901 ccttcctatt ctttctgtta aagcgttttc atgttataat aggcaaaaga agagtagtgt 
     6961 gatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt 
     7021 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc 
     7081 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc 
     7141 cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc 
     7201 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg 
     7261 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt 
     7321 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct 
     7381 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt 
     7441 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 
     7501 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 
     7561 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 
     7621 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 
     7681 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 
     7741 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 
     7801 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 
     7861 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat 
     7921 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 
     7981 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 
     8041 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga 
     8101 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 
     8161 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg 
     8221 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc 
     8281 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc 
     8341 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt 
     8401 ctggaaacgc ggaagtcccc tacgtgctgc tgaagttgcc cgcaacagag agtggaacca 
     8461 accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct catttcttat 
     8521 tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc gcggggcatg 
     8581 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 
     8641 gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg 
     8701 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 
     8761 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa 
     8821 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat 
     8881 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga 
     8941 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga 
     9001 atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg 
     9061 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt 
     9121 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc 
     9181 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg 
     9241 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga 
     9301 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca 
     9361 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat 
     9421 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga 
     9481 acactatccc atatcaccag ctcaccgtct ttcattgcca tacg
//